2013
DOI: 10.1007/s10517-013-1935-5
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Hydroxyproline Content and Fibrogenesis in Mouse Liver and Lungs during the Early Stages of BCG Granulomatosis

Abstract: In generalized BCG granulomatosis, fibrosis starts early (on day 3) and not only around the granulomas, but also in the organs. The severity of organ fibrosis is apparently determined by the concentration of granulomas, in particular their macrophages inducing proliferation of fibroblasts in organs and granulomas as well as activation of fibrogenesis. On day 30 after infection, the degree of fibrosis in the lungs was by 6 times higher than in the liver. The increase in hydroxyproline concentration in organs in… Show more

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Cited by 8 publications
(3 citation statements)
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“…Free splenic macrophages and macrophages that had emigrated from BCG granulomas did not contain BCG mycobacteria in their cytoplasm according to the fluorescent analysis based on using acridine orange for mycobacteria visualization (5). The phagocytic activity study of splenic macrophages and macrophages from BCG granulomas was performed one day after in vitro explantation of splenic macrophages and BCG granulomas (12,16,10). BCG vaccine mycobacteria were added to all investigated cultures in 50x10 6microbes/ml concentration, incubated at 37°C for 5 h and stained with acridine orange (10 ug/ml) for 15 min and with safranin (10 ug/rnl) for 5 min.…”
Section: Cytophisiological Studies Ofmacrophagesmentioning
confidence: 99%
See 1 more Smart Citation
“…Free splenic macrophages and macrophages that had emigrated from BCG granulomas did not contain BCG mycobacteria in their cytoplasm according to the fluorescent analysis based on using acridine orange for mycobacteria visualization (5). The phagocytic activity study of splenic macrophages and macrophages from BCG granulomas was performed one day after in vitro explantation of splenic macrophages and BCG granulomas (12,16,10). BCG vaccine mycobacteria were added to all investigated cultures in 50x10 6microbes/ml concentration, incubated at 37°C for 5 h and stained with acridine orange (10 ug/ml) for 15 min and with safranin (10 ug/rnl) for 5 min.…”
Section: Cytophisiological Studies Ofmacrophagesmentioning
confidence: 99%
“…Granulomagenic chemoattractants include granulocyte-macrophage colony-stimulating factor (GM-CSF), tumor necrosis factor alpha (TNF-a) and interleukin (IL)-l (2,(7)(8)(9). The large-scale activated macrophage (whose destiny is unknown) exiting from granulomas (2,3,10) was observed during the natural elimination of mycobacteria from macrophages or the elimination induced by successful drug therapy. It involves the decrease of granuloma size and number as well as the proportion of phagocytic cells in granulomas due to their destruction and emigrating phagocytes (2,3).…”
mentioning
confidence: 99%
“…Traditionally, lung fibrosis has been considered a result of predominantly chronic inflammation [12][13][14]. However, as shown in an experiment on a mice model of Mycobacterium bovis bacillus Calmette-Guérin (BCG)-induced generalized inflammation, fibrosis develops early, more precisely, 3 days after infecting, even before the development of destructive processes in the organ and chronic inflammation [15].…”
Section: Introductionmentioning
confidence: 99%