Hydroxyapatite nanoparticles

Maia, Ana Luiza Chaves; Cavalcante, Carolina Henriques; Souza, Marina G.F. de; Ferreira, Carolina de Aguiar; Rubello, Domenico; Chondrogiannis, Sotirios; Cardoso, Valbert Nascimento; Ramaldes, Gilson Andrade; Barros, André Luís Branco de; Soares, Daniel Crístian Ferreira

doi:10.1097/mnm.0000000000000510

Cited by 20 publications

(6 citation statements)

References 39 publications

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“…The agglomerates that conform the HA particles may present high rugosity that in turn results in high superficial area values. The specific surface area values found in our study were in the order of the values reported in the literature, which included a wide range from 23.27 m 2 /g [34] to 103.05 m 2 /g [35].…”

Section: Discussionsupporting

confidence: 71%

Development, Characterization and In Vitro Biological Properties of Scaffolds Fabricated From Calcium Phosphate Nanoparticles

Morejón

Delgado

Ribeiro

et al. 2019

IJMS

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Ceramic materials mimic the mineral composition of native bone and feature osteoconductive properties; they are therefore used to regenerate bone tissue. Much research focuses on increasing the porosity and pore interconnectivity of ceramic scaffolds to increase osteoconductivity, cell migration and cell-cell interaction. We aimed to fabricate biocompatible 3D-scaffolds featuring macro- and microporous calcium phosphates with high pore interconnection. Nanoparticles of hydroxyapatite (HA) and calcium deficient hydroxyapatite (CDHA) were synthesized by wet chemical precipitation. Scaffolds were produced from them by the replication polymeric foam technique. Solid content and sintering temperature were varied. Nanoparticles and scaffolds were characterized regarding morphology, chemical and mineral composition, porosity and mechanical properties. Biocompatibility, cell attachment and distribution were evaluated in vitro with human adipose mesenchymal stem cells. Scaffolds with total porosity of 71%–87%, pores in the range of 280–550 µm and connectivity density up to 43 mm−3 were obtained. Smaller pore sizes were obtained at higher sintering temperature. High solid content resulted in a decrease of total porosity but increased interconnectivity. Scaffolds 50HA/50β-TCP featured superior interconnectivity and mechanical properties. They were bioactive and biocompatible. High HA solid content (40 wt.%) in the HA pure scaffolds was negative for cell viability and proliferation, while in the 50HA/50β-TCP composite scaffolds it resulted more biocompatible.

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Section: Discussionsupporting

confidence: 71%

Development, Characterization and In Vitro Biological Properties of Scaffolds Fabricated From Calcium Phosphate Nanoparticles

Morejón

Delgado

Ribeiro

et al. 2019

IJMS

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“…Such low surface charge gives HAP an advantage over the systems that rely on high electrostatic charge, especially if positive, to permeate the plasma membrane and enter the cytoplasm, the reason being the proneness of the latter types of systems to react with the first oppositely charged barriers that they encounter en route to the target tissue while traveling through the vasculature. 35 Although moderate aggregation of nanoparticles in suspension is not ideal, the 300–500 nm hydrodynamic diameter range of HAP/SPION hybrids (Table 3) is adequate for parenteral application, 36 given that this submicron size range is still considerably lower than the 5–10 μ m diameter of the smallest capillary or the dimensions of the discoid erythrocytes: 7 × 1.7 μ m on average. Intravenously administered formulations containing HAP particles with 20–60 μ m in size were used earlier without any adverse effects.…”

Section: Resultsmentioning

confidence: 99%

“…In vivo experiments will be also needed to assess if there are pharmacokinetic benefits of the HAP component compared to the fast clearance and low blood circulation half-life of pure SPIONs: 10 and 90 min for t α 1/2 and t β 1/2 , respectively. 102 Although HAP microparticles did not exhibit any significantly longer pharmacokinetic half-life than SPIONs, their blood residence time was a bit longer, 36 which may be beneficial since the prolonged presence in the bloodstream increases the chances of reaching the tumorous tissue. HAP particles injected intravenously also inhibit platelet aggregation and the coagulation cascade, 103 which is an effect that can indirectly favor tumor targeting via the enhanced permeability and retention (EPR) effect.…”

Section: Discussionmentioning

confidence: 99%

Hydroxyapatite as a Vehicle for the Selective Effect of Superparamagnetic Iron Oxide Nanoparticles against Human Glioblastoma Cells

