Hydrophobic pore space constituted in macroporous ZIF-8 for lipase immobilization greatly improving lipase catalytic performance in biodiesel preparation

Hu, Yingli; Dai, Lingmei; Liu, Dehua

doi:10.1186/s13068-020-01724-w

Cited by 47 publications

(35 citation statements)

References 31 publications

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“…With ZIF-8 however, the small micropores prevented the ANL molecules from entering the pores, which results in only surface adsorption of ANL@ZIF-8. The enzyme activity and activity recovery of ANL@M-ZIF-8 were 7-and 10-fold higher than those found in our previous work 18 with the same enzyme, ANL, surface immobilized on UiO-66. The enzymatic activity of ANL@M-ZIF-8 was also much higher than that of the corresponding free lipase, which is about 5.45 U/mg.…”

Section: Resultscontrasting

confidence: 67%

Lipase Immobilization on Macroporous ZIF-8 for Enhanced Enzymatic Biodiesel Production

Zhou

Dai

et al. 2021

ACS Omega

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Immobilization of enzyme on metal−organic frameworks (MOFs) has drawn increasing interest owing to their many well-recognized characteristics. However, the pore sizes of MOFs (mostly micropores and mesopores) limit their application for enzyme immobilization to a great extent owing to the large size of enzyme molecules. Synthesis of MOFs with macropores would therefore solve this problem, typically encountered with conventional MOFs. In this work, macroporous zeolitic imidazolate frameworks (ZIF-8), referred to as M-ZIF-8, were synthesized and used for immobilization of Aspergillus niger lipase (ANL). Immobilization efficiency using M-ZIF-8 and enzymatic catalytic performance for biodiesel preparation were investigated. The immobilized ANL on M-ZIF-8 (ANL@M-ZIF-8) showed higher enzymatic activity (6.5-fold), activity recovery (3.8-fold), thermal stability (1.4-and 3.4-fold at 80 and 100 °C, respectively), reusability (after five cycles, 68% of initial activity was maintained), and porosity than ANL on conventional ZIF-8 (ANL/ZIF-8). In addition, by using ANL@M-ZIF-8 for catalyzing a biodiesel production reaction, a higher fatty acid methyl ester yield was achieved.

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Section: Resultscontrasting

confidence: 67%

Lipase Immobilization on Macroporous ZIF-8 for Enhanced Enzymatic Biodiesel Production

Zhou

Dai

et al. 2021

ACS Omega

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“…In another study, macroporous ZIF-8 was modified with PDMS in order to builda hydrophobic pore space for lipase from Aspergillus niger immobilization. Immobilized lipase showed improved stability and was found as an effective biocatalyst in the transesterification process in biodiesel production [59].…”

Section: Immobilized Lipase Characterizationmentioning

confidence: 99%

Pristine and Poly(Dimethylsiloxane) Modified Multi-Walled Carbon Nanotubes as Supports for Lipase Immobilization

et al. 2021

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The presented study deals with the fabrication of highly stable and active nanobiocatalysts based on Candida antarctica lipase B (CALB) immobilization onto pristine and poly(dimethylsiloxane) modified MWCNTs. The MWCNTs/PDMS nanocomposites, containing 40 wt. % of the polymer with two molecular weights, were successfully synthesized via adsorption modification. The effect of PDMS chains length on the textural/structural properties of produced materials was studied by means of the nitrogen adsorption–desorption technique, Raman spectroscopy, and attenuated total reflectance Fourier transform infrared spectroscopy. P-MWCNTs and MWCNTs/PDMS nanocomposites were tested as supports for lipase immobilization. Successful deposition of the enzyme onto the surface of P-MWCNTs and MWCNTs/PDMS nanocomposite materials was confirmed mainly using ATR-FTIR spectroscopy. The immobilization efficiency, stability, and catalytic activity of the immobilized enzyme were studied,and the reusability of the produced biocatalytic systems was examined. The presented results demonstrate that the produced novel biocatalysts might be considered as promising materials for biocatalytic applications.

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“…In recent years, porous materials have been developed and applied to integrate enzymes based on their excellent features of high surface area and porosity which facilitate the immobilization process and preservation of biomolecules [ 8 ]. Metal–Organic Frameworks (MOFs) with high surface area, pore channels, and chemical functionality [ 9 – 11 ] has been reported to entrap lipase for biodiesel [ 12 – 14 ]. However, MOFs is chemically unstable and toxic due to the metal ions, since some are water-sensitive or easily digested [ 15 – 17 ].…”

Section: Introductionmentioning

confidence: 99%

Magnetic COFs as satisfactory support for lipase immobilization and recovery to effectively achieve the production of biodiesel by maintenance of enzyme activity

Zhou

Cai

Xing

et al. 2021

Biotechnol Biofuels

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Background Production of biodiesel from renewable sources such as inedible vegetable oils by enzymatic catalysis has been a hotspot but remains a challenge on the efficient use of an enzyme. COFs (Covalent Organic Frameworks) with large surface area and porosity can be applied as ideal support to avoid aggregation of lipase and methanol. However, the naturally low density limits its application. In this work, we reported a facile synthesis of core–shell magnetic COF composite (Fe3O4@COF-OMe) to immobilize RML (Rhizomucor miehei lipase), to achieve its utilization in biodiesel production. Result This strategy gives extrinsic magnetic property, and the magnetic COFs is much heavier and could disperse in water medium well, facilitating the attachment with the enzyme. The resultant biocomposite exhibited an excellent capacity of RML due to its high surface area and fast response to the external magnetic field, as well as good chemical stability. The core–shell magnetic COF-OMe structure not only achieved highly efficient immobilization and recovery processes but also maintained the activity of lipase to a great extent. RML@Fe3O4@COF-OMe performed well in practical applications, while free lipase did not. The biocomposite successfully achieved the production of biodiesel from Jatropha curcas Oil with a yield of about 70% in the optimized conditions. Conclusion Magnetic COFs (Fe3O4@COF-OMe) for RML immobilization greatly improved catalytic performance in template reaction and biodiesel preparation. The magneticity makes it easily recovered and separated from the system. This first successful attempt of COFs-based immobilized enzyme broadened the prospect of biodiesel production by COFs with some inspiration.

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Hydrophobic pore space constituted in macroporous ZIF-8 for lipase immobilization greatly improving lipase catalytic performance in biodiesel preparation

Cited by 47 publications

References 31 publications

Lipase Immobilization on Macroporous ZIF-8 for Enhanced Enzymatic Biodiesel Production

Lipase Immobilization on Macroporous ZIF-8 for Enhanced Enzymatic Biodiesel Production

Pristine and Poly(Dimethylsiloxane) Modified Multi-Walled Carbon Nanotubes as Supports for Lipase Immobilization

Magnetic COFs as satisfactory support for lipase immobilization and recovery to effectively achieve the production of biodiesel by maintenance of enzyme activity

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