Hydrothermal Assisted Biogenic Synthesis of Silver Nanoparticles And Their Anticandidal Activity On Virulent Candida Isolates From COVID-19 Patients

Abstract

Till now the exact mechanism and effect of biogenic silver nanoparticles on fungus is an indefinable question. To focus on this issue, we prepared hydrothermal assisted thyme coated silver nanoparticles (T/AgNPs) and their toxic effect on Candida isolates is described. The role of thyme (Thymus Vulgaris) in the reduction of silver ions and stabilization of T/AgNPs are estimated by Fourier transforms infrared spectroscopy, structure and size of present silver nanoparticles were detected via atomic force microscopy as well as high-resolution transmission electron microscopy. The biological activity of T/AgNPs was observed against Candida isolates from COVID-19 Patients. Testing of virulence of Candida species using Multiplex PCR. T/AgNPs proved highly effective against Candida albicans, Candida kruzei, Candida glabrata and MIC values ranging from 156.25 to 1,250 µg/mL and MFC values ranging from 312.5 to 5,000 µg/mL. The structural and morphological modifications due to T/AgNPs on Candida albicans were detected by TEM. It was highly observed that when Candida albicans cells were subjected to 50 and 100 µg/mL T/AgNPs, a remarkable change in the cell wall and cell membrane was observed.

Novel Study of the Effect of Bilobetein Compound and Silver Nano Particles of Ginkgo Biloba as Folate Antagonists by Inhibiting Enzyme Dihydrofolate Reductase in Iraqi Patients Serum of Small Cell and Adenocarcinoma Lung Cancer

“…When the extract was being prepared, the hue abruptly changed from translucent green to black, signifying the creation of Ag NPs. AgNPs were powdered, conserved in an airtight bottle, and dried in an oven at 60 °C for 18 hours (14) . Preparation of solutions: Bilobetein and silver nanoparticles of ginkgo biloba herb solutions were synthesized by adding 0.005 mg of each to be dissolved in 100 ml of water to create a stock standard solution.…”