Hymenobacter siberiensis sp. nov., isolated from a marine sediment of the East Siberian Sea and Hymenobacter psoromatis sp. nov., isolated from an Antarctic lichen

Abstract

Gram-stain-negative, strictly aerobic, red-pink-coloured, rod-shaped and non-motile bacterial strains PAMC 29290, PAMC 29294T and PAMC 29296 were isolated from marine surface sediment sampled in the East Siberian Sea and strains PAMC 26553 and PAMC 26554T were obtained from an Antarctic lichen. Strains PAMC 29290, PAMC 29294T and PAMC 29296 were closely related to Hymenobacter artigasi (98.8 % 16S rRNA gene similarity), Hymenobacter antarcticus (97.3 %) and Hymenobacter glaciei (96.9 %), and PAMC 26553 and PAMC 26554T showed high similarity to Hymenobacter ginsengisoli (97.0 %), Hymenobacter rivuli (96.1 %) and Hymenobacter setariae (95.9 %). Genomic relatedness analyses showed that strains PAMC 29290, PAMC 29294T and PAMC 29296 could be distinguished from H. artigasi by average nucleotide identity (ANI; 93.1–93.2 %) and digital DNA–DNA hybridization (dDDH; 50.3–51.0 %) values. Strains PAMC 26553 and PAMC 26554T could be clearly distinguished from H. ginsengisoli with ANI values <79.8 % and dDDH values <23.3 %. The major fatty acids of strains PAMC 29290, PAMC 29294T and PAMC 29296 were C15 : 0 iso (21.0–26.0 %), summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c; 17.4–18.2 %), C15 : 0 anteiso (12.7–19.1 %) and summed feature 4 (C17 : 1 iso I and/or anteiso B; 8.6–16.1 %) and those of strains PAMC 26553 and PAMC 26554T were summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c; 20.7–22.2 %), C15 : 0 anteiso (17.5–19.7 %) and summed feature 4 (C17 : 1 iso I and/or anteiso B; 15.5–18.1 %). The major respiratory quinone was MK-7. The genomic DNA G+C contents were 60.6–60.8 mol%. The polar lipids of PAMC 29294T were found to consist of phosphatidylethanolamine, four unidentified aminolipids, an unidentified aminophospholipid and five unidentified lipids; those of PAMC 26554T were phosphatidylethanolamine, three unidentified aminolipids, four unidentified aminophospholipid and two unidentified lipids. The distinct phylogenetic position and some physiological characteristics distinguished the novel strains from closely related type strains in the genus Hymenobacter . Thus, two novel species are proposed, with the names Hymenobacter siberiensis sp. nov. (type strain, PAMC 29294T=KCTC 82466T=JCM 34574T) and Hymenobacter psoromatis sp. nov. (type strain, PAMC 26554T=KCTC 82464T=JCM 34572T), respectively.

Hymenobacter canadensis sp. nov., isolated from freshwater of the pond in Cambridge Bay, Canada

“…The bacterial strain PAMC 29467 T was isolated from freshwater of the pond in Cambridge bay, Canada during a study on the physiological characterization of culturable bacteria from Arctic freshwater. Out of the 100 type strains of the genus Hymenobacter, 22 strains were obtained from air [2], glacier [5], lichens [2], marine sediment [1], red snow [1], rocks [4], and soils [7] of Antarctica or Arctic [1,6,7,13,16,20,36,40,53,64,72,79]. Thirteen strains of the genus Hymenobacter have been isolated from freshwater samples collected in Republic of Korea and Taiwan.…”

“…The genus Hymenobacter encompassed 100 validly published species on LPSN (https://lpsn.dsmz.de/genus/hymenobacter) at the time of writing (December 2022) [2]. One hundred type strains of the genus Hymenobacter have been isolated from various samples including air, freshwater, freshwater sediment, glaciers, lichen, marine sediment, plant, sand, seawater, and soil collected in tropical to polar regions, wastewater and meat, implying that members of the genus Hymenobacter are successful colonisers in a wide range of environmental conditions (Table S1) [1, 3–84]. Type strains of the genus Hymenobacter are characterized as aerobic, rod-shaped, red-pink or pink-pigmented, Gram-stain-negative, and heterotrophic [6, 13, 20, 30].…”

“…Type strains of the genus Hymenobacter are characterized as aerobic, rod-shaped, red-pink or pink-pigmented, Gram-stain-negative, and heterotrophic [6, 13, 20, 30]. In addition, most of the type strains grow at and lower than 10 °C and some strains such as Hymenobacter amundsenii , Hymenobacter artigasi , Hymenobacter coccineus , Hymenobacter glacialis , Hymenobacter ginsengisoli , Hymenobacter lapidarius , Hymenobacter kanuolensis , Hymenobacter rubidus , Hymenobacter psoromatis , Hymenobacter siberiensis , Hymenobacter terrae , and Hymenobacter tibetensis are resistant to UV irradiation (Table S1, available in the online version of this article) [7, 13, 20, 32, 38, 63, 72, 77, 81].…”

Hymenobacter endophyticus sp. nov., isolated from wheat leaf tissue

“…As a result, Hymenobacter species frequently exhibit resistance to UV, cold temperatures and heavy metals. So far, a few Hymenobacter species have been obtained from plant tissues, including the bark of Ginkgo biloba [21], dry spikelet clusters of grass Setaria viridis [22] and Antarctic lichen [23]. In our previous study, Hymenobacter was found to be one of the dominant taxa in the microbial community of wheat leaves [24].…”