Hydroxytyrosol, a natural antioxidant from olive oil, prevents protein damage induced by long-wave ultraviolet radiation in melanoma cells

D’Angelo, Stefania; Ingrosso, Diego; Migliardi, Valentina; Sorrentino, Alvara; Donnarumma, Giovanna; Baroni, Adone; Masella, L; Tufano, Maria Antonietta; Zappia, Marcello; Galletti, Patrizia

doi:10.1016/j.freeradbiomed.2004.12.015

Cited by 153 publications

(85 citation statements)

References 70 publications

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“…The increase on the level of Bad, Bax, active caspase-3 proteins, which we obtained from MDAH-2774 cell line is in concordance with the results of HT regarding the increase in the expressions of Bad, Bak and Bax, the pro-apoptotic proteins, on the human colon cancer HT-29 cell line 8 and the increase in the caspase-3 activity at the doses over 600-800 µM in the human melanoma cells (M14) 20 . In our study, it was determined that HT caused a decrease in the amounts of Bcl-2 and Pro-caspase-3, which are among the anti-apoptotic molecules, in OVCAR-3 cell lines and in the amounts of CIAP-1, CIAP-2, XIAP, Livin and Survivin, which belong to AIP family, while giving rise to an increase in the amount of pro-apoptotic cytochrome-c.…”

Section: Kip1supporting

confidence: 89%

Cytotoxic and Apoptotic Effects of Hydroxytyrosol on Ovarian Cancer Cell Lines: Relationship of Cytotoxic Effect with ERK 1/2 Pathway

Oktay¹,

Tuğrul²

2016

Cumhuriyet Med J

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SUMMARYObjective: It has been shown that hydroxytryrosol (HT) has antitumoral, anti-proliferative and apoptotic effects on various cancer cell line types. In this study, we investigated cytotoxic and apoptotic effects of HTon human ovarian cancer cell lines OVCAR-3 and MDAH-2774 and relationship of its cytotoxic effects with phosphatidylinositol 3-kinase/Akt (PI3K/Akt) and extracellular signal-regulated kinase 1/2 (ERK 1/2) signaling pathways. Method: XTT cell proliferation kit, Cell Death Detection Elisa Plus Kit and Human Apoptosis Array test were used to determine the cytotoxic and apoptotic effects of HT in OVCAR-3 and MDAH-2774 cell lines at 24, 48, 72 and 96 hours (h). Effect of HT on PI3K/Akt and ERK 1/2 signaling pathways were investigated by using specific inhibitors of these pathways. Results: IC50 values of HT were found to be 102.3 μM in MDAH-2774 cells at 72 h and 51.5 μM in OVCAR-3 cells at 96 h. Highest apoptotic effect was seen at 100 μM concentration in OVCAR-3 cells at 96 h and at 50 μM at 72 h in MDAH-2774. HT increased the levels of pro-apoptotic molecules including Bad, Bax, active caspase-3, Htra2/Omi and of proapoptotic death receptors TRAIL R1/DR4, TRAIL R2/DR5, FAS/TNFRSF6 and decreased Survivin levels in MDAH-2774 cells. In OVCAR-3 cells, HT decreased Bcl-2, pro-caspase 3 and CIAP-1, CIAP-2, XIAP, Livin, Survivin levels and increased cytochrome-c level. The cell vitality was increased in both of the cell lines as the result of the treatment with HT and FR180204. Conclusions: These results show that HT has potential cytotoxic and apoptotic effect on OVCAR-3 and MDAH-2774 cells. In both cell lines, the cytotoxic effect of HT could be ocur inhibiting through ERK 1/2 signaling pathway.

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Section: Kip1supporting

confidence: 89%

Cytotoxic and Apoptotic Effects of Hydroxytyrosol on Ovarian Cancer Cell Lines: Relationship of Cytotoxic Effect with ERK 1/2 Pathway

Oktay¹,

Tuğrul²

2016

Cumhuriyet Med J

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“…Several studies report the antioxidant properties of HT in biological systems (Cornwell and Ma 2008;D'Angelo et al 2005;Hamden et al 2009;Jemai et al 2009;Manna et al 1999;O'Dowd et al 2004;Visioli et al 1998). However, the mechanisms leading to the antioxidant properties of HT are not completely understood.…”

Section: Discussionmentioning

confidence: 99%

MnSOD activity regulates hydroxytyrosol-induced extension of chronological lifespan

Sarsour

Kumar

Kalen

et al. 2011

AGE

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Chronological lifespan (CLS) is defined as the duration of quiescence in which normal cells retain the capacity to reenter the proliferative cycle. This study investigates whether hydroxytyrosol (HT), a naturally occurring polyphenol found in olives, extends CLS in normal human fibroblasts (NHFs). Quiescent NHFs cultured for a long duration (30-60 days) lose their capacity to repopulate. Approximately 60% of these cells exit the cell cycle permanently; a significant increase in the doubling time of the cell population was observed. CLS was extended in quiescent NHFs that were cultured in the presence of HT for 30-60 days. HT-induced extension of CLS was associated with an approximately 3-fold increase in manganese superoxide dismutase (MnSOD) activity while there was no change in copper-zinc superoxide dismutase, catalase, or glutathione peroxidase protein levels. Quiescent NHFs overexpressing a dominant-negative mutant form of MnSOD failed to extend CLS. HT suppressed ageassociated increase in mitochondrial ROS levels. Results from spectroscopy assays indicate that HT in the presence of peroxidases can undergo catechol-semiquinone-quinone redox cycling generating superoxide, which in a cellular context can activate the antioxidant system, e.g., MnSOD expression. These results demonstrate that HT extends CLS by increasing MnSOD activity and decreasing age-associated mitochondrial reactive oxygen species accumulation.

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“…Moreover, it has been shown that HT inhibits proliferation of human MCF-7 breast cancer cells (Siriani et al, 2010;Bulotta et al 2011;Bouallagui et al, 2011a), human HT29 colon carcinoma cells (Guichard et al, 2006), human M14 melanoma cells (D'Angelo et al, 2005) and human PC3 prostate cells (Quiles et al, 2002).…”

Section: Anticancer Activitymentioning

confidence: 99%

Biological Properties of Hydroxytyrosol and Its Derivatives

Fernandez‐Bolanos¹,

López²,

López-García³

et al. 2012

Olive Oil - Constituents, Quality, Health Properties and Bioconversions

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Hydroxytyrosol, a natural antioxidant from olive oil, prevents protein damage induced by long-wave ultraviolet radiation in melanoma cells

Cited by 153 publications

References 70 publications

Cytotoxic and Apoptotic Effects of Hydroxytyrosol on Ovarian Cancer Cell Lines: Relationship of Cytotoxic Effect with ERK 1/2 Pathway

Cytotoxic and Apoptotic Effects of Hydroxytyrosol on Ovarian Cancer Cell Lines: Relationship of Cytotoxic Effect with ERK 1/2 Pathway

MnSOD activity regulates hydroxytyrosol-induced extension of chronological lifespan

Biological Properties of Hydroxytyrosol and Its Derivatives

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