Hydroxyapatite–collagen augments osteogenic differentiation of dental pulp stem cells

Trivedi, Shilpa; Srivastava, Kirti; Saluja, Tajindra Singh; Shyam, Hari; Kumar, Sumit; Singh, Anjana; Saxena, Shailendra K.; Mehrotra, Divya; Singh, Satyendra Kumar

doi:10.1007/s10266-019-00464-0

Cited by 17 publications

(13 citation statements)

References 24 publications

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“…For the effective application of the peptide, a 3D scaffold that properly releases the peptide to ensure that it can migrate toward and attach to target cells and that serves as a substrate for cell proliferation and differentiation (i.e., a drug delivery system) is required [27]. Therefore, the synthesized DPP-derived RGD-containing peptide can be seeded on scaffolds such as collagen (as the major dentin matrix protein), hydroxyapatite (as the main inorganic component of dentin) and polylactic acid (as an artificial biodegradable material) [28][29][30]. By further development of effective application measures of this DPPderived RGD-containing peptide, it is highly likely to regenerate the entire volume of the dentin lost by dental caries because of its biodegradable property.…”

Section: Discussionmentioning

confidence: 99%

Dentin Phosphophoryn-Derived Peptide Promotes Odontoblast Differentiation In Vitro and Dentin Regeneration In Vivo

et al. 2021

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The purpose of the present study was to investigate the effect of a peptide (i.e., SESDNNSSSRGDASYNSDES) derived from dentin phosphophoryn (DPP) with arginine-glycine-aspartic acid (RGD) motifs on odontoblast differentiation in vitro and to compare it with calcium hydroxide—a material used conventionally for vital pulp therapy—in terms of reparative dentin formation and pulp inflammation in vivo. Alkaline phosphatase activity assay and alizarin red S staining were performed to evaluate odontoblast-differentiation in cell culturing experiments. To observe the reparative dentin formation and pulp inflammation animal experiment was performed and examined by histological methods. The difference between the experimental group and the control group was analyzed statistically using a one-way ANOVA test. The results revealed that the DPP-derived RGD-containing peptide triggered odontoblast differentiation and mineralization in vitro. In rats undergoing direct pulp capping, the DPP-derived RGD-containing peptide was found to induce intensively formed reparative dentin with high compactness at week 4. On histological and morphometrical examinations, a smaller degree of pulpitis was observed in the specimens treated with the peptide than in those treated with calcium hydroxide. This study suggests that the DPP-derived RGD-containing peptide is a biocompatible, biodegradable and bioactive material for dentin regeneration.

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Section: Discussionmentioning

confidence: 99%

Dentin Phosphophoryn-Derived Peptide Promotes Odontoblast Differentiation In Vitro and Dentin Regeneration In Vivo

et al. 2021

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“…According to the results of a sulforhodamine colorimetric assay, an alkaline phosphatase assay, and scanning electron microscopy (SEM), the HA-Col scaffold supported DPSC attachment and created a microenvironment that augmented the differentiation of DPSCs into osteogenic cells. Therefore, the researchers suggested that the HA-Col scaffold may represent an optimal material for alveolar bone defect reconstruction [94].…”

Section: Scaffolds Suitable For Alveolar Bone Regenerationmentioning

confidence: 99%

Stem Cells and Their Derivatives—Implications for Alveolar Bone Regeneration: A Comprehensive Review

Hollý

Klein

Mazreku

et al. 2021

IJMS

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Oral and craniofacial bone defects caused by congenital disease or trauma are widespread. In the case of severe alveolar bone defect, autologous bone grafting has been considered a “gold standard”; however, the procedure has several disadvantages, including limited supply, resorption, donor site morbidity, deformity, infection, and bone graft rejection. In the last few decades, bone tissue engineering combined with stem cell-based therapy may represent a possible alternative to current bone augmentation techniques. The number of studies investigating different cell-based bone tissue engineering methods to reconstruct alveolar bone damage is rapidly rising. As an interdisciplinary field, bone tissue engineering combines the use of osteogenic cells (stem cells/progenitor cells), bioactive molecules, and biocompatible scaffolds, whereas stem cells play a pivotal role. Therefore, our work highlights the osteogenic potential of various dental tissue-derived stem cells and induced pluripotent stem cells (iPSCs), the progress in differentiation techniques of iPSCs into osteoprogenitor cells, and the efforts that have been made to fabricate the most suitable and biocompatible scaffold material with osteoinductive properties for successful bone graft generation. Moreover, we discuss the application of stem cell-derived exosomes as a compelling new form of “stem-cell free” therapy.

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“…This method can not only be used as an effective alternative for the treatment of kidney diseases, but also shows the potential application of PLGA scaffold in biomedical fields such as tissue engineering biomimetic scaffolds and biodegradable implants. Shilpa et al [50], through the proliferation and osteogenesis of dental pulp stem cells on hydroxyapatite-collagen (HA-Col) scaffold, proved that the scaffold has no cytotoxicity, and has good biocompatibility, easy adhesion and proliferation of mesenchymal stem cells in vitro, and can enhance the proliferation and osteogenesis of DPSC, is an ideal material for maxillofacial and alveolar bone regeneration. Wu et al [51] made heparinized acellular porcine liver scaffold by terminal attachment technology, which can bind and slowly release heparin binding growth factors.…”

Section: Scaffold Materialsmentioning

confidence: 99%

Research Progress on Tissue Engineering of Main Tissues and Organs of Human Body

Jin

2021

E3S Web Conf.

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The injury and failure diseases of human tissues and organs, such as heart failure and chronic kidney disease, seriously threaten human health and life safety. At present, however, organ transplantation has obvious limitations, and tissue engineering is considered as a potential alternative therapy. Tissue engineering uses the construction of cells, biomaterials and bioreactors to develop three-dimensional artificial tissues and organs for the enhancement, repair and replacement of damaged or diseased tissues and organs, which contributes to the fundamental solutions of diseases of tissues and organs as well as to the improvement of human health. This paper introduces the research progress of tissue engineering technology in the field of living organs from three aspects: seed cells, application of growth factors and biomimetic preparation of functionalized scaffold materials, hoping to provide help and ideas for the research and industrial development of the repair and reconstruction of human organs.

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Hydroxyapatite–collagen augments osteogenic differentiation of dental pulp stem cells

Cited by 17 publications

References 24 publications

Dentin Phosphophoryn-Derived Peptide Promotes Odontoblast Differentiation In Vitro and Dentin Regeneration In Vivo

Dentin Phosphophoryn-Derived Peptide Promotes Odontoblast Differentiation In Vitro and Dentin Regeneration In Vivo

Stem Cells and Their Derivatives—Implications for Alveolar Bone Regeneration: A Comprehensive Review

Research Progress on Tissue Engineering of Main Tissues and Organs of Human Body

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