Hydroxytyrosol, a phenolic compound from virgin olive oil, prevents macrophage activation

Maiuri, Maria Chiara; Stefano, Daniela De; Meglio, Paola Di; Irace, Carlo; Savarese, Maria Flavia; Sacchi, Raffaele; Cinelli, Mariapia; Carnuccio, Rosa

doi:10.1007/s00210-005-1078-y

Cited by 101 publications

(83 citation statements)

References 47 publications

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“…48) We previously reported that treatment of THP-1 cells with LPS 1 mg/ml induced NF-kB activation, which was prevented by pretreatment with HT 50 and 100 mM and NAC. The inhibitory effect of HT on NF-kB activation was consistent with of the reports of Maiuri et al 49) Indeed, the suppressive effect of LPS to NF-kB activation at HT 50 and 100 mM and NAC coincides with attenuated NO and ROS generation and elevated GSH content. Previous studies also observed antioxidant activity of HT.…”

Section: )supporting

confidence: 91%

Suppressive Effects of Hydroxytyrosol on Oxidative Stress and Nuclear Factor-κB Activation in THP-1 Cells

Zhang

Cao

Jiang

et al. 2009

Biological & Pharmaceutical Bulletin

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Recent publications have shown that an oxidized cytosolic environment amplifies activation of nuclear factor kappaB (NF-kB), which plays a critical role during inflammatory processes by activating many genes encoding for proinflammatory cytokines and immunoregulatory mediators.1) Some publications have also suggested that reactive oxygen species (ROS) influence inflammatory processes through mechanisms that are dependent on NF-kB.2) In the course of many inflammatory diseases reduced levels of glutathione (GSH) and/or increased levels of free radicals have been detected, 3,4) indicating the involvement of oxidative stress.Hydroxytyrosol (HT), present in the phenolic fraction of virgin olive oil in a concentration range of 10-300 ppm, is abundant in naturally fermented table olives and can be also recovered from virgin olive oil by-products. 5) At nutritionally relevant concentrations, HT possesses marked antioxidant activity and is a good radical scavenger.6,7) HT in vitro prevents protein damage in melanoma cells by ROS induced by long-wave ultraviolet light 8) and DNA damage in Jurkat cells exposed to hydrogen peroxide (H 2 O 2 ).9) Preincubation of intestinal Caco-2 cells with HT prevents the typical damages of oxidative stress. 10) Similarly, HT exerts protective effects against H 2 O 2 -induced oxidative hemolysis and malondialdehyde (MDA) formation in red blood cells.11) It also exerts inhibitory effects on GSH depletion induced by tert-butylhydroperoxide (t-BHP) in HepG2 cells.12) However, little information is available about the effects of HT on oxidative stress and NF-kB activation in LPS-stimulated THP-1 cells. In this study we assessed supression by HT of nitric oxide (NO), ROS, NF-kB activation, and the increase of GSH contents in lipopolysaccharide (LPS)-stimulated THP-1 cells. MATERIALS AND METHODS ChemicalsThe sample of HT was supplied by Eisai Food & Chemical Co., Ltd., Japan. LPS (Escherichia coli LPS, serotype 0127:B8), and 2Ј,7Ј-dichlorofluorescein diacetate (DCFH-DA) were purchased from Sigma (St. Louis, MO, U.S.A.).Cell Culture and Treatment Human monocytic THP-1 cells (American Type Culture Collection, Manassas, VA, U.S.A.) were obtained from Peking Union Medical College (Peking, China) and cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (Gibco), penicillin (100 U/ml; Gibco), and streptomycin (100 mg/ml; Gibco) at 37°C in a 5% CO 2 -humidified incubator. The cells were treated with or without the indicated concentrations of HT (25, 50, 100 mM) or N-acetylcysteine (NAC) (2.5 mM) for 10 min then incubated with LPS (1.0 mg/ml) for 3 h.Measurement of ROS Intracellular ROS production was measured using DCFH-DA by the method of Bass and coworkers, 13) with some modification. DCFH-DA penetrates the cells and is hydrolyzed by intracellular esterases to the nonfluorescent DCFH, which can be rapidly oxidized to the highly fluorescent 2,7-dichlorofluorescein (DCF) in the presence of ROS. THP-1 monocytes were inoculated into sixwell plates; the numbers of cells were 2ϫ10 5 cells/...

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Section: )supporting

confidence: 91%

Suppressive Effects of Hydroxytyrosol on Oxidative Stress and Nuclear Factor-κB Activation in THP-1 Cells

Zhang

Cao

Jiang

et al. 2009

Biological & Pharmaceutical Bulletin

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“…D'autre part, en ce qui concerne la lignée ostéoclastique (issue des macrophages), Moreno (2003) a démontré une inhibition du stress oxydant et de la production d'écosanoïdes in vitro par le β-sitostérol, le squalène et le tyrosol, via la modulation de la voie NF-κB. L'hydroxytyrosol exerce des effets similaires (Maiuri et al, 2005). En ce qui concerne le cartilage, l'oleuropéine empêche la production de TNF-α induite par LPS dans la lignée cellulaire monocytaire humaine THP-1, ce qui confirme le potentiel de cette molécule dans la prise en charge des pathologies ostéo-articulaires (Gong et al, 2012).…”

