Hydroxycinnamic acids and UV-B depletion: Profiling and biosynthetic gene expression in flesh and peel of wild-type and hp-1

Calvenzani, V.; Castagna, Antonella; Ranieri, Annamaria; Tonelli, Chiara; Petroni, Katia

doi:10.1016/j.jplph.2015.04.008

Cited by 8 publications

(3 citation statements)

References 43 publications

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“…These results may suggest that preferential accumulation of CA and FA in the peel is a part of acclimation response to direct exposure to solar radiation, while in the flesh other factors may influence the composition of HCAs. Similarly to our results for 2013 and 2014, Calvenzani et al (2015) reported that total concentration of HCAs in fully ripe tomato fruits was higher under ambient UV-B than in UV-B shielded fruits.…”

Section: Phenolic Compounds In Tomato Fruitssupporting

confidence: 91%

Contents of phenolics and carotenoids in tomato grown underpolytunnels with different UV-transmission rates

Živanović¹,

VIDOVIĆ²,

KOMIĆ³

et al. 2017

Turk J Agric For

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Tomato (Solanum lycopersicum L.) is among the economically most important vegetables in Europe, valued for its bioactive properties due to significant contents of vitamins, carotenoids, and phenolic compounds. In this study, the tomato cultivar Big Beef F1 was grown in the open field (OF) and under polytunnels in central Serbia during 3 years. Polytunnels were covered with two foils (both with 57% reduced photosynthetic active radiation, PAR) differing in UV-A and UV-B transmittance. The aim of our work was to determine the influence of light conditions on accumulation of phytonutrients (carotenoids and phenolics) in the peel and flesh of ripe tomato fruits. The amount of effective antioxidants, caffeic acid, and quercetin (phenolics with ortho-dihydroxy substitution) in the peel was the highest in tomato fruits grown in the OF (maximal PAR and UV-A and UV-B radiation). Moreover, the content of leaf epidermal flavonoids was the highest in the OF. The content of lycopene and β-carotene in the flesh of tomato fruit was higher under the polytunnel with higher UV-transmittance. Our results showed that selection of the right light conditions (quality and intensity) for tomato production has a significant effect on the accumulation of beneficial phenolics and carotenoids.

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Section: Phenolic Compounds In Tomato Fruitssupporting

confidence: 91%

Contents of phenolics and carotenoids in tomato grown underpolytunnels with different UV-transmission rates

Živanović¹,

VIDOVIĆ²,

KOMIĆ³

et al. 2017

Turk J Agric For

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“…In addition, UV Resistance Locus8 (UVR8) initiates the stress signaling response by receiving UV-B light [ 2 , 7 , 9 , 10 ]. Among UV-B stress responses, an important change in terms of secondary metabolites is the accumulation of phenolic compounds, such as flavonoids, anthocyanins, and hydroxycinnamic acids (HCAs), that function as sunscreens in vacuoles of epidermal cells to protect important subcellular components from UV-B light [ 11 , 12 , 13 ]. However, plant responses to UV-B light depend on its energy level, duration, and peak wavelength and on the plant species, varieties, and growth stages [ 14 , 15 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Physiologic and Metabolic Changes in Crepidiastrum denticulatum According to Different Energy Levels of UV-B Radiation

Park

Lee

et al. 2020

IJMS

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Ultraviolet B (UV-B) light, as a physical elicitor, can promote the secondary metabolites biosynthesis in plants. We investigated effects of different energy levels of UV-B radiation on growth and bioactive compounds of Crepidiastrum denticulatum. Three-week-old seedlings were grown in a plant factory for 5 weeks. Plants were subjected to different levels of UV-B (0, 0.1, 0.25, 0.5, 1.0, and 1.25 W m−2), 6 h a day for 6 days. All UV-B treatments had no negative effect on the shoot dry weight; however, relatively high energy treatments (1.0 and 1.25 W m−2) inhibited the shoot fresh weight. UV-B light of 0.1, 0.25, and 0.5 W m−2 did not affect total chlorophyll and H2O2 contents; however, they increased total carotenoid content. On 4 days, 0.25 W m−2 treatment increased antioxidant capacity, total hydroxycinnamic acids (HCAs) content, and several sesquiterpenes. Treatments with 1.0 and 1.25 W m−2 increased total carotenoid, total HCAs, and H2O2 contents, and destroyed chlorophyll pigments, reducing maximum quantum yield of photosystem II and causing visible damage to leaves. Partial least squares discrimination analysis (PLS-DA) showed that secondary metabolites were distinguishably changed according to energy levels of UV-B. The potential of 0.25 W m−2 UV-B for the efficient production of bioactive compounds without growth inhibition in C. denticulatum was identified.

