Hydroxyapatite coating of cellulose sponges attracts bone-marrow-derived stem cells in rat subcutaneous tissue

Tommila, Miretta; Jokilammi, Anne; Terho, Perttu; Wilson, Timothy M.; Penttinen, Risto; Ekholm, Erika

doi:10.1098/rsif.2009.0020

Cited by 21 publications

(11 citation statements)

References 44 publications

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“…540) shows to favor a faster bone repair, evident by micro CT analysis and by the formation of osteoid tissue. Previous studies have indeed shown that elevated Ca 2+ levels could promote the recruitment of bone marrow progenitor cells in vivo [26,27], regulate both bone morphogenetic protein and type I collagen synthesis by osteoblastic cells [28] and modulate the osteoinduction of mesenchymal stem cells [29]. Additionally, we and others have shown that bioactive glasses sustain the expression of proangiogenic cytokines and indirectly induce angiogenesis in vitro [25,30] and in vivo [10].…”

Section: Discussionmentioning

confidence: 65%

The proangiogenic potential of a novel calcium releasing composite biomaterial: Orthotopic in vivo evaluation

et al. 2017

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In current bone tissue engineering approaches the achievement of sufficient angiogenesis, during tissue regeneration, is a major limitation in order to attain full tissue functionality. Recently, we have shown that calcium ions, released by the degradation of calcium phosphate ormoglasses (CaP), are effective angiogenic promoters, in both in vitro and in a subcutaneous implantation model. Here, we devised an injectable composite, containing CaP glass-ceramic particles, dispersed within a HPMC matrix, enabling the release of calcium in a more sustained fashion. We show that by tuning the release of calcium in vivo, in a rat bone defect model, we could improve both bone formation and increase angiogenesis. This simple and cost effective approach holds great promise to translate to the clinics.

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Section: Discussionmentioning

confidence: 65%

The proangiogenic potential of a novel calcium releasing composite biomaterial: Orthotopic in vivo evaluation

et al. 2017

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“…However, despite its potential use as a biomedical scaffold, cellulose has not been widely applied in the eld of hard tissue regeneration owing to a loss of osteoconductivity. 19,20 To overcome the shortcoming of osteogenesis for pure cellulose, various composites using bioceramics (hydroxyapatite and calcium phosphate) have been accommodated. 21,22 In terms of structural formation, pure cellulose cannot be used to directly fabricate a 3D structure consisting of microstruts using a 3D printer because it is easily degraded or decomposed before melting takes place.…”

Section: Introductionmentioning

confidence: 99%

Biomimetic cellulose/calcium-deficient-hydroxyapatite composite scaffolds fabricated using an electric field for bone tissue engineering

et al. 2018

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Cellulose has been widely used as micro/nanofibers in various applications of tissue regeneration, but has certain limitations for bone regeneration, e.g., low biocompatibility in inducing osteogenesis. In addition, the low processability from the decomposition property before melting can be a significant obstacle to fabricating a required complex structure through a 3D-printing process. Herein, to overcome the low osteogenic activity of pure cellulose, we suggest a new cellulose-based composite scaffold consisting of cellulose and a high weight fraction (70 wt%) of calcium-deficient-hydroxyapatite (CDHA), which was obtained from the hydrolysis of a-tricalcium phosphate. Using biocompatible components, we fabricated a 3D pore-structure controllable composite scaffold consisting of microfibrous bundles through an electrohydrodynamic printing (EHDP) process supplemented with an ethanol bath. To obtain a mechanically stable and repeatable 3D mesh structure, various process parameters (nozzle-to-target distance, electric field strength, flow rate, and nozzle moving speed) were considered. As a control, a mesh structure fabricated using a normal EHDP process and with a similar pore geometry was used. A variety of cellular responses using preosteoblasts (MC3T3-E1) indicate that a CDHA/cellulose composite scaffold provides an efficient platform for inducing significantly high bone mineralization.

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“…Tissue-specific CaSR deficient mice exhibit grave defects in bone and cartilage, highlighting the importance of this poorly studied receptor in many physiological processes (Chang et al, 2008). In vivo experiments indicate that bone marrow progenitor cells are mobilised to sites of high calcium concentration -such as calcium releasing biomaterials -possibly by CaSR-mediated chemotaxis, suggesting a role of these cells in high extracellular calcium environments like resorbing bone (Adams et al, 2006;Tommila et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Control of microenvironmental cues with a smart biomaterial composite promotes endothelial progenitor cell angiogenesis

Aguirre¹,

Gonzalez²,

Navarro³

et al. 2012

eCM

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Smart biomaterials play a key role when aiming at successful tissue repair by means of regenerative medicine approaches, and are expected to contain chemical as well as mechanical cues that will guide the regenerative process. Recent advances in the understanding of stem cell biology and mechanosensing have shed new light onto the importance of the local microenvironment in determining cell fate. Herein we report the biological properties of a bioactive, biodegradable calcium phosphate glass/polylactic acid composite biomaterial that promotes bone marrowderived endothelial progenitor cell (EPC) mobilisation, differentiation and angiogenesis through the creation of a controlled bone healing-like microenvironment. The angiogenic response is triggered by biochemical and mechanical cues provided by the composite, which activate two synergistic cell signalling pathways: a biochemical one mediated by the calcium-sensing receptor and a mechanosensitive one regulated by non-muscle myosin II contraction. Together, these signals promote a synergistic response by activating EPCs-mediated VEGF and VEGFR-2 synthesis, which in turn promote progenitor cell homing, differentiation and tubulogenesis. These findings highlight the importance of controlling microenvironmental cues for stem/progenitor cell tissue engineering and offer exciting new therapeutical opportunities for biomaterialbased vascularisation approaches and clinical applications.

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Hydroxyapatite coating of cellulose sponges attracts bone-marrow-derived stem cells in rat subcutaneous tissue

Cited by 21 publications

References 44 publications

The proangiogenic potential of a novel calcium releasing composite biomaterial: Orthotopic in vivo evaluation

The proangiogenic potential of a novel calcium releasing composite biomaterial: Orthotopic in vivo evaluation

Biomimetic cellulose/calcium-deficient-hydroxyapatite composite scaffolds fabricated using an electric field for bone tissue engineering

Control of microenvironmental cues with a smart biomaterial composite promotes endothelial progenitor cell angiogenesis

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