Hymenobacter rubripertinctus sp. nov., isolated from Antarctic tundra soil

Abstract

A red-pigmented, Gram-reaction-negative, aerobic, non-motile and rod-shaped bacterium, designated NY03-3-30, was isolated from a soil sample collected from Inexpressible Island, Northern Victoria Land of the Antarctic Ross Orogen, and subjected to a polyphasic taxonomic study. Growth occurred at 4-28 °C (optimum 20 °C) and at pH 6.0-9.0 (optimum pH 7.0). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NY03-3-30 belonged to the genus Hymenobacter in the family Cytophagaceae. 16S rRNA gene sequence similarities between strain NY03-3-30 and the type strains of Hymenobacter species with validly published names ranged from 92.7 to 96.2 %. Strain NY03-3-30 contained summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0, C16 : 0, C16 : 1ω5c, anteiso-C15 : 0 and summed feature 4 (iso-C17 : 1-I and/or anteiso-C17 : 1-B) as major cellular fatty acids, MK-7 as the respiratory quinone and phosphatidylethanolamine as the main polar lipid. The DNA G+C content of strain NY03-3-30 was 59.4 mol%. On the basis of phylogenetic, physiological and chemotaxonomic data, strain NY03-3-30 is considered to represent a novel species of genus Hymenobacter, for which the name Hymenobacter rubripertinctus sp. nov. is proposed. The type strain is NY03-3-30 (=CCTCC AB 2017095=KCTC 62163).

Hymenobacter sediminis sp. nov., isolated from lake sediment

“…Strain ELS1360 T contained MK-7 as the sole respiratory quinone, which is consistent with other species of the genus Hymenobacter [7]. The G+C content of the bacterium, calculated from the draft genome sequence, was 57.1 mol%, which lies within the range observed for recognized members of the genus Hymenobacter [8].…”

“…By August 2019, the genus comprised 67 species with validly published names according to the List of Prokaryotic Names with Standing in Nomenclature (LPSN) [6]. Members of the genus Hymenobacter have been isolated from a variety of environmental samples, such as soil, arctic tundra soil, stream sediment, spring, freshwater, seawater and wastewater [7][8][9][10][11][12][13]. NaCl tolerance of most members of Hymenobacter is <2 % (w/v) [8][9][10][11][12][13].…”

“…Members of the genus Hymenobacter have been isolated from a variety of environmental samples, such as soil, arctic tundra soil, stream sediment, spring, freshwater, seawater and wastewater [7][8][9][10][11][12][13]. NaCl tolerance of most members of Hymenobacter is <2 % (w/v) [8][9][10][11][12][13]. All species have Gram-stain-negative, rod-shaped cells that form pink to red-pigmented colonies, contain menaquinone-7 (MK-7) as the major respiratory quinone, possess iso-C 15 : 0 , anteiso-C 15 : 0 , summed feature 3 and summed feature 4 as the major fatty acids and include phosphatidylethanolamine as the major polar lipid [7].…”

Antarctic heterotrophic bacterium Hymenobacter nivis P3T displays light‐enhanced growth and expresses putative photoactive proteins

“…Heterotrophic bacteria belonging to the genus Hymenobacter , which is a member of the Bacteroidetes phylum, have been isolated from polar environments with high irradiance such as ice, snow, rocks and soil, from the Arctic and Antarctica (Klassen and Foght, ; Koo et al ., ; Ahn et al ., ; Kojima et al ., ; Oh et al ., ; Ordenes‐Aenishanslins et al ., ; Sedlacek et al ., ; Jiang et al ., ). Interestingly, Hymenobacter species have been isolated from algae‐rich environments such as lichen and red‐coloured snow, suggesting that these bacteria are able to withstand environments with high light exposure although they are not photosynthetic (Fujii et al ., ; Ahn et al ., ; Kojima et al ., ; Oh et al ., ; Sedlacek et al ., ).…”

Hymenobacter terrestris sp. nov. and Hymenobacter lapidiphilus sp. nov., isolated from regoliths in Antarctica

“…Evolutionary analyses were conducted in mega software version 10 [48]. NZ_QVLT00000000) [15], H. aerophilus DSM 13606 T (NZ_ ARNJ00000000) [29], H. psychrophilus CGMCC 1.8975 T (NZ_FNOV00000000) [30], H. actinosclerus CCUG 39621 T (NZ_FOHS01000001) [31], H. amundsenii CCM 8682 T (NZ_NIRR00000000) [16] and H. rubripertinctus NY03-3-30 T (NZ_QYCN00000000) [14] were obtained from the NCBI database. The ANI values calculated between P5252 T , P5342 T and the phylogenetically closest species H. humicola, H. aerophilus, H. psychrophilus, H. actinosclerus, H. amundsenii and H. rubripertinctus are listed in Table S1 and were well below the proposed cut-off values (95-96%) recommended for species delineation [24].…”