Hydrophobicity-driven self-assembly of protein and silver nanoparticles for protein detection using surface-enhanced Raman scattering

Kahraman, Mehmet; Balz, Ben Niklas; Wachsmann‐Hogiu, Sebastian

doi:10.1039/c3an00025g

Cited by 26 publications

(23 citation statements)

References 61 publications

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“…98 Kahraman et al also observed a LOD of 0.5 μg/mL for BSA, Hb, thrombin, avidin, cytochrome c, and lysozyme by using hydrophobicity-driven self-assembly. 99 For quantification, a reference peak was chosen for each protein: lysozyme (760 cm −1 ), cytochrome c (1005 and 1262 cm −1 ), and avidin (945 cm −1 ). 99 …”

Section: Amino Acids Peptides and Proteinsmentioning

confidence: 99%

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Bioanalytical applications of surface-enhanced Raman spectroscopy: de novo molecular identification

Nguyen

Peters

Schultz

2017

Reviews in Analytical Chemistry

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Surface enhanced Raman scattering (SERS) has become a powerful technique for trace analysis of biomolecules. The use of SERS-tags has evolved into clinical diagnostics, the enhancement of the intrinsic signal of biomolecules on SERS active materials shows tremendous promise for the analysis of biomolecules and potential biomedical assays. The detection of the de novo signal from a wide range of biomolecules has been reported to date. In this review, we examine different classes of biomolecules for the signals observed and experimental details that enable their detection. In particular, we survey nucleic acids, amino acids, peptides, proteins, metabolites, and pathogens. The signals observed show that the interaction of the biomolecule with the enhancing nanostructure has a significant influence on the observed spectrum. Additional experiments demonstrate that internal standards can correct for intensity fluctuations and provide quantitative analysis. Experimental methods that control the interaction at the surface are providing for reproducible SERS signals. Results suggest that combining advances in methodology with the development of libraries for SERS spectra may enable the characterization of biomolecules complementary to other existing methods.

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Section: Amino Acids Peptides and Proteinsmentioning

confidence: 99%

“…99 For quantification, a reference peak was chosen for each protein: lysozyme (760 cm −1 ), cytochrome c (1005 and 1262 cm −1 ), and avidin (945 cm −1 ). 99 …”

Section: Amino Acids Peptides and Proteinsmentioning

confidence: 99%

Bioanalytical applications of surface-enhanced Raman spectroscopy: de novo molecular identification

Nguyen

Peters

Schultz

2017

Reviews in Analytical Chemistry

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“…A novel method based on the aggregation of suspended droplet of mixture containing AgNPs and proteins from a hydrophobic surface was reported for the label-free detection of proteins with a LOD down to 0.05 μg/ml for the model proteins [216]. A simple sample preparation method for sensitive (LOD 0.5 μg/ml) and reproducible label-free detection of proteins based on the self-assembly of AgNPs and proteins on hydrophobic surfaces was also demonstrated [217].…”

Section: Sers Of Proteinsmentioning

confidence: 99%

Fundamentals and applications of SERS-based bioanalytical sensing

et al. 2017

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Abstract:Plasmonics is an emerging field that examines the interaction between light and metallic nanostructures at the metal-dielectric interface. Surface-enhanced Raman scattering (SERS) is a powerful analytical technique that uses plasmonics to obtain detailed chemical information of molecules or molecular assemblies adsorbed or attached to nanostructured metallic surfaces. For bioanalytical applications, these surfaces are engineered to optimize for high enhancement factors and molecular specificity. In this review we focus on the fabrication of SERS substrates and their use for bioanalytical applications. We review the fundamental mechanisms of SERS and parameters governing SERS enhancement. We also discuss developments in the field of novel SERS substrates. This includes the use of different materials, sizes, shapes, and architectures to achieve high sensitivity and specificity as well as tunability or flexibility. Different fundamental approaches are discussed, such as label-free and functional assays. In addition, we highlight recent relevant advances for bioanalytical SERS applied to small molecules, proteins, DNA, and biologically relevant nanoparticles. Subsequently, we discuss the importance of data analysis and signal detection schemes to achieve smaller instruments with low cost for SERS-based point-of-care technology developments. Finally, we review the main advantages and challenges of SERS-based biosensing and provide a brief outlook.

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“…The interaction of LSPR modes with target biomolecules results in quantitative changes of optical signals and thus the metal nanoparticles are also called plasmonic nanoparticles, which offer the colorimetric and label‐free analysis of biomolecules. Meanwhile, the plasmonic nanoparticles are also utilized to significantly improve the fluorescence and Raman spectroscopies . Through means of staining, the nanoparticles can interact with analytes in a controllable and facile way, based on which various sensitive and flexible analytical methods have been developed.…”

Section: Introductionmentioning

confidence: 99%

Photonic Crystal Hydrogel Enhanced Plasmonic Staining for Multiplexed Protein Analysis

Zhao

Huang

et al. 2015

Small

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Plasmonic nanoparticles are commonly used as optical transducers in sensing applications. The optical signals resulting from the interaction of analytes and plamsonic nanoparticles are influenced by surrounding physical structures where the nanoparticles are located. This paper proposes inverse opal photonic crystal hydrogel as 3D structure to improve Raman signals from plasmonic staining. By hybridization of the plasmonic nanoparticles and photonic crystal, surface-enhanced Raman spectroscopy (SERS) analysis of multiplexed protein is realized. It benefits the Raman analysis by providing high-density "hot spots" in 3D and extra enhancement of local electromagnetic field at the band edge of PhC with periodic refractive index distribution. The strong interaction of light and the hybrid 3D nanostructure offers new insights into plasmonic nanoparticle applications and biosensor design.

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Hydrophobicity-driven self-assembly of protein and silver nanoparticles for protein detection using surface-enhanced Raman scattering

Cited by 26 publications

References 61 publications

Bioanalytical applications of surface-enhanced Raman spectroscopy: de novo molecular identification

Bioanalytical applications of surface-enhanced Raman spectroscopy: de novo molecular identification

Fundamentals and applications of SERS-based bioanalytical sensing

Photonic Crystal Hydrogel Enhanced Plasmonic Staining for Multiplexed Protein Analysis

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