Hydrosoluble phylloplane components of Theobroma cacao modulate the metabolism of Moniliophthora perniciosa spores during germination

Mares, Joise Hander; Gramacho, Karina Peres; Santana, Juliano Oliveira; Souza, Aurizângela Oliveira de; Alvim, Fátima Cerqueira; Pirovani, Carlos Priminho

doi:10.1016/j.funbio.2019.11.008

Cited by 7 publications

(6 citation statements)

References 28 publications

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“…Proteomic studies of M. perniciosa subjected to treatments with leaf washes and with a PR-10 of Theobroma cacao were found. Basidiospores of M. perniciosa germinated in the presence of leaf washes from contrasting genotypes of Theobroma cacao showed a distinct protein profile [ 114 ]. The authors highlighted the reduced ATP synthase of the germinated basidiospores in the leaf wash of the susceptible genotype, suggesting a shift from aerobic to fermentative metabolism.…”

Section: Discussionmentioning

confidence: 99%

“…The phylloplane is considered the first battleground for cocoa against M. perniciosa , and the resistant genotype CCN51 has an index of short glandular trichomes two times higher than the susceptible genotype, Catongo [ 124 ]. Water-soluble compounds from the phylloplane of Theobroma cacao have demonstrated an inhibitory effect on germination of spores of the fungus [ 114 ]. The proteomics of the phylloplane of the cocoa genotype resistant to WBD revealed proteins related to defense, synthesis of defense metabolites and the metabolism of nucleic acids, demonstrating that proteins and water-soluble compounds secreted to the phylloplane of cocoa participate in the defense against pathogens.…”

Section: Discussionmentioning

confidence: 99%

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State of the Art of the Molecular Biology of the Interaction between Cocoa and Witches’ Broom Disease: A Systematic Review

Santos

Mora-Ocampo

Novais

et al. 2023

IJMS

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Significant scientific advances to elucidate the Moniliophthora perniciosa pathosystem have been achieved in recent years, but the molecular biology of this pathogen-host interaction is still a field with many unanswered questions. In order to present insights at the molecular level, we present the first systematic review on the theme. All told, 1118 studies were extracted from public databases. Of these, 109 were eligible for the review, based on the inclusion and exclusion criteria. The results indicated that understanding the transition from the biotrophic-necrotrophic phase of the fungus is crucial for control of the disease. Proteins with strong biotechnological potential or that can be targets for pathosystem intervention were identified, but studies regarding possible applications are still limited. The studies identified revealed important genes in the M. perniciosa-host interaction and efficient molecular markers in the search for genetic variability and sources of resistance, with Theobroma cacao being the most common host. An arsenal of effectors already identified and not explored in the pathosystem were highlighted. This systematic review contributes to the understanding of the pathosystem at the molecular level, offering new insights and proposing different paths for the development of new strategies to control witches’ broom disease.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

State of the Art of the Molecular Biology of the Interaction between Cocoa and Witches’ Broom Disease: A Systematic Review

Santos

Mora-Ocampo

Novais

et al. 2023

IJMS

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“…Also, alcohol dehydrogenase (ADH) (spot 347) was identified exclusively in ungerminated spores, in addition to its interaction in the protein network, grouped in cluster F and high expression in the transcripts (Figures 6 and 8). Basidiospores of M. perniciosa germinated in cocoa leaf wash and Diplodia seriata grown under carboxymethylcellulose conditions showed an increase in the accumulation of this enzyme, these fungi probably use the ADH fermentation pathway to facilitate obtaining energy (Cobos et al, 2010; Mares et al, 2020). Thus, the detection of proteins involved in the process of obtaining energy, both via glycolysis and fermentation, suggests that the molecular processes necessary to trigger germination may occur within the ungerminated spores of M. roreri .…”

Section: Discussionmentioning

confidence: 99%

Comparative proteome profile of ungerminated spores and mycelium of the fungus Moniliophthora roreri, causal agent of frosty pod rot disease in cacao

