Hymenobacter terricola sp. nov., isolated from Antarctic soil

Abstract

Strain 3F2T was isolated from a soil sample obtained from the surface of Deception Island, Antarctica. The isolate was a Gram-stain-negative, aerobic, non-motile, rod-shaped bacterium, and its colonies were red to pink in colour. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3F2T belonged to the genus Hymenobacter , family Hymenobacteraceae and was most closely related to Hymenobacter sedentarius DG5BT (97.0% sequence similarity), Hymenobacter soli PB17T (96.9%), Hymenobacter terrae DG7AT (96.8%) and Hymenobacter rufus S1-2-2-6T (96.5%). Growth occurred at 4–20 °C (optimum, 10 °C), up to 1.0 % (w/v) NaCl (optimum, 0%) and pH 6.0–8.0 (optimum, pH 7.0). The chemotaxonomic characteristics of strain 3F2T, which had MK-7 as its predominant menaquinone and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), iso-C15:0, anteiso-C15:0 and C16:1 ω5c as its major fatty acids, were consistent with classification in the genus Hymenobacter . The polar lipid profile of strain 3F2T comprised phosphatidylethanolamine, two unidentified aminolipids, two unidentified aminophospholipids and three unidentified polar lipids. The genome of strain 3F2T was 6.56 Mbp with a G+C content of 61.5 mol%. Average nucleotide identity (ANI) values between 3F2T and the other species of the genus Hymenobacter were found to be low (ANIm <87.0%, ANIb <82.0% and OrthoANIu <83.0%). Furthermore, digital DNA–DNA hybridization and average amino acid identity values between strain 3F2T and the closely related species ranged from 20.0 to 26.3% and from 64.0 to 81.1 %, respectively. Based on the results of our phylogenetic, phenotypic, genotypic and chemotaxonomic analyses, it is concluded that strain 3F2T represents a novel species within the genus Hymenobacter , for which the name Hymenobacter terricola sp. nov. is proposed. The type strain is 3F2T (=KCTC 72468T=CGMCC 1.13716T).

Hymenobacter canadensis sp. nov., isolated from freshwater of the pond in Cambridge Bay, Canada

“…The genus Hymenobacter encompassed 100 validly published species on LPSN (https://lpsn.dsmz.de/genus/hymenobacter) at the time of writing (December 2022) [2]. One hundred type strains of the genus Hymenobacter have been isolated from various samples including air, freshwater, freshwater sediment, glaciers, lichen, marine sediment, plant, sand, seawater, and soil collected in tropical to polar regions, wastewater and meat, implying that members of the genus Hymenobacter are successful colonisers in a wide range of environmental conditions (Table S1) [1, 3–84]. Type strains of the genus Hymenobacter are characterized as aerobic, rod-shaped, red-pink or pink-pigmented, Gram-stain-negative, and heterotrophic [6, 13, 20, 30].…”

“…The bacterial strain PAMC 29467 T was isolated from freshwater of the pond in Cambridge bay, Canada during a study on the physiological characterization of culturable bacteria from Arctic freshwater. Out of the 100 type strains of the genus Hymenobacter, 22 strains were obtained from air [2], glacier [5], lichens [2], marine sediment [1], red snow [1], rocks [4], and soils [7] of Antarctica or Arctic [1,6,7,13,16,20,36,40,53,64,72,79]. Thirteen strains of the genus Hymenobacter have been isolated from freshwater samples collected in Republic of Korea and Taiwan.…”

Hymenobacter endophyticus sp. nov., isolated from wheat leaf tissue

“…So far, 102 species of this genus with validly published names have been reported based on the LPSN [2]. Many Hymenobacter species were isolated from adverse environments, including areas with high levels of radiation [3], the Arctic [4,5], the Antarctic [6][7][8][9][10][11][12], a lead-zinc mine [13,14], deep sea water [15], glaciers [16][17][18] and deserts [19,20]. As a result, Hymenobacter species frequently exhibit resistance to UV, cold temperatures and heavy metals.…”