Hydroxyethyl starch 130/0.4 or hypertonic saline solution to decrease inflammatory response in hemorrhagic shock?

Esnault, Pierre; Prunet, Bertrand; Cotté, Jean; Cungi, Pierre-Julien; Alonso, Audrey; Montcriol, Ambroise

doi:10.1186/cc13043

Cited by 2 publications

(2 citation statements)

References 6 publications

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“…Resuscitation with HES also exhibits an anti-inflammatory effect in the intestinal injury after hemorrhagic shock, by dropping TNF-α, IL-6 expressions, and NF-κB activation in ileum. In patients undergoing coronary surgery, resuscitation with HES has a higher level of TNF-α compared to a balanced electrolyte crystalloid solution 2 hours after surgery [8,36,37,45]. All the evidences above reveal that the resuscitation fluids could either directly or indirectly alter the expression of cytokines and activation of signaling pathways, and these changes would move outward to affect the MDSC and other immune system.…”

Section: Discussionmentioning

confidence: 99%

Effects of Hypertonic Saline and Hydroxyethyl Starch on Myeloid‐Derived Suppressor Cells in Hemorrhagic Shock Mice under Secondary Bacterial Attack

Jiang

Ling

Lü

2020

BioMed Research International

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Objectives. The primary target is to reveal whether the resuscitation with hypertonic saline (HTS) or hydroxyethyl starch (HES) would have different effects on the myeloid-derived suppressor cell (MDSC) count and monocytic MDSC (M-MDSC)/granulocytic/neutrophilic MDSC (G-MDSC) rate in the peripheral blood, spleen, and bone marrow nucleated cells (BMNC) in a controlled hemorrhagic shock mouse model under secondary Escherichia coli bacterial infection attack, comparing to resuscitation with normal saline (NS) in 72 hours. Method. After hemorrhagic shock with bacteremia, which is induced by Escherichia coli 35218 injection, the mice were distributed into control, NS, HTS, and HES groups. The peripheral blood nucleated cells (PBNC), spleen single-cell suspension, and bone marrow nucleated cells were collected. The flow cytometry was used to detect the MDSC, M-MDSC, and G-MDSC. Result. In PBNC, after resuscitation with NS, the MDSC was continuously higher, while the rate of M-MDSC/G-MDSC were continuously lower (P<0.05). In HTS, the MDSC varied, higher at 24 and 72 hours (P<0.05), the M-MDSC/G-MDSC were continuously lower (P<0.05). In the spleen, resuscitation with HTS, the M-MDSC/G-MDSC were continuously lower (P<0.05). In BMNC, after resuscitation with HES, the M-MDSC/G-MDSC were lower at 24 and 72 hours (P<0.05). Conclusion. In mouse hemorrhagic shock model with bacterial infection, the resuscitation with NS, HTS, or HES induced difference changes in MDSC and M-MDSC/G-MDSC, which were time-dependent and organ-specific. Resuscitation with crystalloid, like NS or HTS, showed longer effects on the MDSC and M-MDSC/G-MDSC in peripheral blood; while HTS has a longer effect on M-MDSC/G-MDSC in the spleen, HES has a stronger impact on the differentiation regulation of MDSC to G-MDSC in the bone marrow.

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Section: Discussionmentioning

confidence: 99%

Effects of Hypertonic Saline and Hydroxyethyl Starch on Myeloid‐Derived Suppressor Cells in Hemorrhagic Shock Mice under Secondary Bacterial Attack

Jiang

Ling

Lü

2020

BioMed Research International

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“…Studies based on different animal models suggest that HTS and HES could either directly or indirectly alter the expression of cytokines and activation of signaling pathways, including those above (Coimbra et al, 1996;Loomis et al, 2001;Ke et al, 2006;Wang et al, 2009;Chen et al, 2013;Esnault et al, 2013;Liu et al, 2013;Choi et al, 2014;Dong et al, 2014;Huang et al, 2014;Igarashi et al, 2014;Wright et al, 2014;Öztürk et al, 2015;Chang et al, 2016). Thus, the MDSC-related cytokines and signaling pathways in hemorrhagic shock resuscitation with different kinds of fluids need further research.…”

Section: Fig 3 Variations Of Mdscs and M-mdsc/g-mdsc Ratio In Mice Bmentioning

confidence: 99%

Distribution and differentiation of myeloid-derived suppressor cells after fluid resuscitation in mice with hemorrhagic shock

Jiang

Fang

Ling

et al. 2017

J. Zhejiang Univ. Sci. B

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Abstract:Objective: To investigate the distribution and differentiation of myeloid-derived suppressor cells (MDSCs) in hemorrhagic shock mice, which are resuscitated with normal saline (NS), hypertonic saline (HTS), and hydroxyethyl starch (HES). Methods: BALB/c mice were randomly divided into control, NS, HTS, and HES resuscitation groups. Three subgroups (n=8) in each resuscitation group were marked as 2, 24, and 72 h. Flow cytometry was used to detect the MDSCs, monocytic MDSCs (M-MDSCs), and granulocytic/neutrophilic MDSCs (G-MDSCs) in peripheral blood nucleated cells (PBNCs), spleen single-cell suspension, and bone marrow nucleated cells (BMNCs). Results: The MDSCs in BMNCs among three resuscitation groups were lower 2 h after shock, in PBNCs of the HTS group were higher, and in spleen of the NS group were lower (all P<0.05 vs. control). The M-MDSC/G-MDSC ratios in PBNCs of the HTS and HES groups were lower (both P<0.05 vs. control). At 24 h, the MDSCs in PBNCs of the NS and HTS groups were higher, while the spleen MDSCs in the HTS group were higher (all P<0.05 vs. control). The M-MDSC/ G-MDSC ratios were all less in PBNCs, spleen, and BMNCs of the NS and HTS groups, and were lower in BMNCs of the HES group (all P<0.05 vs. control). At 72 h, the elevated MDSCs in PBNCs were presented in the HTS and HES groups, and in spleen the augment turned up in three resuscitation groups (all P<0.05 vs. control). The inclined ratios to M-MDSC were exhibited in spleen of the NS and HTS groups, and in PBNCs of the NS group; the inclination to G-MDSC in BMNCs was shown in the HES group (all P<0.05 vs. control). Conclusions: HTS induces the earlier elevation of MDSCs in peripheral blood and spleen, and influences its distribution and differentiation, while HES has a less effect on the distribution but a stronger impact on the differentiation of MDSCs, especially in bone marrow.

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Hydroxyethyl starch 130/0.4 or hypertonic saline solution to decrease inflammatory response in hemorrhagic shock?

Cited by 2 publications

References 6 publications

Effects of Hypertonic Saline and Hydroxyethyl Starch on Myeloid‐Derived Suppressor Cells in Hemorrhagic Shock Mice under Secondary Bacterial Attack

Effects of Hypertonic Saline and Hydroxyethyl Starch on Myeloid‐Derived Suppressor Cells in Hemorrhagic Shock Mice under Secondary Bacterial Attack

Distribution and differentiation of myeloid-derived suppressor cells after fluid resuscitation in mice with hemorrhagic shock

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