Hydrothermally derived water-dispersible mixed valence copper-chitosan nanocomposite as exceptionally potent antimicrobial agent

Basumallick, Srijita; Rajasekaran, P.; Tetard, Laurene; Santra, Swadeshmukul

doi:10.1007/s11051-014-2675-9

Cited by 17 publications

(17 citation statements)

References 29 publications

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“…Furthermore, highly depolymerized chitosan possesses exceptional muco-adhesive property owing to the presence of high net positive charge provided by the amine group in the linear polysaccharide matrix ( 13 ). We also showed that the increased functional groups after HT treatment help in enhancing the dispersibility of chitosan in water, thus significantly potentiating the usefulness as a feed additive by overcoming the bottleneck of insolubility ( 18 ). By taking advantage of the above-mentioned merits, herein, we developed a chitosan hydrogel matrix that is loaded with both copper and zinc metal nanoparticles that can be used as a potential micronutrient-based antimicrobial feed additive.…”

Section: Introductionmentioning

confidence: 89%

“…One pot HT method of making water-dispersible chitosan–metal composite hydrogel was performed as described earlier ( 18 ). Briefly, low molecular weight chitosan (Sigma-Aldrich, St. Louis, MO, USA) was dissolved in 1% HCl at 10 mg/ml and HT depolymerization was done inside a preheated Lindeberg/Blue M oven (Thermoelectron Corporation) at 150°C for 90 min.…”

Section: Methodsmentioning

confidence: 99%

“…Initial screening for determining the most effective metallic concentration was performed using a microplate alamar blue assay (MABA) as described elsewhere ( 18 ). The MABA assay was used as a substitute for broth microdilution assay to determine minimal inhibitory concentration (MIC) to circumvent the interference of the chitosan hydrogel in light absorbance.…”

Section: Methodsmentioning

confidence: 99%

“…We recently demonstrated that hydrothermal (HT) treatment of chitosan results in depolymerization of chitosan into shorter chain polymers with increased functional groups availability for loading high amounts of antimicrobial copper and similar metals ( 18 ). Furthermore, highly depolymerized chitosan possesses exceptional muco-adhesive property owing to the presence of high net positive charge provided by the amine group in the linear polysaccharide matrix ( 13 ).…”

Section: Introductionmentioning

confidence: 99%

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Hydrothermally Treated Chitosan Hydrogel Loaded with Copper and Zinc Particles as a Potential Micronutrient-Based Antimicrobial Feed Additive

Rajasekaran

Santra

2015

Front. Vet. Sci.

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Large-scale use of antibiotics in food animal farms as growth promoters is considered as one of the driving factors behind increasing incidence of microbial resistance. Several alternatives are under investigation to reduce the amount of total antibiotics used in order to avoid any potential transmission of drug resistant microbes to humans through food chain. Copper sulfate and zinc oxide salts are used as feed supplement as they exhibit antimicrobial properties in addition to being micronutrients. However, higher dosage of copper and zinc (often needed for growth promoting effect) to animals is not advisable because of potential environmental toxicity arising from excreta. Innovative strategies are needed to utilize the complete potential of trace minerals as growth promoting feed supplements. To this end, we describe here the development and preliminary characterization of hydrothermally treated chitosan as a delivery vehicle for copper and zinc nanoparticles that could act as a micronutrient-based antimicrobial feed supplement. Material characterization studies showed that hydrothermal treatment makes a chitosan hydrogel that rearranged to capture the copper and zinc metal particles. Systemic antimicrobial assays showed that this chitosan biopolymer matrix embedded with copper (57.6 μg/ml) and zinc (800 μg/ml) reduced the load of model gut bacteria (target organisms of growth promoting antibiotics), such as Escherichia coli, Enterococcus faecalis, Staphylococcus aureus, and Lactobacillus fermentum under in vitro conditions. Particularly, the chitosan/copper/zinc hydrogel exhibited significantly higher antimicrobial effect against L. fermentum, one of the primary targets of antibiotic growth promoters. Additionally, the chitosan matrix ameliorated the cytotoxicity levels of metal supplements when screened against a murine macrophage cell line RAW 264.7 and in TE-71, a murine thymic epithelial cell line. In this proof-of-concept study, we show that by using chitosan as a delivery platform, micronutrient-based metal feed additives could be used to minimize the undesirable levels of microbial population without causing significant cytotoxic effect under in vitro conditions. These findings provide the platform for further studies in target animal models to quantify the required physiological concentrations of copper and zinc when delivered via a chitosan hydrogel platform to elicit a growth promoting effect without causing any toxicity.

