Hyperaminoacidaemia at postprandial levels does not modulate glucose metabolism in type 2 diabetes mellitus

Bassil, Maya; Burgos, Sergio A.; Marliss, Errol B.; Morais, José A.; Chevalier, Stéphanie; Gougeon, Réjeanne

doi:10.1007/s00125-011-2115-7

Cited by 19 publications

(9 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our data on plasma BCAA showed that when leucine gavage is administered prior to ITT, the elevated plasma BCAA is rapidly brought back to normal, consistent with the idea that in lean rats, these amino acids do not impair insulin sensitivity, as others have shown (Bassil et al. ). Obviously this measure reflects whole body average and does not allow identification of tissue‐specific effect.…”

Section: Discussionsupporting

confidence: 89%

“…impaired insulin regulation of protein metabolism, which would lead to increased availability of free (glucogenic) AA, to the increased gluconeogenesis observed in insulinresistant individuals (Chevalier et al 2006). Our data on plasma BCAA showed that when leucine gavage is administered prior to ITT, the elevated plasma BCAA is rapidly brought back to normal, consistent with the idea that in lean rats, these amino acids do not impair insulin sensitivity, as others have shown (Bassil et al 2011). Obviously this measure reflects whole body average and does not allow identification of tissue-specific effect.…”

Section: Discussionsupporting

confidence: 89%

See 1 more Smart Citation

Amino acid-induced impairment of insulin sensitivity in healthy and obese rats is reversible

et al. 2014

View full text Add to dashboard Cite

High‐protein diets (HPDs) promote weight loss but other studies implicate these diets and their constituent amino acids (AAs) in insulin resistance. We hypothesized that AA‐induced insulin resistance is a temporal and reversible metabolic event. L6 myotubes were serum deprived for 4 h and then incubated in AA and/or insulin (100 nmol/L). Another group of cells was incubated overnight in AA + insulin, starved again, and then reincubated with AA and insulin. Mammalian (mechanistic) target of rapamycin complex 1 (mTORC1) signaling and glucose uptake were then measured. Healthy or insulin‐resistant rats were gavaged with leucine (0.48 g/kg) and insulin sensitivity was examined. In myotubes, incubation with AA and insulin significantly (P <0.05) increased the phosphorylation of the mTORC1 substrate ribosomal protein S6 kinase 1 (S6K1, T389) and of insulin receptor substrate 1 (IRS‐1, serine residues), but suppressed insulin‐stimulated glucose uptake by 40% (P <0.01). These modifications were mTORC1‐dependent and were reversible. In vivo, leucine gavage reversibly increased S6K1 phosphorylation and IRS‐1 serine phosphorylation 5‐ to 12‐fold in skeletal muscle and impaired insulin tolerance of glucose (P <0.05) in lean rats. In insulin‐resistant rats, the impairment of whole blood glucose and AA metabolism induced by leucine gavage (0.001 < P <0.05) was more severe than that observed in lean rats; however, the impairment was reversible within 24 h of treatment. If these data are confirmed in long‐term studies, it would imply that the use of leucine/HPD in treating metabolic diseases is unlikely to have lasting negative effects on insulin sensitivity.

show abstract

Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 89%

Amino acid-induced impairment of insulin sensitivity in healthy and obese rats is reversible

et al. 2014

View full text Add to dashboard Cite

show abstract

“…These authors have also reported that the anabolic response of skeletal muscle protein metabolism to insulin infusion is very similar in diabetic and healthy subjects [128,129]. On the other hand, other authors have described that insulin resistance is involved in protein metabolism [130,131]. More specifically, they have shown that, upon insulin infusion, type 2 diabetic subjects do not display increased protein anabolism or inhibition of protein catabolism.…”

Section: Protein Metabolism and Muscle Mass In Type 2 Diabetes Mellitmentioning

confidence: 71%

Differential control of muscle mass in type 1 and type 2 diabetes mellitus

Sala

Zorzano

2015

Cell. Mol. Life Sci.

View full text Add to dashboard Cite

Diabetes mellitus--whether driven by insulin deficiency or insulin resistance--causes major alterations in muscle metabolism. These alterations have an impact on nutrient handling, including the metabolism of glucose, lipids, and amino acids, and also on muscle mass and strength. However, the ways in which the distinct forms of diabetes affect muscle mass differ greatly. The most common forms of diabetes mellitus are type 1 and type 2. Thus, whereas type 1 diabetic subjects without insulin treatment display a dramatic loss of muscle, most type 2 diabetic subjects show no changes or even an increase in muscle mass. However, the most commonly used rodent models of type 2 diabetes are characterized by muscle atrophy and do not mimic the features of the disease in humans in terms of muscle mass. In this review, we analyze the processes that are differentially regulated under these forms of diabetes and propose regulatory mechanisms to explain them.

show abstract

“…We showed that amino acids can overcome the insulin resistance of protein metabolism through their anabolic action (Bassil et al, 2011a) without further impairing glucose metabolism in obese middle-aged men with type 2 diabetes (Bassil et al, 2011b). Moreover, a recent systematic review and meta-analysis of 20 randomized controlled trials investigating dietary approaches in the management of type 2 diabetes indicated that high-protein diets are among the few approaches that are effective in improving glycemic control (Ajala et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Effect of obesity and type 2 diabetes on protein anabolic response to insulin in elderly women

Murphy

Chevalier

Gougeon

et al. 2015

Experimental Gerontology

View full text Add to dashboard Cite

Hyperaminoacidaemia at postprandial levels does not modulate glucose metabolism in type 2 diabetes mellitus

Abstract: Postprandial circulating amino acid concentrations do not worsen the already attenuated glucose disposal in hyperglycaemic type 2 diabetic men, and cell-signalling events are consistent with this. Our results do not support recommendations to restrict dietary protein in type 2 diabetes.

Cited by 19 publications

References 42 publications

Amino acid-induced impairment of insulin sensitivity in healthy and obese rats is reversible

Amino acid-induced impairment of insulin sensitivity in healthy and obese rats is reversible

Differential control of muscle mass in type 1 and type 2 diabetes mellitus

Effect of obesity and type 2 diabetes on protein anabolic response to insulin in elderly women

Contact Info

Product

Resources

About