HyfF subunit of hydrogenase 4 is crucial for regulating FOF1 dependent proton/potassium fluxes during fermentation of various concentrations of glucose

Vanyan, Liana; Trchounian, Karen

doi:10.1007/s10863-022-09930-x

Cited by 12 publications

(4 citation statements)

References 33 publications

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“…Observed differences may be related to sugar concentration‐dependent enzyme activities and interactions 45 as recently new glucose concentration‐dependent H 2 producing model consisting of different subunits of Hyd‐3 and Hyd‐4 has been suggested 32 . Moreover, lately, it has been shown that some subunits of Hyd‐4 play a key role in glucose sensing for regulating DCCD‐sensitive H + /K + fluxes 46 …”

Section: Resultsmentioning

confidence: 99%

“…32 Moreover, lately, it has been shown that some subunits of Hyd-4 play a key role in glucose sensing for regulating DCCD-sensitive H + /K + fluxes. 46 Different data were obtained when longer hydrolysis was applied: H 2 production was determined in the presence of all the tested 20 to 200 g L À1 CS containing mediums with different yields (Figure 3A). When E. coli wild type was grown in medium containing initially 20 g L À1 CS with or without dilution H 2 production started at 24 h and lasted 72 h, which significantly differs from shorter hydrolyzed samples.…”

Section: Hydrogen Production During Batch Fermentation Of Csmentioning

confidence: 97%

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Coffee silverskin as a substrate for biobased production of biomass and hydrogen by Escherichia coli

Mirzoyan

Aghekyan

Vanyan

et al. 2022

Intl J of Energy Research

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Currently, coffee consumption reached 9.92 million tons, being responsible for the generation of huge amounts of waste associated with coffee bean processing steps and further operations. Coffee silverskin (CS) and spent coffee grounds having high organic content are generated as a result of roasting and brewing processes. Escherichia coli wild-type and septuple mutant (ΔhyaB ΔhybC ΔhycA ΔfdoG ΔldhA ΔfrdC ΔaceE) growth and hydrogen production were investigated in mediums containing 20 to 200 g L À1 CS with dilutions, hydrolyzed for 25 or 45 min. During utilization of CS, hydrolysate phenolic and flavonoid content does affect bacterial growth, moreover, shorter hydrolyzing was more optimal for biomass generation. Highest specific growth rate of 0.64 ± 0.02 h À1 was observed in wild-type cells when 5 times diluted 200 g L À1 CS was applied to yield 0.495 ± 0.015 g L À1 biomass. Prolonged

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Section: Resultsmentioning

confidence: 99%

Section: Hydrogen Production During Batch Fermentation Of Csmentioning

confidence: 97%

Coffee silverskin as a substrate for biobased production of biomass and hydrogen by Escherichia coli

Mirzoyan

Aghekyan

Vanyan

et al. 2022

Intl J of Energy Research

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“…Hyd-1 and Hyd-2 can work either in oxidizing or in producing mode depending on external pH and carbon source [8] . Hyd-3 encoded by hyc operon with formate dehydrogenase H (FDH-H) form formate hydrogen lyase (FHL-1) complex while Hyd-4 encoded by hyf operon forms FHL-2 complex [9] – [11] . During glucose fermentation, Hyd-3 is a major H 2 producing Hyd enzyme, while Hyd-4 is mainly responsible for H 2 uptake or, at pH 5.5 together with Hyd-3, forms a newly suggested H 2 producing Hyd complex [12] , [13] .…”

Section: Introductionmentioning

confidence: 99%

Osmotic stress as a factor for regulating E. coli hydrogenase activity and enhancing H2 production during mixed carbon sources fermentation

