Hydroxylation of 16α-methyl-17α,21-dihydroxypregn-4-en-3-one

The biosynthesis of steroids and triterpenoids

“…858 (An 11 -hydroxylase from the fungus Curvularia lunata has also been shown to catalyse 11 -hydroxylation of a variety of steroids including 11-deoxycortisol and DOC.) 859,860 The putative steroid-and heme-binding regions of P-450 (11 ) have been identified by amino acid sequence alignment of these enzymes from various animal species. 833 18-Vinylprogesterone and 18-ethynylprogesterone have both been found to be potent mechanism-based inhibitors of aldosterone biosynthesis 861 and 18-vinylprogesterone preferentially inhibits the 18-hydroxylation step.…”