Section: Fraction Unsaponifiableunclassified

Huile d’olive et santé osseuse

Coxam

Wauquier

Darie

et al. 2014

OCL

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Résumé -L'ostéoporose représente un problème majeur de santé. La prise en charge classique de cette pathologie, essentiellement curative, est destinée aux personnes qui ont exprimé la maladie. Une telle stratégie ciblant la personne âgée souffrant d'ostéoporose sévère s'avère trop tardive. En fait, le traitement idéal de l'ostéoporose, pour être efficace, doit être oral, dénué d'effet secondaire, et surtout précoce de façon à inhiber très en amont le processus de perte osseuse. C'est pourquoi les stratégies de prévention visant à réduire les risques d'ostéoporose doivent être développées et instaurées. Ainsi, le concept d'une alimentation saine, fournissant des quantités suffisantes de différents nutriments potentiellement ostéoprotecteurs, mérite d'être mentionné. Dans cette optique, les olives ou l'huile d'olive vierge sont des aliments fonctionnels intéressants qui, en plus de leur richesse en acides gras mono-insaturés (acide oléique), contiennent plusieurs composants polyphénoliques ayant des propriétés biologiques spécifiques. Ils pourraient ainsi constituer des éléments efficaces pour prévenir l'ostéoporose, comme en témoignent les données de la littérature. Mots clés :Huile d'olive / polyphénols / acides gras / santé osseuse Abstract -Olive oil and bone health. Osteoporosis represents a major healthcare burden. Current pharmacologic approaches to osteoporosis are directed at those who already have the disease, which is often too late for the aged person with severe osteoporosis. Actually, the ideal therapy for to be effective for osteoporosis prevention needs to be oral, to be safe, and to inhibit bone loss as early as possible. This is why broad-based preventive strategies designed to lower the risks of osteoporosis need to be established and implemented. Thus, the concept of a healthy diet providing adequate amounts of various potent micronutrients deserves mention. In this light, olives or virgin olive oil are functional foods which besides having a high level of MUFA, the oleic acid, contains multiple minor phenolic components with specific biological properties. They could thus be a key component of successful dietary manipulations to prevent osteoporosis, as shown by many scientific publications.

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“…Maiuri et al [128] demonstrated that HTyr (100-200 FM) down-regulated, in a dose-dependent manner, the synthesis of PGE2 in LPS-stimulated J774 murine macrophages. Accordingly, Richard et al [124] showed that HTyr inhibited PGE2 production in LPS-stimulated RAW264.7 murine macrophages.…”

Section: In Vitro Htyr-mediated Anti-inflammatory Activity and Cancermentioning

confidence: 99%

Naturally Occurring Hydroxytyrosol: Synthesis and Anticancer Potential

Bernini¹,

Merendino²,

Romani³

et al. 2013

CMC

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Several epidemiological and animal studies have suggested that polyphenols, a group of secondary plant metabolites occurring mainly in the plant kingdom, may have a protective effect against some chronic degenerative diseases such as cancer. Polyphenols are part of the human diet, being present in vegetal food and beverages. Among them, an olive biophenol named hydroxytyrosol [2-(3,4-dihydroxyphenyl)ethanol, HTyr] has recently received particular attention because of its antioxidant, antiproliferative, pro-apoptotic, and anti-inflammatory activities, which have the potential to specifically counteract all cancer hallmarks, thus representing the expectant biological activities underlying the anti-tumor properties of this polyphenol. After a description of the synthetic procedures to prepare pure HTyr, this review takes into consideration the chemopreventive and chemotherapeutic potential of HTyr as the result of its antioxidant, antiproliferative and anti-inflammatory activities. In particular, the review is focused on the current knowledge of the main cellular and molecular mechanisms used by HTyr to affect carcinogenesis, highlighting the specific oncogenic and inflammatory signaling pathways potentially targeted by HTyr.

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Hydroxytyrosol, a phenolic compound from virgin olive oil, prevents macrophage activation

Cited by 101 publications

References 47 publications

Suppressive Effects of Hydroxytyrosol on Oxidative Stress and Nuclear Factor-κB Activation in THP-1 Cells

Suppressive Effects of Hydroxytyrosol on Oxidative Stress and Nuclear Factor-κB Activation in THP-1 Cells

Huile d’olive et santé osseuse

Naturally Occurring Hydroxytyrosol: Synthesis and Anticancer Potential

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Hydroxytyrosol, a phenolic compound from virgin olive oil, prevents macrophage activation

Cited by 101 publications

References 47 publications

Suppressive Effects of Hydroxytyrosol on Oxidative Stress and Nuclear Factor-&kappa;B Activation in THP-1 Cells

Suppressive Effects of Hydroxytyrosol on Oxidative Stress and Nuclear Factor-&kappa;B Activation in THP-1 Cells

Huile d’olive et santé osseuse

Naturally Occurring Hydroxytyrosol: Synthesis and Anticancer Potential

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Suppressive Effects of Hydroxytyrosol on Oxidative Stress and Nuclear Factor-κB Activation in THP-1 Cells

Suppressive Effects of Hydroxytyrosol on Oxidative Stress and Nuclear Factor-κB Activation in THP-1 Cells