Hydrostatic pressure promotes chondrogenic differentiation and microvesicle release from human embryonic and bone marrow stem cells

Luo, Lilin; Foster, Nicola C.; Man, Kenny; Brunet, Mathieu; Hoey, David A.; Cox, Sophie C.; Kimber, Susan J.; Haj, Alicia J. El

doi:10.1002/biot.202100401

Cited by 16 publications

(9 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Studies conducted in the past have indicated that HP at low loads (1–50 kPa) has an osteogenic impact on MSCs, whereas HP at physiological loads efficiently promoted chondrogenic differentiation[ 63 ]. This concept was also supported by several recent research works[ 60 , 64 ]. Some investigations, however, discovered a facilitative effect of physiological loading on MSCs' osteogenic differentiation[ 59 , 65 ], and a chondrogenic effect of low loading on MSCs[ 61 , 62 ].…”

Section: Physical Stimulisupporting

confidence: 59%

Stimulating factors for regulation of osteogenic and chondrogenic differentiation of mesenchymal stem cells

Zhou¹,

Wan²,

Wang³

et al. 2023

World J Stem Cells

View full text Add to dashboard Cite

Mesenchymal stem cells (MSCs), distributed in many tissues in the human body, are multipotent cells capable of differentiating in specific directions. It is usually considered that the differentiation process of MSCs depends on specialized external stimulating factors, including cell signaling pathways, cytokines, and other physical stimuli. Recent findings have revealed other underrated roles in the differentiation process of MSCs, such as material morphology and exosomes. Although relevant achievements have substantially advanced the applicability of MSCs, some of these regulatory mechanisms still need to be better understood. Moreover, limitations such as long-term survival in vivo hinder the clinical application of MSCs therapy. This review article summarizes current knowledge regarding the differentiation patterns of MSCs under specific stimulating factors.

show abstract

Section: Physical Stimulisupporting

confidence: 59%

Stimulating factors for regulation of osteogenic and chondrogenic differentiation of mesenchymal stem cells

Zhou¹,

Wan²,

Wang³

et al. 2023

World J Stem Cells

View full text Add to dashboard Cite

show abstract

“…Due to their unique biological roles, EVs coordinate complex processes, such as development, inflammation, tumorigenesis and even maintenance of a stem cell niche [ 201 ]. EVs release responses to external influences, such as cyclic hydrostatic pressure [ 202 ], oxidative stress [ 203 ] and hypoxia [ 204 ]. Types of EVs are distinguished for subclasses by their fundamental mechanisms of biogenesis, diversity of their composition and size [ 205 ] and selective cargo loading [ 206 , 207 ].…”

Section: Extracellular Vesicles (Evs) As a Tool For Cell Fate Manipul...mentioning

confidence: 99%

Strategies to Convert Cells into Hyaline Cartilage: Magic Spells for Adult Stem Cells

Kurenkova

Романова

Kibirskiy

et al. 2022

IJMS

View full text Add to dashboard Cite

Damaged hyaline cartilage gradually decreases joint function and growing pain significantly reduces the quality of a patient’s life. The clinically approved procedure of autologous chondrocyte implantation (ACI) for treating knee cartilage lesions has several limits, including the absence of healthy articular cartilage tissues for cell isolation and difficulties related to the chondrocyte expansion in vitro. Today, various ACI modifications are being developed using autologous chondrocytes from alternative sources, such as the auricles, nose and ribs. Adult stem cells from different tissues are also of great interest due to their less traumatic material extraction and their innate abilities of active proliferation and chondrogenic differentiation. According to the different adult stem cell types and their origin, various strategies have been proposed for stem cell expansion and initiation of their chondrogenic differentiation. The current review presents the diversity in developing applied techniques based on autologous adult stem cell differentiation to hyaline cartilage tissue and targeted to articular cartilage damage therapy.

show abstract

“…The presence of tetraspanin markers on the surface of EVs was assessed using the ExoView™ Tetraspanin Kit (NanoView Biosciences, United States) according to the manufacturers’ protocol and as previously described ( Gupta et al, 2020 ; Luo et al, 2021 ). Briefly, 35 µL of EV suspension (1:1,000 dilution in Incubation Solution) was incubated on the ExoView™ chip for 16 h. The chip was washed with Incubation Solution for 3 min, three times using Wash Solution and then with deionized water.…”

Section: Methodsmentioning

confidence: 99%

An ECM-Mimetic Hydrogel to Promote the Therapeutic Efficacy of Osteoblast-Derived Extracellular Vesicles for Bone Regeneration

Man

Brunet

Federici

et al. 2022

Front. Bioeng. Biotechnol.

Self Cite

View full text Add to dashboard Cite

The use of extracellular vesicles (EVs) is emerging as a promising acellular approach for bone regeneration, overcoming translational hurdles associated with cell-based therapies. Despite their potential, EVs short half-life following systemic administration hinders their therapeutic efficacy. EVs have been reported to bind to extracellular matrix (ECM) proteins and play an essential role in matrix mineralisation. Chitosan and collagen type I are naturally-derived pro-osteogenic biomaterials, which have been demonstrated to control EV release kinetics. Therefore, this study aimed to develop an injectable ECM-mimetic hydrogel capable of controlling the release of osteoblast-derived EVs to promote bone repair. Pure chitosan hydrogels significantly enhanced compressive modulus (2.48-fold) and osteogenic differentiation (3.07-fold), whilst reducing gelation times (2.09-fold) and proliferation (2.7-fold) compared to pure collagen gels (p ≤ 0.001). EV release was strongly associated with collagen concentration (R2 > 0.94), where a significantly increased EV release profile was observed from chitosan containing gels using the CD63 ELISA (p ≤ 0.001). Hydrogel-released EVs enhanced human bone marrow stromal cells (hBMSCs) proliferation (1.12-fold), migration (2.55-fold), and mineralisation (3.25-fold) compared to untreated cells (p ≤ 0.001). Importantly, EV-functionalised chitosan-collagen composites significantly promoted hBMSCs extracellular matrix mineralisation when compared to the EV-free gels in a dose-dependent manner (p ≤ 0.001). Taken together, these findings demonstrate the development of a pro-osteogenic thermosensitive chitosan-collagen hydrogel capable of enhancing the therapeutic efficacy of osteoblast-derived EVs as a novel acellular tool for bone augmentation strategy.

show abstract

Hydrostatic pressure promotes chondrogenic differentiation and microvesicle release from human embryonic and bone marrow stem cells

Cited by 16 publications

References 66 publications

Stimulating factors for regulation of osteogenic and chondrogenic differentiation of mesenchymal stem cells

Stimulating factors for regulation of osteogenic and chondrogenic differentiation of mesenchymal stem cells

Strategies to Convert Cells into Hyaline Cartilage: Magic Spells for Adult Stem Cells

An ECM-Mimetic Hydrogel to Promote the Therapeutic Efficacy of Osteoblast-Derived Extracellular Vesicles for Bone Regeneration

Contact Info

Product

Resources

About