HYL1 controls the miR156-mediated juvenile phase of vegetative growth

Li, Shuxia; Yang, Xi; Wu, Feijie; He, Yuqing

doi:10.1093/jxb/err465

Cited by 37 publications

(29 citation statements)

References 40 publications

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“…Loss of function or gain of function of DOG1 decreases or increases, respectively, expression of DICER-related genes (Fig. 4 E-G), and previous studies found that miR156 and miR172 levels are altered in mutants of DCL1, HYL1, SE, TGH, and CDC5 (42,56,58,62,63). However, multiple feedback loops are associated with transcription and processing of miRNAs (41), so further study is needed to clarify the specific mechanism by which DOG1 affects miRNA levels.…”

Section: Discussionmentioning

confidence: 80%

“…Interestingly, the delayed-flowering phenotype of dcl1-7 mutant could be rescued by expression of a DCL4-dependent MIR839 in which the mature sequence was replaced by the one encoding MIR172, suggesting that the delayed flowering in dcl1-7 plants is a result of the reduction in miR172 (56). Earlier transition from the juvenile to the adult phase was also observed in loss-of-function mutants of SE and HYL1, in association with substantial reduction in miR156, and could be rescued by overexpression of MIR156 (57,58). Another miR156 target, SPL13, plays an important role in the regulation of the postgerminative switch from the cotyledon stage to the vegetative-leaf stage during seedling growth (59).…”

Section: Discussionmentioning

confidence: 93%

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DELAY OF GERMINATION1 ( DOG1 ) regulates both seed dormancy and flowering time through microRNA pathways

Huo

Wei

Bradford

2016

Proc. Natl. Acad. Sci. U.S.A.

166

149

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Seed germination and flowering, two critical developmental transitions in plant life cycles, are coordinately regulated by genetic and environmental factors to match plant establishment and reproduction to seasonal cues. The DELAY OF GERMINATION1 (DOG1) gene is involved in regulating seed dormancy in response to temperature and has also been associated genetically with pleiotropic flowering phenotypes across diverse Arabidopsis thaliana accessions and locations. Here we show that DOG1 can regulate seed dormancy and flowering times in lettuce (Lactuca sativa, Ls) and Arabidopsis through an influence on levels of microRNAs (miRNAs) miR156 and miR172. In lettuce, suppression of LsDOG1 expression enabled seed germination at high temperature and promoted early flowering in association with reduced miR156 and increased miR172 levels. In Arabidopsis, higher miR156 levels resulting from overexpression of the MIR156 gene enhanced seed dormancy and delayed flowering. These phenotypic effects, as well as conversion of MIR156 transcripts to miR156, were compromised in DOG1 loss-of-function mutant plants, especially in seeds. Overexpression of MIR172 reduced seed dormancy and promoted early flowering in Arabidopsis, and the effect on flowering required functional DOG1. Transcript levels of several genes associated with miRNA processing were consistently lower in dry seeds of Arabidopsis and lettuce when DOG1 was mutated or its expression was reduced; in contrast, transcript levels of these genes were elevated in a DOG1 gain-of-function mutant. Our results reveal a previously unknown linkage between two critical developmental phase transitions in the plant life cycle through a DOG1-miR156-miR172 interaction.T he life cycles of flowering plants are characterized by distinct phase transitions such as from seed to seedling (germination) or from vegetative to reproductive development (flowering) (1). The timing of germination and flowering both require precise environmental sensing and integrated responses to multiple inputs so that developmental transitions can be accurately matched to seasonal conditions (1-3). Seeds use temperature as a signal of the seasonal and current environmental conditions to determine opportune times to germinate with respect to the potential for seedling survival (2, 4, 5). Similarly, in many plants the transition from vegetative to floral development occurs in response to environmental cues, particularly temperature and day length (6, 7). Ecological and evolutionary studies have found that seed germination and flowering traits within species are coadapted across habitat ranges (8-11). Seed dormancy and germination are regulated primarily by the antagonistic actions of the plant hormones gibberellin (GA; promotive) and abscisic acid (ABA; inhibitory), whose synthesis and action vary in response to environmental signals (12). Recent studies indicate that canonical genes regulating flowering, such as FLOWERING LOCUS T (FT) and FLOWERING LOCUS C (FLC), are also involved in the transition from seed dormanc...