Pernal

Uskoković

2017

ACS Appl. Mater. Interfaces

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Despite the early promises of magnetic hyperthermia (MH) as a method for treating cancer, it has been stagnating in the past decade. Some of the reasons for the low effectiveness of superparamagnetic nanoparticles (SPIONs) in MH treatments include (a) low uptake in cancer cells; (b) generation of reactive oxygen species that cause harm to the healthy cells; (c) undeveloped targeting potential; and (d) lack of temperature sensitivity between cancer cells and healthy cells. Here we show that healthy cells, including human mesenchymal stem cells (MSCs) and primary mouse kidney and lung fibroblasts, display an unfavorably increased uptake of SPIONs compared to human brain cancer cells (E297 and U87) and mouse osteosarcomas cells (K7M2). Hydroxyapatite (HAP), the mineral component of our bones, may offer a solution to this unfavorably selective SPION delivery. HAP nanoparticles are commended not only for their exceptional biocompatibility but also for the convenience of their use as an intracellular delivery agent. Here we demonstrate that dispersing SPIONs in HAP using a wet synthesis method could increase the uptake in cancer cells and minimize the risk to healthy cells. Specifically, HAP/SPION nanocomposites retain the superparamagnetic nature of SPIONs, increase the uptake ratio between U87 human brain cancer cells and human MSCs versus their SPION counterparts, reduce migration in a primary brain cancer spheroid model compared to the control, reduce brain cancer cell viability compared to the treatment with SPIONs alone, and retain the viability of healthy human MSCs. A functional synergy between the two components of the nanocomposites was established; as a result, the cancer versus healthy cell (U87/MSC) selectivity in terms of both the uptake and the toxicity was higher for the composite than for SPIONs or HAP alone, allowing it to be damaging to cancer cells and harmless to the healthy ones. The analysis of actin cytoskeleton order at the microscale revealed that healthy MSCs and primary cancer cells after the uptake of SPIONs display reduced and increased anisotropy in their cytoskeletal arrangement, respectively. In contrast, the uptake of SPION/HAP nanocomposites increased the cytoskeletal anisotropy of both the healthy MSCs and the primary cancer cells. In spite of the moderate specific magnetization of HAP/SPION nanohybrids, reaching 15 emu/g for the 28.6 wt % SPION-containing composite, the cancer cell treatment in an alternating magnetic field resulted in an intense hyperthermia effect that increased the temperature by ca. 1 °C per minute of exposure and reduced the cell population treated for 30 min by more than 50%, while leaving the control populations unharmed. These findings on nanocomposites of HAP and SPIONs may open a new avenue for cancer therapies that utilize MH.

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“…Ah igh concentrationw as observedi nl iver and spleen after uptake by macrophages. [124] The intravenous injection of calcium phosphate nanoparticles leads to ap ronouncedd istribution in the body,w ith liver, lung and spleen the most prominentt arget organs. Their longterm fate is unknown, but based on the known cell-biological effects, it can be safely assumed that they will be taken up by cells (including macrophages) and dissolved to the constituting harmlessi ons, that is, Ca 2 + and PO 4 3À ,u nlike other insoluble inorganic and polymeric nanoparticles.…”

Section: Biomedical Applications Of Calcium Phosphate Nanoparticlesmentioning

confidence: 99%

Biological and Medical Applications of Calcium Phosphate Nanoparticles

Sokolova

Epple

2021

Chemistry A European J

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Calcium phosphate nanoparticles have ah igh biocompatibility and biodegradability due to their chemical similarity to human hard tissue, for example, bone and teeth. They can be used as efficient carriers for different kinds of biomolecules such as nucleic acids, proteins, peptides, antibodies, or drugs,w hich alone are not able to enter cells where their biological effect is required. They can be loaded with cargom olecules by incorporating them, unlike solid nanoparticles, and also by surface functionalization.This offersp rotection,f or example, against nucleases,a nd the possibility forc ell targeting. If such nanoparticles are functionalized with fluorescing dyes, they can be appliedf or imagingi nv itro and in vivo. Synthesis, functionalization and cell uptake mechanismso fc alcium phosphate nanoparticles are discussed together with applications in transfection, gene silencing, imaging, immunization, and bone substitution. Biodistribution data of calcium phosphate nanoparticles in vivo are reviewed.

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Hydroxyapatite nanoparticles

Cited by 20 publications

References 39 publications

Development, Characterization and In Vitro Biological Properties of Scaffolds Fabricated From Calcium Phosphate Nanoparticles

Development, Characterization and In Vitro Biological Properties of Scaffolds Fabricated From Calcium Phosphate Nanoparticles

Hydroxyapatite as a Vehicle for the Selective Effect of Superparamagnetic Iron Oxide Nanoparticles against Human Glioblastoma Cells

Biological and Medical Applications of Calcium Phosphate Nanoparticles

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