Babayan,

Vassilian,

Trchounian

2023

AIMSMICRO

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<abstract> <italic>Escherichia coli</italic> performs mixed-acid fermentation and produces molecular hydrogen (H2) via reversible hydrogenases (Hyd). H2 producing activity was investigated during hyper- and hypo-osmotic stress conditions when a mixture of carbon sources (glucose and glycerol) was fermented at different pHs. Hyper-osmotic stress decreased H2 production rate (VH2) ~30 % in wild type at pH 7.5 when glucose was supplemented, while addition of formate stimulated VH2 ~45% compared to hypo-stress conditions. Only in <italic>hyfG</italic> in formate assays was VH2 inhibited ~25% compared to hypo-stress conditions. In hypo-stress conditions addition of glycerol increased VH2 ~2 and 3 fold in <italic>hybC</italic> and <italic>hyfG</italic> mutants, respectively, compared to wild type. At pH 6.5 hyper-osmotic stress stimulated VH2 ~2 fold in all strains except <italic>hyaB</italic> mutant when glucose was supplemented, while in formate assays significant stimulation (~3 fold) was determined in <italic>hybC</italic> mutant. At pH 5.5 hyper-osmotic stress inhibited VH2 ~30% in wild type when glucose was supplemented, but in formate assays it was stimulated in all strains except <italic>hyfG</italic>. Taken together, it can be concluded that, depending on external pH and absence of Hyd enzymes in stationary-phase-grown osmotically stressed <italic>E. coli</italic> cells, H2 production can be stimulated significantly which can be applied in developing H2 production biotechnology. </abstract>

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“…In another example of cellular response to glucose availability, it has recently been reported that hydrogenase 4 subunits HyfBDF can sense two different glucose concentrations (2 or 8 g/L). Depending on the amount of glucose in the medium, hydrogenase 4 can regulate the H + /K + fluxes to maintain proton motive force generation [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Glucose consumption rate-dependent transcriptome profiling of Escherichia coli provides insight on performance as microbial factories

et al. 2022

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Background The modification of glucose import capacity is an engineering strategy that has been shown to improve the characteristics of Escherichia coli as a microbial factory. A reduction in glucose import capacity can have a positive effect on production strain performance, however, this is not always the case. In this study, E. coli W3110 and a group of four isogenic derivative strains, harboring single or multiple deletions of genes encoding phosphoenolpyruvate:sugar phosphotransferase system (PTS)-dependent transporters as well as non-PTS transporters were characterized by determining their transcriptomic response to reduced glucose import capacity. Results These strains were grown in bioreactors with M9 mineral salts medium containing 20 g/L of glucose, where they displayed specific growth rates ranging from 0.67 to 0.27 h−1, and specific glucose consumption rates (qs) ranging from 1.78 to 0.37 g/g h. RNA-seq analysis revealed a transcriptional response consistent with carbon source limitation among all the mutant strains, involving functions related to transport and metabolism of alternate carbon sources and characterized by a decrease in genes encoding glycolytic enzymes and an increase in gluconeogenic functions. A total of 107 and 185 genes displayed positive and negative correlations with qs, respectively. Functions displaying positive correlation included energy generation, amino acid biosynthesis, and sugar import. Conclusion Changes in gene expression of E. coli strains with impaired glucose import capacity could be correlated with qs values and this allowed an inference of the physiological state of each mutant. In strains with lower qs values, a gene expression pattern is consistent with energy limitation and entry into the stationary phase. This physiological state could explain why these strains display a lower capacity to produce recombinant protein, even when they show very low rates of acetate production. The comparison of the transcriptomes of the engineered strains employed as microbial factories is an effective approach for identifying favorable phenotypes with the potential to improve the synthesis of biotechnological products.

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HyfF subunit of hydrogenase 4 is crucial for regulating FOF1 dependent proton/potassium fluxes during fermentation of various concentrations of glucose

Cited by 12 publications

References 33 publications

Coffee silverskin as a substrate for biobased production of biomass and hydrogen by Escherichia coli

Coffee silverskin as a substrate for biobased production of biomass and hydrogen by Escherichia coli

Osmotic stress as a factor for regulating <i>E. coli</i> hydrogenase activity and enhancing H<sub>2</sub> production during mixed carbon sources fermentation

Glucose consumption rate-dependent transcriptome profiling of Escherichia coli provides insight on performance as microbial factories

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