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Section: Discussionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 93%

DELAY OF GERMINATION1 ( DOG1 ) regulates both seed dormancy and flowering time through microRNA pathways

Huo

Wei

Bradford

2016

Proc. Natl. Acad. Sci. U.S.A.

166

149

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“…These results indicate that the appropriate miR156 level is necessary and sufficient for maintenance of the juvenile phase. Genetic studies have also shown that mutants precociously exhibiting adult characteristics have low miR156 expression, whereas mutants with an elongated juvenile phase overexpress miR156 (Smith et al, 2009;Li et al, 2012;Chuck et al, 2007;Tanaka et al, 2011). Such studies support the importance of miR156 levels in maintaining the juvenile phase.…”

Section: Introductionmentioning

confidence: 65%

Jasmonate regulates juvenile-adult phase transition in rice

et al. 2016

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Juvenile-to-adult phase transition is an important shift for the acquisition of adult vegetative characteristics and subsequent reproductive competence. We identified a recessive precocious ( pre) mutant exhibiting a long leaf phenotype in rice. The long leaf phenotype is conspicuous in the second to the fourth leaves, which are juvenile and juvenile-to-adult transition leaves. We found that morphological and physiological traits, such as midrib formation, shoot meristem size, photosynthetic rate and plastochron, in juvenile and juvenile-to-adult transition stages of the pre mutant have precociously acquired adult characteristics. In agreement with these results, expression patterns of miR156 and miR172, which are microRNAs regulating phase change, support the accelerated juvenile-to-adult phase change in the pre mutant. The mutated gene encodes an allene oxide synthase (OsAOS1), which is a key enzyme for the biosynthesis of jasmonic acid (JA). The pre mutant showed a low level of JA and enhanced sensitivity to gibberellic acid, which promotes the phase change in some plant species. We also show that prolonged plastochron in the pre mutant is caused by accelerated PLASTOCHRON1 (PLA1) function. The present study reveals a substantial role of JA as a negative regulator of vegetative phase change.

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“…In Arabidopsis , number of the rosette leaves was recorded at flowering stage, according to the method of Li et al . (). More than three independent transgenic lines were observed.…”

Section: Methodsmentioning

confidence: 97%

Tuning growth cycles of Brassica crops via natural antisense transcripts of BrFLC

Zhang

Bai

et al. 2015

Plant Biotechnology Journal

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SummarySeveral oilseed and vegetable crops of Brassica are biennials that require a prolonged winter cold for flowering, a process called vernalization. FLOWERING LOCUS C (FLC) is a central repressor of flowering. Here, we report that the overexpression of natural antisense transcripts (NATs) of Brassica rapa FLC (BrFLC) greatly shortens plant growth cycles. In rapid-, medium-and slowcycling crop types, there are four copies of the BrFLC genes, which show extensive variation in sequences and expression levels. In Bre, a biennial crop type that requires vernalization, five NATs derived from the BrFLC2 locus are rapidly induced under cold conditions, while all four BrFLC genes are gradually down-regulated. The transgenic Bre lines overexpressing a long NAT of BrFLC2 do not require vernalization, resulting in a gradient of shortened growth cycles. Among them, a subset of lines both flower and set seeds as early as Yellow sarson, an annual crop type in which all four BrFLC genes have non-sense mutations and are nonfunctional in flowering repression. Our results demonstrate that the growth cycles of biennial crops of Brassica can be altered by changing the expression levels of BrFLC2 NATs. Thus, BrFLC2 NATs and their transgenic lines are useful for the genetic manipulation of crop growth cycles.

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HYL1 controls the miR156-mediated juvenile phase of vegetative growth

Cited by 37 publications

References 40 publications

DELAY OF GERMINATION1 ( DOG1 ) regulates both seed dormancy and flowering time through microRNA pathways

DELAY OF GERMINATION1 ( DOG1 ) regulates both seed dormancy and flowering time through microRNA pathways

Jasmonate regulates juvenile-adult phase transition in rice

Tuning growth cycles of Brassica crops via natural antisense transcripts of BrFLC

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