Hydroxycitric acid inhibits kidney stone through crystallization regulation and reduction of hyperoxalate-induced lipotoxicity

Zhang, Yi-Han; Xian, Jun-Yi; Li, Shujue; Li, Chun-Yao; Yu, Bang-Xian; Liang, Qing; Huang, Ling‐Hong; Zeng, Guohua; Sun, Xin-Yuan

doi:10.1016/j.jff.2023.105561

Cited by 3 publications

(2 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Immunohistochemical staining of kidney sections was conducted according to our previous study . The kidney sections were incubated overnight at 4 °C with antibodies against anti-KIM-1 (1:100, ab47635, Abcam), anti-NRF2 (1:200, 16396–1-AP, Proteintech), anti-Keap-1 (1:200, 10503–2-AP, Proteintech), anti-CAT (1:250, 21260–1-AP, Proteintech), anti-SOD (1:300, 10269–1-AP, Proteintech), anti-CD44 (1:500, 15675–1-AP, Proteintech), anti-OPN (1:250, 22952–1-AP, Proteintech), anti-IL-6 (1:200, A0286, Abclonal), and anti-MCP-1 (1:300, ab7202, Abcam).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Mechanism of Porphyra Yezoensis Polysaccharides in Inhibiting Hyperoxalate-Induced Renal Injury and Crystal Deposition

Deng,

Li,

Huang

et al. 2024

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

Oxidative damage to the kidneys is a primary factor in the occurrence of kidney stones. This study explores the inhibitory effect of Porphyra yezoensis polysaccharides (PYP) on oxalate-induced renal injury by detecting levels of oxidative damage, expression of adhesion molecules, and damage to intracellular organelles and revealed the molecular mechanism by molecular biology methods. Additionally, we validated the role of PYP in vivo using a crystallization model of hyperoxalate-induced rats. PYP effectively scavenged the overproduction of reactive oxygen species (ROS) in HK-2 cells, inhibited the adhesion of calcium oxalate (CaOx) crystals on the cell surface, unblocked the cell cycle, restored the depolarization of the mitochondrial membrane potential, and inhibited cell death. PYP upregulated the expression of antioxidant proteins, including Nrf2, HO-1, SOD, and CAT, while decreasing the expression of Keap-1, thereby activating the Keap1/Nrf2 signaling pathway. PYP inhibited CaOx deposition in renal tubules in the rat crystallization model, significantly reduced high oxalate-induced renal injury, decreased the levels of the cell surface adhesion proteins, improved renal function in rats, and ultimately inhibited the formation of kidney stones. Therefore, PYP, which has crystallization inhibition and antioxidant properties, may be a therapeutic option for the treatment of kidney stones.

show abstract

Section: Methodsmentioning

confidence: 99%

“…Immunohistochemical staining of kidney sections was conducted according to our previous study. 21 The kidney sections were incubated overnight at 4 °C with antibodies against anti-KIM-1 (1:100, ab47635, Abcam), anti-NRF2…”

Section: Observation Of Caox Crystal Depositionmentioning

confidence: 99%