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Section: Introductionmentioning

confidence: 89%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Hydrothermally Treated Chitosan Hydrogel Loaded with Copper and Zinc Particles as a Potential Micronutrient-Based Antimicrobial Feed Additive

Rajasekaran

Santra

2015

Front. Vet. Sci.

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“…Most of the reported antimicrobial studies involving MNPs have so far mainly been confined to the use of silver (Azocar et al 2014;Chudasama et al 2010;Kahrilas et al 2014;Martinez-Castanon et al 2008;Panacek et al 2006;Ferrer et al 2012;Song et al 2012;Panacek et al 2009), few on copper (Basumallick et al 2014;Chatterjee et al 2012;Cioffi et al 2004Cioffi et al , 2005Wei et al 2010), and least on gold (Moreno-Alvarez et al 2010;Zhao et al 2010); however, the cost and the availability of Ag pose a major hindrance to its applications. In this pursuit, nanoparticles (NPs) of Cu (the other coinage metal apart from Au and Ag) have been realized as a viable alternative, keeping in mind that people in ancient times used containers made of Cu and Cu-alloys for preserving water and food, the main transmitters of pathogen for most microorganisms borne diseases.…”

Section: Introductionmentioning

confidence: 99%

Carboxymethylated chitosan-stabilized copper nanoparticles: a promise to contribute a potent antifungal and antibacterial agent

et al. 2015

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Carboxymethylated chitosan (CMC)-stabilized copper nanoparticles (Cu-NPs) have been synthesized via chemical reduction of copper(II)-CMC complex in aqueous medium by hydrazine under microwave irradiation in ambient atmosphere. Structural morphology, phase, and chemical compositions of CMC-stabilized Cu-NPs (CMC-Cu-NPs) have been analyzed through high-resolution transmission electron microscopy, field emission scanning electron microscopy, X-ray diffraction, and X-ray photoelectron spectroscopy. Antifungal and antibacterial activities of CMC-Cu-NPs have been evaluated against Candida tropicalis and Escherichia coli through agar well diffusion method, broth microdilution assay, live-dead assay, and microscopic observation. Antimicrobial activity of spherical CMC-Cu-NPs (*4-15 nm of diameters) has been observed to be significant for both C. tropicalis and E. coli. The cytotoxicity study indicates that CMC-Cu-NPs have no significant toxic effect against normal cell line, L929.

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Chitosan-Based Nanocomposites as Remarkably Effectual Wound Healing Agents

Vijayan

Gulati

Sahu

et al. 2022

Chitosan-Based Nanocomposite Materials

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Hydrothermally derived water-dispersible mixed valence copper-chitosan nanocomposite as exceptionally potent antimicrobial agent

Cited by 17 publications

References 29 publications

Hydrothermally Treated Chitosan Hydrogel Loaded with Copper and Zinc Particles as a Potential Micronutrient-Based Antimicrobial Feed Additive

Hydrothermally Treated Chitosan Hydrogel Loaded with Copper and Zinc Particles as a Potential Micronutrient-Based Antimicrobial Feed Additive

Carboxymethylated chitosan-stabilized copper nanoparticles: a promise to contribute a potent antifungal and antibacterial agent

Chitosan-Based Nanocomposites as Remarkably Effectual Wound Healing Agents

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