Hydroxyapatite cages with aligned pores for bone grafting – Seeding of human osteoblast-like cells in vitro and their response in dynamic culture mode

Athira, R.K.; G, Gayathry; Kumar, P.R. Anil; Varma, P. R. Harikrishna; Kasoju, Naresh; Komath, Manoj

doi:10.1016/j.ceramint.2021.07.181

Cited by 4 publications

(7 citation statements)

References 40 publications

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“…Once the self‐replicating and trilineage differentiation capabilities of hWJ‐MSCs under standard conditions were confirmed, the first aim was to investigate the attachment and viability of hWJ‐MSCs on HA scaffolds. For this purpose, the axially aligned porous HA scaffolds were prepared as per an earlier report 21 . Subsequently, hWJ‐MSCs were seeded onto the bioceramic scaffolds, they were then clipped onto the inserts, assembled inside a multiwell plate and cultured for 24 h in dynamic conditions in the bioreactor setup.…”

Section: Resultsmentioning

confidence: 99%

“…For this purpose, the axially aligned porous HA scaffolds were prepared as per an earlier report. 21 Subsequently, hWJ-MSCs were seeded onto the bioceramic scaffolds, they were then clipped onto the inserts, assembled inside a multiwell plate and cultured for 24 h in dynamic conditions in the bioreactor setup. As evident from the light microscopy, lumps of cells were noted over the F I G U R E 2 Morphological and phenotypical changes in hWJ-MSCs cultured in PLM and ODM: cells in PLM showed characteristic fibroblast-like morphology both on Days 1 and 21, and stained negative for Az red.…”

Section: Hwj-msc Adhesion and Viability Over Bioceramic Scaffoldsmentioning

confidence: 99%

“…The preparation process of the HA ceramic grafts with aligned pore structures has been described elsewhere. 21 Essentially it follows the steps of preparation of HA slips, casting them in molds with linear pore formers (fibers of 250 µm diameter) and subjecting the green body to sintering. The HA powder was prepared by wet precipitation reaction in an ammoniated solution comprising calcium nitrate tetrahydrate and ammonium dihydrogen orthophosphate.…”

Section: Preparation Of Ha Ceramics With Aligned Poresmentioning

confidence: 99%

“…A custom‐developed multiwell format bioreactor setup was used to simulate the dynamic culture conditions. The study is a continuation of an earlier published work that demonstrated the design, fabrication, and characterization of aligned porous HA scaffolds, and preliminary cell culture studies with model cell lines such as HOS 21 . In the present work, MSCs were selected to simulate the host site environment.…”

Section: Introductionmentioning

confidence: 99%

“…The study is a continuation of an earlier published work that demonstrated the design, fabrication, and characterization of aligned porous HA scaffolds, and preliminary cell culture studies with model cell lines such as HOS. 21 In the present work, MSCs were selected to simulate the host site environment. It is anticipated that the study would pave way for evolving an appropriate in vitro maturation strategy for developing clinically compliant engineered bone tissue grafts.…”

Section: Introductionmentioning

confidence: 99%

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Mesenchymal stem cell culture in aligned porous hydroxyapatite scaffolds using a multiwell plate bioreactor for bone tissue engineering

Athira

et al. 2022

MedComm – Future Medicine

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Regeneration of bone lost by trauma, diseases and aging, and restoration of its load‐bearing function are major clinical challenges. Hydroxyapatite (HA) is a clinically proven scaffold material for bone grafting, but the random‐pore structure limits the homing of the cells inside the graft and the bone regeneration progresses with the resorption of the graft material. This work is based on the hypothesis that aligned through pores in the graft will lead to a faster healing by homing the local cells inside and provide a better environment for new bone formation through the graft structure. The investigation was done using aligned porous HA scaffolds seeded with human Wharton's jelly‐derived mesenchymal stem cells (hWJ‐MSCs) and cultured in a multiwell format bioreactor setup. The cell adhesion was studied by microscopy, cell proliferation was evaluated by Alamar blue assay and osteogenic differentiation was confirmed by biochemical and molecular assays. The results indicate that the hWJ‐MSCs infiltrated through the aligned porous network of the scaffold, proliferated well when cultured in the expansion medium, and differentiated into osteogenic lineage when cultured in the differentiation medium.

