Hydroxyl radical scavengers enhance nitric oxide-evoked acetylcholine release from mouse cerebral cortical neurons

Ohkuma, Seitaro; Katsura, Masashi; Chen, Da-Zhi; Guo, Jin-Long; Kuriyama, Kinya

doi:10.1016/0169-328x(95)00207-9

Cited by 10 publications

(4 citation statements)

References 12 publications

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“…Effect of Radical Scavengers. Assuming that reactive chemical species, such as OH radicals and/or Cl radicals originating from HOCl, are involved in the disinfectant process, we examined the effect of pretreatment of the EW(+) before exposure to AFB1 with mannitol that has been regarded as OH radical specific scavenger (26) and thiourea that has been regarded as a universal radical scavenger (27,28). Mannitol was added to the EW(+) at ratios of 1 to 10 (molar ratio of mannitol to available chlorine as HOCl); similarly, thiourea was added to the EW(+) at ratios of 0.03 to 1 (molar ratio of thiourea to available chlorine as HOCl).…”

Section: Chemicals Andmentioning

confidence: 99%

Decontamination of Aflatoxin-Forming Fungus and Elimination of Aflatoxin Mutagenicity with Electrolyzed NaCl Anode Solution

Suzuki

Noro

Kawamura

et al. 2001

J. Agric. Food Chem.

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Electrolysis of a 0.1% (17.1 mM) solution of NaCl using separate anode and cathode compartments gives rise to solutions containing active chemical species. The strongly acidic "anode solution" (EW+) has high levels of dissolved oxygen and available chlorine in a form of hypochlorous acid (HOCl) with a strong potential for sterilization, which we have investigated here. Exposing Aspergillus parasiticus at an initial density of 10(3)spores in 10 microL to a 50-fold volume (500 microL) of EW+ containing ca. 390 micromol HOCl for 15 min at room temperature resulted in a complete inhibition of fungal growth, whereas the cathode solution (EW-) had negligible inhibitory effects. Moreover, the mutagenicity of aflatoxin B(1) (AFB(1)) for Salmonella typhimurium TA-98 and TA-100 strains was strongly reduced after AFB(1) exposure to the EW+ but not with the EW-. In high-performance liquid chromatography analysis, the peak corresponding to AFB(1) disappeared after treatment with the EW+, indicating decomposition of the aflatoxin. In contrast, the routinely used disinfectant sodium hypochlorite, NaOCl, of the same available chlorine content as that of EW+ but in a different chemical form, hypochlorite (OCl-) ion, did not decompose AFB(1) at pH 11. However, NaOCl did decompose AFB(1) at pH 3, which indicated that the principle chemical formula to participate in the decomposition of AFB(1) is not the OCl- ion but HOCl. Furthermore, because the decomposition of AFB(1) was suppressed by pretreating the EW+ with the OH radical scavenger thiourea, the chemical species responsible for the AFB(1)-decomposing property of the EW+ should be at least due to the OH radical originated from HOCl. The OH in EW+ was proved by electron spin resonance analysis.

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Section: Chemicals Andmentioning

confidence: 99%

Decontamination of Aflatoxin-Forming Fungus and Elimination of Aflatoxin Mutagenicity with Electrolyzed NaCl Anode Solution

Suzuki

Noro

Kawamura

et al. 2001

J. Agric. Food Chem.

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“…AV: Average; SD; Standard variation; PC: Percent change over the control; * P <:0.01 DISCUSSION: They 2 have cited that alcohol alters the Ach levels and Ach further evoke significant membrane depolarization in rat major pelvic ganglion. Ach evoking large long-term effects on NMDA receptors in rats has been reported by 4 . They 3 studied the effect of ethyl alcohol on the contractile activities of canine gastric corpus and fundus circular muscles and they reported that alcohol alteres the normal functioning of Ach 23 , showed ethanol as to modulate the receptor c-protein function in the rat cerebral cortex.…”

Section: Resultsmentioning

confidence: 94%

“…Effects on basal or stimulated Ach release have been described in mammalian brain 1,2,3,4,5,6 . Aplysia ganglious 7,8 and smooth muscle Likewise NO and Ach are interrelated with regard to their physiological functioning 20 21 .…”

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confidence: 99%

Untitled

2014

IJPSR

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Acetylcholine satisfies all criteria for a transmitter substance. Its most widely recognized action sites are the endings of vertebrate motor nerves, the endings of the automatic pre ganglionics and the parasympathetic post ganglionics. It appears also to be the transmitter of motor nerves in all advanced invertebrate animals except the arthropods where it seems to separate at the endings of sensory nerves. Acetylcholine is concerned with various visceral functions in both invertebrates and vertebrates. The impact of 20% ethanol (w/v) at selected doses of 5gm/kg wt over 5 and10 weeks

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“…, 1986) and deferoxamine is also known as an iron chelator. In a study of murine cerebral cortical neurons, mannitol increased NO‐induced acetylcholine release (Ohkuma et al. , 1995).…”

Section: Discussionmentioning

confidence: 99%

Investigation of enhancement effects of nicotine on cholinergic neurotransmission in isolated rabbit gastric fundus: role of antioxidants

Anuvarbekova

Fincan

Vural

et al. 2010

Auton Autocoid Pharmacol

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Nicotine, which is tobacco alkaloid, still induces interests for researchers because of smokers addiction to nicotine. Nicotine having influence on the neuronal acetylcholine receptors (nAChRs) increases release of most certain neurotransmitters from the nerve endings. Also, nicotine, affecting the mitochondrial respiratory chains, contributes to the formation of reactive oxygen species. In the present study, we investigated the effects of nicotine on smooth muscles of gastric fundus on the electrical field stimulation (EFS) that induces transition contraction via stimulation nAChRs. In addition, we aimed to investigate the interaction between release of acetylcholine, induced by nicotine, and the effects of reactive oxygen species. Therefore, the effects of allopurinol (10(-6)-10(-5) M), deferoxamine (10(-4) M) and mannitol (10(-4)-5 x 10(-3) M) were tested on the transient contraction induced by nicotine. In conclusion, mannitol (5 x 10(-3) M) significantly reduced contractile response to nicotine on EFS only in high concentration. Whereas in small concentrations mannitol (10(-4) M) statistically did not cause any results. Deferoxamine and allopurinol also did not have any significant response.

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Hydroxyl radical scavengers enhance nitric oxide-evoked acetylcholine release from mouse cerebral cortical neurons

Cited by 10 publications

References 12 publications

Decontamination of Aflatoxin-Forming Fungus and Elimination of Aflatoxin Mutagenicity with Electrolyzed NaCl Anode Solution

Decontamination of Aflatoxin-Forming Fungus and Elimination of Aflatoxin Mutagenicity with Electrolyzed NaCl Anode Solution

Untitled

Investigation of enhancement effects of nicotine on cholinergic neurotransmission in isolated rabbit gastric fundus: role of antioxidants

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