This mineralogical and fluid inclusion study was conducted on an Au-bearing quartz–sulfide vein encountered in the deep parts of the Bytíz deposit in the Příbram uranium and base-metal district, Bohemian Massif, Czech Republic. The samples were taken where the Au-bearing vein is crosscut by the common base-metal Zn-Pb ore vein Bt23C. The early mineralization of the Au-bearing vein is composed mainly of quartz (Q-1 to Q-3), illite–muscovite, Fe-Mg chlorite, arsenopyrite, and Au-Ag alloys, showing a wide range of compositions (4–69 at. % Ag) and a decrease in Au/(Au + Ag) ratios during vein evolution. Younger hydrothermal processes led to the crystallization of nests and veinlets composed of late quartz (Q-4), carbonates (siderite, dolomite–ankerite and calcite), base-metal sulfides (galena, sphalerite, chalcopyrite, and tetrahedrite), a suite of Ag and Bi-tellurides, and acanthite. The input of Sb is manifested by the partial to complete replacement of some gold grains by aurostibite and an unnamed (Ag,Au)-Sb oxide with a composition close to AuSbO3. The fluid inclusion study, combined with chlorite thermometry and arsenopyrite thermometry, showed that the early mineralization crystallized from progressively cooled (from 300 to 400 °C down to ca. 180 °C), diluted (1.2–7.0 wt. % NaCl eq.) aqueous solutions. The late portion of the mineralization formed from aqueous fluids with highly variable salinity (0.2–23.4 wt. % NaCl eq.) and homogenization temperatures decreasing from ca. 250 °C to < 50 °C, which compare well with the base-metal mineralization of the vein Bt23C and other base-metal veins of the Příbram ore area. Our study illustrates the nature and intensity of the processes of the reworking of the early gold mineralization mediated by the younger Ag,Sb-rich base-metal fluids, giving rise to Příbram’s typical late-Variscan vein Zn-Pb mineralization.