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Section: Resultsmentioning

confidence: 99%

Section: Hwj-msc Adhesion and Viability Over Bioceramic Scaffoldsmentioning

confidence: 99%

Section: Preparation Of Ha Ceramics With Aligned Poresmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Mesenchymal stem cell culture in aligned porous hydroxyapatite scaffolds using a multiwell plate bioreactor for bone tissue engineering

Athira

et al. 2022

MedComm – Future Medicine

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Development of the third generation of bioceramics: Doping hydroxyapatite with s-, p-, d-, and f-blocks cations and their potential applications in bone regeneration and void filling

Basirun

Yeong

Thein

2023

Ceramics International

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Potential Load-Bearing Bone Substitution Material: Carbon-Fiber-Reinforced Magnesium-Doped Hydroxyapatite Composites with Excellent Mechanical Performance and Tailored Biological Properties

Zhao

Yang

Liu

et al. 2022

ACS Biomater. Sci. Eng.

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A potential load-bearing bone substitution and repair material, that is, carbon fiber (CF)-reinforced magnesium-doped hydroxyapatite (CF/Mg-HAs) composites with excellent mechanical performance and tailored biological properties, was constructed via the hydrothermal method and spark plasma sintering. A high-resolution transmission electron microscopy (TEM) was employed to characterize the nanostructure of magnesium-doped hydroxyapatite (Mg-HA). TEM images showed that the doping of Mg-induced distortions and dislocations in the hydroxyapatite lattice, resulting in decreased crystallinity and enhanced dissolution. Compressive strengths of 10% magnesium-doped hydroxyapatite (1Mg-HAs) and CF-reinforced 1Mg-HAs (CF/1Mg-HAs) were within the range of that of cortical bone. Compared with 1Mg-HAs, the fracture toughness of CF/1Mg-HAs increased by approximately 38%. The bioactivity, biocompatibility, and osteogenic induction properties of Mg-HAs and CF/Mg-HAs composites were evaluated in vitro using simulated body fluid (SBF) immersion, cell culture, osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), and expression of genes associated with osteogenesis. When Mg-HAs were immersed in SBF, Mg2+ continued to release for up to 21 days. Mg-HAs demonstrated a satisfactory ability to induce apatite formation in comparison with HAs. The cell proliferation and morphology on CF/1Mg-HAs were similar to those of 1Mg-HAs, suggesting that adding CF had no adverse effect on cellular activity. The expression levels of osteogenesis-related genes [osteocalcin (OPN), osteopontin (OCN), and runt-related transcription factor 2 (Runx2)] on 1Mg-HAs were significantly higher at days 3 and 7 than those on HAs and 0.5Mg-HAs groups. This finding suggests that a certain amount of Mg doping had beneficial influences in the different stages of osteogenic differentiation and could induce osteogenic differentiation of BMSCs. The new bone volume to total volume ratio of implanted 1Mg-HAs (30.9% ± 4.1%) and CF/1Mg-HAs (25.4% ± 5.4%) was remarkably higher than that of HAs (21.6% ± 3.9%). 1Mg-HAs and CF/1Mg-HAs tailored an ideal effect of new bone information and implant osseointegration. The excellent mechanical performance and tailored biological properties of CF/Mg-HAs were attributed to nano Mg-doped HA, CF reinforcing, refined microstructure, and controlled composition.

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Hydroxyapatite cages with aligned pores for bone grafting – Seeding of human osteoblast-like cells in vitro and their response in dynamic culture mode

Cited by 4 publications

References 40 publications

Mesenchymal stem cell culture in aligned porous hydroxyapatite scaffolds using a multiwell plate bioreactor for bone tissue engineering

Mesenchymal stem cell culture in aligned porous hydroxyapatite scaffolds using a multiwell plate bioreactor for bone tissue engineering

Development of the third generation of bioceramics: Doping hydroxyapatite with s-, p-, d-, and f-blocks cations and their potential applications in bone regeneration and void filling

Potential Load-Bearing Bone Substitution Material: Carbon-Fiber-Reinforced Magnesium-Doped Hydroxyapatite Composites with Excellent Mechanical Performance and Tailored Biological Properties

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