Hydroxyapatite in the Pathogenesis of Cystic Fibrosis

Warton, Kathryn L.; Blomfield, Jeanette

doi:10.1136/bmj.3.5774.570

Cited by 11 publications

(7 citation statements)

References 14 publications

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“…Although the present study clearly demonstrates that aggregation of CaPP can proceed in the absence of inorganic phosphate, an additional interaction of CaPP with insoluble calcium-phosphate complexes [31] cannot be excluded. Additional studies will hopefully clarify the role, if any, of inorganic phosphate in CaPP aggregation.…”

Section: Discussionmentioning

confidence: 72%

“…turbid SMS has not been completely elucidated. StudOther recent work suggests that inorganic calciumpl~osphorus complexes are also present in the solid phase of turbid SMS [31]. T h e relation between these complexes and the calcium-precipitable protein (CaPP) is unclear.…”

Section: Introductionmentioning

confidence: 99%

“…CaPP was purified from a 600-ml pool of stimulated SMS, collected over a 30-h period from four normal donors, male and female, ages [20][21][22][23][24][25][26][27][28][29][30][31][32][33][34]. Additional samples of CaPP were purified from 30-100 ml of saliva, collected from six patients with CF (ages [14][15][16][17][18][19][20][21][22][23][24][25][26] and six normal subjects (ages [18][19][20][21][22][23][24][25][26][27][28][29][30].…”

Section: Saliva Collectionsmentioning

confidence: 99%

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Purification and Properties of the Calcium-precipitable Protein in Submaxillary Saliva of Normal and Cystic Fibrosis Subjects

1974

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ExtractA calcium-precipitable protein (CaPP), previously shown to cause turbidity of submaxillary saliva (SMS) in patients with cystic fibrosis (CF), was purified from normal and CF-SMS by (NH4) 2 S 0 4 precipitation, gel filtration, and preparative polyacrylamide gel electrophoresis. This protein has a molecular weight of 12,000. I t contains large numbers of serine, glycine, and glutamic acid residues, 0.85y0 phosphorus as phosphate monoester, and small amounts of carbohydrate. Precipitation (aggregation) of purified CaPP occurs at basic pH values in the presence of ionic calcium and is enhanced by increasing calcium concentration, CaPP concentration, or pH. Aggregated CaPP binds calcium in molar quantities equal to the number of phosphate prosthetic groups. Alkaline phosphatase removes all phosphate from CaPP and eliminates calcium-induced precipitation. Studies of CaPP from CF saliva have demonstrated no electrophoretic or compositional differences from normal CaPP. Higher than normal pH values or elevated CaPP concentrations are not present in SMS of patients with CF and do not contribute to the formation of turbid SMS.

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Section: Discussionmentioning

confidence: 72%

Section: Introductionmentioning

confidence: 99%

Section: Saliva Collectionsmentioning

confidence: 99%

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Purification and Properties of the Calcium-precipitable Protein in Submaxillary Saliva of Normal and Cystic Fibrosis Subjects

1974

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“…This proposal is at variance with the previous concept that band 4 protein was precipitated by the high calcium concentration, and that the resultant calciumphosphoprotein aggregates were responsible for the turbidity of the submandibular saliva (Gugler et al, 1967;Boat, 1970), and with the suggestion that mucous obstruction in cystic fibrosis may be due to the presence of highly polymerized units of glycoprotein and hydroxyapatite (Warton and Blomfield, 1971). …”

Section: Discussionmentioning

confidence: 66%

“…The submandibular saliva is turbid compared with the clear saliva of controls, and has raised concentrations of calcium, protein, and the enzymes amylase and acid and alkaline ribonucleases (Chernick, Barbero, and Parkins, 1961;Chernick, Eichel, and Barbero, 1964). Because of the elevated calcium level the solubility limit of (Ca2+) x (HP042-) is exceeded, and calcium is precipitated as hydroxyapatite (Warton and Blomfield, 1971).…”

mentioning

confidence: 99%