Zugaib

Gramacho

Mares

et al. 2023

Journal of Phytopathology

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Moniliasis caused by the fungus Moniliophthora roreri is one of the most destructive cacao diseases in tropical America. In 2021, the presence of the pathogen was confirmed in cacao plantations in Brazil. This is the first proteomic study of the M. roreri protein profile of ungerminated spores and mycelium compared by 2D SDS‐PAGE (Two‐dimensional gel electrophoresis) associated with mass spectrometry. A total of 446 spots were detected on ungerminated spores gels and 402 spots on M. roreri mycelial gels. A total of 29 proteins were identified from the ungerminated spores, 21 of them were exclusive, and 53 were identified in the mycelium, 16 of them were exclusive. Most of the identified proteins at both development stages were categorized as being involved in metabolic processes, reduction/oxidation processes, and protein synthesis and folding. The interaction networks observed expand the known interactions of these proteins, resulting in eight functional clusters for proteins identified in ungerminated spores and seven clusters for mycelial proteins. In addition, a biological model of ungerminated spores and mycelium with cell location of proteins was constructed. The results contribute to a better understanding of the metabolic mechanisms of M. roreri during dormancy and vegetative development, which can support future studies for strategies to control moniliasis.

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“…The most cited papers in the SRDB formed five clusters of co-citations (Figure 2d): cluster 1 contained papers discussing, among other results, the population structure or subpopulation of M. perniciosa and M. roreri pathogens [14,[44][45][46] and Phytophthora spp. [47,48]; cluster 2 contained papers discussing M. perniciosa effector proteins [49][50][51]; cluster 3 contained papers based on molecular markers discussing M. perniciosa genetic diversity, which is similar to cluster 1 [52][53][54]; cluster 4 contained studies characterizing and evaluating M. perniciosa proteins interacting with T. cacao [55][56][57]; and cluster 5 contained molecular responses of M. roreri during its interaction with T. cacao [58,59]. The selected studies were predominantly published in journals related to phytopathology and fungal genetics, as expected.…”

Section: Bibliometric Analysismentioning

confidence: 99%

Genomic and Pathogenicity Mechanisms of the Main Theobroma cacao L. Eukaryotic Pathogens: A Systematic Review

et al. 2023

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A set of diseases caused by fungi and oomycetes are responsible for large losses in annual world cocoa production. Managing the impact caused by these diseases is very complex because a common solution has yet to be found for different pathogens. In this context, the systematic knowledge of Theobroma cacao L. pathogens’ molecular characteristics may help researchers understand the possibilities and limitations of cocoa disease management strategies. This work systematically organized and summarized the main findings of omics studies of T. cacao eukaryotic pathogens, focusing on the plant–pathogen interaction and production dynamics. Using the PRISMA protocol and a semiautomated process, we selected papers from the Scopus and Web of Science databases and collected data from the selected papers. From the initial 3169 studies, 149 were selected. The first author’s affiliations were mostly from two countries, Brazil (55%) and the USA (22%). The most frequent genera were Moniliophthora (105 studies), Phytophthora (59 studies) and Ceratocystis (13 studies). The systematic review database includes papers reporting the whole-genome sequence from six cocoa pathogens and evidence of some necrosis-inducing-like proteins, which are common in T. cacao pathogen genomes. This review contributes to the knowledge about T. cacao diseases, providing an integrated discussion of T. cacao pathogens’ molecular characteristics, common mechanisms of pathogenicity and how this knowledge is produced worldwide.

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Hydrosoluble phylloplane components of Theobroma cacao modulate the metabolism of Moniliophthora perniciosa spores during germination

Cited by 7 publications

References 28 publications

State of the Art of the Molecular Biology of the Interaction between Cocoa and Witches’ Broom Disease: A Systematic Review

State of the Art of the Molecular Biology of the Interaction between Cocoa and Witches’ Broom Disease: A Systematic Review

Comparative proteome profile of ungerminated spores and mycelium of the fungus Moniliophthora roreri, causal agent of frosty pod rot disease in cacao

Genomic and Pathogenicity Mechanisms of the Main Theobroma cacao L. Eukaryotic Pathogens: A Systematic Review

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