Hydroxysafflor yellow A suppresses angiogenesis of hepatocellular carcinoma through inhibition of p38 MAPK phosphorylation

Zhang, Jingyu; Li, Jingmin; Song, Haoran; Xiong, Yanlian; Liu, Desheng; Bai, Xianyong

doi:10.1016/j.biopha.2018.09.086

Cited by 36 publications

(23 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This variation may be associated with the applied stimuli and duration as well as the specific characteristics and types of cells. In vitro and in vivo research demonstrated that hydroxysafflor yellow A induces the apoptosis of HepG2 cells by substantially inhibiting the phosphorylation of the p38MAPK pathway [31]. Other evidence reveals that NK007 induces G1/S arrest through the activation of phosphorylated p38MAPK expression and degradation of HK2 expression associated with acidification and oxygen consumption rates [32].…”

Section: Discussionmentioning

confidence: 99%

Licochalcone A-Induced Apoptosis Through the Activation of p38MAPK Pathway Mediated Mitochondrial Pathways of Apoptosis in Human Osteosarcoma Cells In Vitro and In Vivo

Lin

Yang

Chiou

et al. 2019

Cells

View full text Add to dashboard Cite

Background: Licochalcone A (LicA) is isolated from the roots of Glycyrrhiza glabra and possesses antitumor and anti-invasive activities against several tumor cells. However, the antitumor effects of LicA on human osteosarcoma cells have yet to be demonstrated either in vitro or in vivo. Methods: Cell viability was measured by MTT assay. Apoptosis and mitochondrial dysfunction were detected with Annexin V/PI staining and JC-1 staining by flow cytometry. The expressions of caspase- or mitochondrial-related proteins were demonstrated by western blotting. Antitumor effect of LicA on 143B xenograft mice in vivo. Results: LicA could inhibit cell proliferation and induce apoptosis in human osteosarcoma cells, as evidenced by a decrease in cell viability, loss of mitochondrial membrane potentials, and activation of caspases. LicA treatment substantially reduced the expression of Bcl-2 and Mcl-1 and increased the expression of cleaved-caspase-3, cleaved-caspase-9, cleaved-PARP, and Bax in HOS and U2OS cells. Moreover, mitochondrial membrane potential and apoptosis suppression mediated by Z-VAD or tauroursodeoxycholic acid significantly reduced LicA-induced mitochondria-dependent apoptosis. The study also determined that LicA treatment induced p38MAPK phosphorylation, but siRNA-p38 or BIRB796 substantially reversed cell viability through the inhibition of mitochondria-dependent apoptosis pathways. Finally, an in vivo study revealed that LicA significantly inhibited 143B xenograft tumor growth. Conclusions: These findings demonstrate that LicA has antitumor activities against human osteosarcoma cells through p38MAPK regulation of mitochondria-mediated intrinsic apoptotic pathways in vitro and in vivo.

show abstract

Section: Discussionmentioning

confidence: 99%

Licochalcone A-Induced Apoptosis Through the Activation of p38MAPK Pathway Mediated Mitochondrial Pathways of Apoptosis in Human Osteosarcoma Cells In Vitro and In Vivo

Lin

Yang

Chiou

et al. 2019

Cells

View full text Add to dashboard Cite

show abstract

“…The anti-cancer effects of HSYA were investigated in mice, where HSYA effectively blocked proliferation and migration and induced apoptosis, providing evidence as of its being an anti-cancer agent for human hepatocellular carcinoma (HCC) [76]. HSYA injected at 1.13 mg/kg in mice reduced the proportion of Tregs within the spleen and enhanced the immunity of mice, exerting an anti-cancer effect [77].…”

