analyses. 1-and 2-dimensional NMR spectroscopy of the rare naringenin 5-O-glucoside have been recorded and assigned for the first time. The flavonoid glucosides from Chrysanthemum coronarium showed week activity against Poliovirus I and Adenovirus type 7.Chrysanthemum coronarium L. (Compositae) is regarded in East Asia as a healthy vegetable because the edible portion contains abundant nutrients [1] as well as a fresh flavor [2]. This species is occasionally used as folk medicine [3] and has been found to have biologically active substances [4][5][6][7]. The elucidation of the bioactive substances from this plant is of a great importance to increase its vegetable value. It is used in Egyptian traditional medicine [8]. It was reported that the plant contained terpenes [4], sterols, polyacetylenes [6], anthraquinones [9], and flavonoids [9,10]. In the course of our continuous studies on this plant, naringenin 5-O-glucoside (1) [11, 12] as a rare flavanone glucoside was isolated with two flavone glucosides, apigenin 7-O-β-glucoside (2) and luteolin 7-O-β-glucoside (3); two aglycones, apigenin (4) and luteolin (5); two flavonol glucosides, kaempferol 3-O-β-glucoside (6) and quercetin 3-O-β-glucoside (isoquercitrin) (7) [11,13]; and two aglycones, kaempferol (8) and quercetin (9). 2: R = R 2 = H, R 1 = Glc; 3: R = OH, R 1 = Glc, R 2 = H 4: R = R 1 = R 2 = H; 5: R = OH, R 1 = R 2 = H 6: R = R 1 = H, R 2 = OGlc; 7: R 1 = H, R = OH, R 2 = OGlc 8: R = R 1 = H, R 2 = OH; 9: R = OH, R 1 = H, R 2 = OH Ground dry shrublets of C. coronarium were exhaustively extracted with 70% ethanol/water, and the extract was concentrated under reduced pressure. The aqueous residue was extracted with solvents of increasing polarity. The fractions were subjected to repeated column chromatography to yield nine compounds.