Hydroquinone derivatives attenuate biofilm formation and virulence factor production in Vibrio spp

Sathiyamoorthi, Ezhaveni; Faleye, Olajide Sunday; Lee, Jin-Hyung; Lee, Jintae

doi:10.1016/j.ijfoodmicro.2022.109954

Cited by 9 publications

(13 citation statements)

References 83 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, documented inhibitory effects on the growth of pathogenic bacteria such as Pseudomona aeruginosa , Klebsiella pneumoniae , and Escherichia coli have been reported [ 69 , 70 , 71 ]. Additionally, studies on other bacteria within the genus Aliivibrio [ 72 , 73 ] observed antimicrobial activity of HQ derivatives. Interestingly, these derivatives appear to downregulate genes of Aliivibrio spp.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Hydroquinone Ecotoxicity: Unveiling Risks in Soil and River Ecosystems with Insights into Microbial Resilience

Valenzuela,

Ballestero,

Gan

et al. 2024

Toxics

View full text Add to dashboard Cite

Despite widespread industrial use, the environmental safety of hydroquinone (HQ), a benzene compound from plants used in processes like cosmetics, remains uncertain. This study evaluated the ecotoxicological impact of HQ on soil and river environments, utilizing non-target indicator organisms from diverse trophic levels: Daphnia magna, Aliivibrio fischeri, Allium cepa, and Eisenia fetida. For a more environmentally realistic assessment, microbial communities from a river and untreated soil underwent 16S rRNA gene sequencing, with growth and changes in community-level physiological profiling assessed using Biolog EcoPlate™ assays. The water indicator D. magna exhibited the highest sensitivity to HQ (EC50 = 0.142 µg/mL), followed by A. fischeri (EC50 = 1.446 µg/mL), and A. cepa (LC50 = 7.631 µg/mL), while E. fetida showed the highest resistance (EC50 = 234 mg/Kg). Remarkably, microbial communities mitigated HQ impact in both aquatic and terrestrial environments. River microorganisms displayed minimal inhibition, except for a significant reduction in polymer metabolism at the highest concentration (100 µg/mL). Soil communities demonstrated resilience up to 100 µg/mL, beyond which there was a significant decrease in population growth and the capacity to metabolize carbohydrates and polymers. Despite microbial mitigation, HQ remains highly toxic to various trophic levels, emphasizing the necessity for environmental regulations.

show abstract

Section: Resultsmentioning

confidence: 99%

“…Interestingly, these derivatives appear to downregulate genes of Aliivibrio spp. implicated in motility, protease synthesis, indol, and capsular polysaccharide production, suggesting a potential mechanism of action [ 72 ].…”

Section: Resultsmentioning

confidence: 99%

Hydroquinone Ecotoxicity: Unveiling Risks in Soil and River Ecosystems with Insights into Microbial Resilience

Valenzuela,

Ballestero,

Gan

et al. 2024

Toxics

View full text Add to dashboard Cite

show abstract

“…Indole production was evaluated in the occurrence of halogenated acid derivatives at various pH ( Sathiyamoorthi et al., 2023a ). In summary, the culture that had grown overnight was dilute using a 1:100 dilution with mLB.…”

Section: Methodsmentioning

confidence: 99%

Antibacterial and antibiofilm activity of halogenated phenylboronic acids against Vibrio parahaemolyticus and Vibrio harveyi

Sathiyamoorthi,

Lee,

Lee

2024

Front. Cell. Infect. Microbiol.

Self Cite

View full text Add to dashboard Cite

Vibrios are associated with live seafood because they are part of the indigenous marine microflora. In Asia, foodborne infections caused by Vibrio spp. are common. In recent years, V. parahaemolyticus has become the leading cause of all reported food poisoning outbreaks. Therefore, the halogenated acid and its 33 derivatives were investigated for their antibacterial efficacy against V. parahaemolyticus. The compounds 3,5-diiodo-2-methoxyphenylboronic acid (DIMPBA) and 2-fluoro-5-iodophenylboronic acid (FIPBA) exhibited antibacterial and antibiofilm activity. DIMPBA and FIPBA had minimum inhibitory concentrations of 100 μg/mL for the planktonic cell growth and prevented biofilm formation in a dose-dependent manner. Both iodo-boric acids could diminish the several virulence factors influencing the motility, agglutination of fimbria, hydrophobicity, and indole synthesis. Consequently, these two active halogenated acids hampered the proliferation of the planktonic and biofilm cells. Moreover, these compounds have the potential to effectively inhibit the presence of biofilm formation on the surface of both squid and shrimp models.

show abstract

“…The potential of IPBC to inhibit Vibrio motility phenotypes was evaluated, as reported previously 29 . Semi-solid mLB plates containing 0.3% agar and IPBC (0, 10, 20, 50, or 100 μg/mL) were used to determine swimming motility.…”

Section: Methodsmentioning

confidence: 99%

Antibacterial and antibiofilm activities of iodinated hydrocarbons against Vibrio parahaemolyticus and Staphylococcus aureus

Faleye,

Lee

et al. 2024

Sci Rep

Self Cite

View full text Add to dashboard Cite

Food-related illnesses have become a growing public concern due to their considerable socioeconomic and medical impacts. Vibrio parahaemolyticus and Staphylococcus aureus have been implicated as causative organisms of food-related infections and poisoning, and both can form biofilms which confer antibiotic resistance. Hence, the need for continuous search for compounds with antibiofilm and antivirulence properties. In this study, 22 iodinated hydrocarbons were screened for their antibiofilm activity, and of these, iodopropynyl butylcarbamate (IPBC) was found to effectively control biofilm formation of both pathogens with a MIC of 50 µg/mL which was bactericidal to V. parahaemolyticus and S. aureus. Microscopic studies confirmed IPBC inhibits biofilm formation of both bacteria and also disrupted their mixed biofilm formation. Furthermore, IPBC suppressed virulence activities such as motility and hemolytic activity of V. parahaemolyticus and the cell surface hydrophobicity of S. aureus. It exhibited a preservative potential against both pathogens in a shrimp model. IPBC disrupted the cell membrane of S. aureus and V. parahaemolyticus and differentially affected gene expressions related to biofilm formation and virulence. Additionally, it displayed broad-spectrum antibiofilm activities against other clinically relevant pathogens. These findings indicate IPBC offers a potential means of controlling infections mediated by Vibrio and Staphylococcus biofilms.

show abstract

Hydroquinone derivatives attenuate biofilm formation and virulence factor production in Vibrio spp

Cited by 9 publications

References 83 publications

Hydroquinone Ecotoxicity: Unveiling Risks in Soil and River Ecosystems with Insights into Microbial Resilience

Hydroquinone Ecotoxicity: Unveiling Risks in Soil and River Ecosystems with Insights into Microbial Resilience

Antibacterial and antibiofilm activity of halogenated phenylboronic acids against Vibrio parahaemolyticus and Vibrio harveyi

Antibacterial and antibiofilm activities of iodinated hydrocarbons against Vibrio parahaemolyticus and Staphylococcus aureus

Contact Info

Product

Resources

About