Section: Anti-cancer Effectsmentioning

confidence: 99%

A Metabolic Perspective and Opportunities in Pharmacologically Important Safflower

et al. 2020

View full text Add to dashboard Cite

Safflower (Carthamus tinctorius L.) has long been grown as a crop due to its commercial utility as oil, animal feed, and pharmacologically significant secondary metabolites. The integration of omics approaches, including genomics, transcriptomics, metabolomics, and proteomics datasets, has provided more comprehensive knowledge of the chemical composition of crop plants for multiple applications. Knowledge of a metabolome of plant is crucial to optimize the evolution of crop traits, improve crop yields and quality, and ensure nutritional and health factors that provide the opportunity to produce functional food or feedstuffs. Safflower contains numerous chemical components that possess many pharmacological activities including central nervous, cardiac, vascular, anticoagulant, reproductive, gastrointestinal, antioxidant, hypolipidemic, and metabolic activities, providing many other human health benefits. In addition to classical metabolite studies, this review focuses on several metabolite-based working techniques and updates to provide a summary of the current medical applications of safflower.

show abstract

“…The evidence revealed that nK007 induces G1/S cell cycle arrest by promoting p38-MAPK phosphorylation and suppressing the expression of hexokinase2, which is associated with acidification and oxygen consumption in ovarian cells (29). In vivo and in vitro studies demonstrated that serotonin yellow A induces apoptosis of HepG2 cells by significantly inhibiting the phosphorylation of the p38-MAPK pathway in hepatocellular carcinoma cells (30). Other findings have demonstrated that calycosin can inhibit growth and induce apoptosis in breast cancer cells via estrogen receptor β-dependent regulation of the insulin-like growth factor 1 receptor, p38 MaPK and Pi3K/akt pathways in breast cancer cells (15).…”

Section: Discussionmentioning

confidence: 99%

Calycosin induces apoptosis via p38‑MAPK pathway‑mediated activation of the mitochondrial apoptotic pathway in human osteosarcoma 143B cells

et al. 2020

View full text Add to dashboard Cite

Previous studies have demonstrated that calycosin is a natural phytoestrogen with a similar structure to estrogen, which can inhibit cell proliferation and induce apoptosis in a variety of tumors. calycosin exerts potential pharmacological effects on osteosarcoma cells by inducing apoptosis. The aim of the present study was to elucidate the specific molecular mechanism of calycosin-induced apoptosis in osteosarcoma cells. cell proliferation was determined by an MTT assay. annexin V/Pi and Jc-1 staining were used to detect apoptosis and mitochondrial dysfunction, respectively, by flow cytometry. Western blot analysis was used to detect the expression of caspases or mitochondrial proteins. The results revealed that calycosin reduced the cell viability of human osteosarcoma 143B cells, induced apoptosis and increased the loss of mitochondrial membrane potential (MMP). in addition, calycosin increased the expression of the proapoptotic antiapoptotic proteins cleaved caspase-3, cleaved caspase-9, cleaved poly(adP-ribose) polymerase and Bcl-2-associated X protein (Bax), and decreased the expression of the antiapoptotic proapoptotic protein B-cell lymphoma-2 (Bcl-2), thus altering the Bax/Bcl-2 ratio. in addition, the expression levels of cytochrome c were markedly decreased in the mitochondria and increased in the cytoplasm following calycosin treatment. Furthermore, calycosin treatment induced p38-mitogen-activated protein kinase (MaPK) phosphorylation, whereas the p38-MaPK inhibitor BirB 796 markedly reversed cell viability, apoptosis and loss of MMP in 143B cells. These results suggested that calycosin inhibited osteosarcoma 143B cell growth via p38-MaPK regulation of mitochondrial-dependent intrinsic apoptotic pathways.

show abstract

Hydroxysafflor yellow A suppresses angiogenesis of hepatocellular carcinoma through inhibition of p38 MAPK phosphorylation

Cited by 36 publications

References 32 publications

Licochalcone A-Induced Apoptosis Through the Activation of p38MAPK Pathway Mediated Mitochondrial Pathways of Apoptosis in Human Osteosarcoma Cells In Vitro and In Vivo

Licochalcone A-Induced Apoptosis Through the Activation of p38MAPK Pathway Mediated Mitochondrial Pathways of Apoptosis in Human Osteosarcoma Cells In Vitro and In Vivo

A Metabolic Perspective and Opportunities in Pharmacologically Important Safflower

Calycosin induces apoptosis via p38‑MAPK pathway‑mediated activation of the mitochondrial apoptotic pathway in human osteosarcoma 143B cells

Contact Info

Product

Resources

About