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“…Phenolic acids are responsible for the astringent taste of tomato fruits and consist mainly of gallic, chlorogenic, and ferulic acids ( Moco et al, 2007 ). Hydroxycinnamates, due to their antioxidant capacity, have important beneficial health effects: they can limit LDL (low-density lipid) oxidation, prevent carcinogenesis and are potential therapeutic agents for neurodegenerative diseases, such as Alzheimer and Parkinson and for the prevention of cardiovascular disease and diabetes ( Niggeweg et al, 2004 ; Calvenzani et al, 2015 ; Tohge et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Metabolic and Molecular Changes of the Phenylpropanoid Pathway in Tomato (Solanum lycopersicum) Lines Carrying Different Solanum pennellii Wild Chromosomal Regions

Rigano

Raiola

Docimo³

et al. 2016

Front. Plant Sci.

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Solanum lycopersicum represents an important dietary source of bioactive compounds including the antioxidants flavonoids and phenolic acids. We previously identified two genotypes (IL7-3 and IL12-4) carrying loci from the wild species Solanum pennellii, which increased antioxidants in the fruit. Successively, these lines were crossed and two genotypes carrying both introgressions at the homozygous condition (DHO88 and DHO88-SL) were selected. The amount of total antioxidant compounds was increased in DHOs compared to both ILs and the control genotype M82. In order to understand the genetic mechanisms underlying the positive interaction between the two wild regions pyramided in DHO genotypes, detailed analyses of the metabolites accumulated in the fruit were carried out by colorimetric methods and LC/MS/MS. These analyses evidenced a lower content of flavonoids in DHOs and in ILs, compared to M82. By contrast, in the DHOs the relative content of phenolic acids increased, particularly the fraction of hexoses, thus evidencing a redirection of the phenylpropanoid flux toward the biosynthesis of phenolic acid glycosides in these genotypes. In addition, the line DHO88 exhibited a lower content of free phenolic acids compared to M82. Interestingly, the two DHOs analyzed differ in the size of the wild region on chromosome 12. Genes mapping in the introgression regions were further investigated. Several genes of the phenylpropanoid biosynthetic pathway were identified, such as one 4-coumarate:CoA ligase and two UDP-glycosyltransferases in the region 12-4 and one chalcone isomerase and one UDP-glycosyltransferase in the region 7-3. Transcriptomic analyses demonstrated a different expression of the detected genes in the ILs and in the DHOs compared to M82. These analyses, combined with biochemical analyses, suggested a central role of the 4-coumarate:CoA ligase in redirecting the phenylpropanoid pathways toward the biosynthesis of phenolic acids in the pyramided lines. Moreover, analyses here carried out suggest the presence in the introgression regions of novel regulatory proteins, such as one Myb4 detected on chromosome 7 and one bHLH detected in chromosome 12. Overall our data indicate that structural and regulatory genes identified in this study might have a key role for the manipulation of the phenylpropanoid metabolic pathway in tomato fruit.

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Hydroxycinnamic acids and UV-B depletion: Profiling and biosynthetic gene expression in flesh and peel of wild-type and hp-1

Cited by 8 publications

References 43 publications

Contents of phenolics and carotenoids in tomato grown underpolytunnels with different UV-transmission rates

Contents of phenolics and carotenoids in tomato grown underpolytunnels with different UV-transmission rates

Physiologic and Metabolic Changes in Crepidiastrum denticulatum According to Different Energy Levels of UV-B Radiation

Metabolic and Molecular Changes of the Phenylpropanoid Pathway in Tomato (Solanum lycopersicum) Lines Carrying Different Solanum pennellii Wild Chromosomal Regions

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