Hydroxyl radical concentration profile in photo-Fenton oxidation process: Generation and consumption of hydroxyl radicals during the discoloration of azo-dye Orange II

Maezono, T.; Tokumura, Masahiro; Sekine, Makoto; Kawase, Y.

doi:10.1016/j.chemosphere.2010.11.052

Cited by 198 publications

(85 citation statements)

References 27 publications

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“…However, the •OH is a very active species and its half-life time is as short as 10 −10 s. Although the increasing of the initial Fe concentration resulted in more •OH radical production even at higher initial Fe 2+ concentrations, excess •OH radicals would tend to react with other •OH radicals. The current signal recovers when Fe 2+ concentration goes up after 1 mM [45,46]. Under our incubation conditions, the optimum response was achieved at 1 mM FeSO 4 and 50 mM glucose (Fig.…”

Section: The Optimum Concentration Of Feso 4 and Glucosementioning

confidence: 76%

Electrochemical detection of in situ DNA damage induced by enzyme-catalyzed Fenton reaction. Part I: in phosphate buffer solution

et al. 2012

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We report on a biosensor for the electrochemical detection of the damage of DNA and of antioxidant protecting DNA. The biosensor was constructed by co-immobilization of DNA and glucose oxidase (GOx) on a glassy carbon electrode. Under aerobic conditions, GOx catalyzes the oxidation of glucose, and the hydrogen peroxide produced reacts with ferrous ions in a Fenton-type reaction to generate hydroxy radical. This was validated by UV-vis spectroscopy. The hydroxy radical can cause serious oxidative damage to DNA, and this can be detected by square wave voltammetry of the electroactive indicator Co(bpy) 3 3+. The effects of pH value, incubation time, and the concentration of glucose and ferrous ion were optimized. The effects of the antioxidants ascorbic acid and aloe emodin on DNA damage were also investigated within the concentration range from 0.05 to 200 μM. This work provides an in-vitro model system to mimic the processes in oxidative DNA damage by a simple electrochemical approach.

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Section: The Optimum Concentration Of Feso 4 and Glucosementioning

confidence: 76%

Electrochemical detection of in situ DNA damage induced by enzyme-catalyzed Fenton reaction. Part I: in phosphate buffer solution

et al. 2012

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“…After reaching of the maximum, excess Fe 2+ might cause scavenger effects. Although the increasing of the initial Fe 2+ concentration resulted in more •OH radical production, excess •OH would tend to react with other •OH [24,25]. In the clevage agent containing 1.5 mM FeSO 4 and varying concentration of glucose, I pt /I p0 decreased rapidly and then trended to a constant value at 75 mM (Fig.…”

Section: Effect Of Feso 4 and Glucose Concentration On Dna Damagementioning

confidence: 88%

Electrochemical detection of in situ DNA damage induced by enzyme-catalyzed Fenton reaction. Part II in hydrophobic room temperature ionic liquid

et al. 2012

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A hydrophobic room temperature ionic liquid, 1-butyl-3-methylimidazolium hexafluorophosphate ([BMIM] [PF 6 ]), was applied as nonaqueous solvent for the generation of hydroxy radical (•OH) through glucose oxidasecatalyzed Fenton reaction. The enzyme catalyzes the oxidation of glucose, and the produced H 2 O 2 further reacts with transition metal ions, generating hydroxyl radicals. They attacked DNA and led its damage. This was detected by square wave voltammetry (SWV) of the electroactive indicator Co(bpy) 3 3+ . It bound more strongly to intact DNA, and the SWV peak currents decreased at the potential of 0.064 V when DNA was damaged. The experimental results testified that the antioxidants, ascorbic acid, aloe-emodin and rutin, inhibited oxidative DNA damage by hydroxyl radicals. The method is promising for rapid, sensitive, and inexpensive detection of DNA damage.

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“…Thus, the concentration of hydrogen peroxide is one of the key factors to the synergism of the heterogeneous sono-Fenton process. There are several ways to determine the concentration of hydrogen peroxide including spectrophotometry (Papoutsakis et al 2015), iodometric titration (Segura et al 2012), and using glucose oxidase (Maezono et al 2011). However, all these methods involve sampling and chemical addition, potentially adding error to the results.…”

Section: Introductionmentioning

confidence: 99%

Real-time detection of hydrogen peroxide using microelectrodes in an ultrasonic enhanced heterogeneous Fenton process catalyzed by ferrocene

Lin

Qing

Gao

2015

Environ Sci Pollut Res

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Microelectrodes were used for real-time detection of hydrogen peroxide (H2O2) in a heterogeneous sono-Fenton system with ferrocene as the catalyst. The working mechanism of reactive blue 13 decolorization in a heterogeneous sono-Fenton system was investigated. Ultrasonic irradiation showed no effect on decolorization when used alone and did not enhance decolorization in the H2O2 system (43.0 % for H2O2 vs. 48.1 % for US+H2O2). However, a system with the presence of Fenton-like reagents achieved complete decolorization. Decolorization was greatly accelerated by the addition of ultrasonic irradiation. Thorough decolorization was achieved in 20 min in the heterogeneous sono-Fenton system, which was 30 min faster than in the heterogeneous Fenton system. Based on the data collected by microelectrodes, accelerated decomposition of H2O2 was also observed. Ultrasonic irradiation aided the ferrocene catalyst in liberating more •OH from Fenton reactions, leading to the faster decolorization.

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Hydroxyl radical concentration profile in photo-Fenton oxidation process: Generation and consumption of hydroxyl radicals during the discoloration of azo-dye Orange II

Cited by 198 publications

References 27 publications

Electrochemical detection of in situ DNA damage induced by enzyme-catalyzed Fenton reaction. Part I: in phosphate buffer solution

Electrochemical detection of in situ DNA damage induced by enzyme-catalyzed Fenton reaction. Part I: in phosphate buffer solution

Electrochemical detection of in situ DNA damage induced by enzyme-catalyzed Fenton reaction. Part II in hydrophobic room temperature ionic liquid

Real-time detection of hydrogen peroxide using microelectrodes in an ultrasonic enhanced heterogeneous Fenton process catalyzed by ferrocene

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