Hyperbaric oxygen induces a cytoprotective and angiogenic response in human microvascular endothelial cells

Godman, Cassandra A.; Chheda, Kousanee P.; Hightower, Lawrence E.; Perdrizet, George A.; Shin, Dong-Guk; Giardina, Charles

doi:10.1007/s12192-009-0159-0

Cited by 122 publications

(105 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Hyperbaric oxygen treatment (HBOT) seems to have beneficial effects on wound healing and reparative angiogenesis [73]. Global gene expression analysis on human microvascular endothelial cells exposed to hyperbaric oxygen under conditions similar to a clinical treatment showed that some metallothioneins especially MT-1E, MT-1F, MT-1G, MT-1H, MT-1M and MT-1X were up-regulated immediately after HBOT, some metallothioneins as MT-1E, MT-1F, MT-1H and MT-1X after 24 h [73].…”

Section: Cardiovascular Diseasesmentioning

confidence: 99%

Microarray analysis of metallothioneins in human diseases—A review

Křížková

Kępińska

Emri

et al. 2016

Journal of Pharmaceutical and Biomedical Analysis

View full text Add to dashboard Cite

This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. 1 Microarray analysis of metallothioneins in human diseases AbstractMetallothioneins (MTs), low molecular mass cysteine-rich proteins, which are able to bind up to 20 monovalent and up to 7 divalent heavy metal ions are widely studied due to their functions in detoxification of metals, scavenging free radicals and cells protection against the oxidative stress. It was found that the loss of the protective effects of MT leads to an escalation of pathogenic processes and carcinogenesis. 3The most extensive area is MTs expression for oncological applications, where the information about gene patterns is helpful for the identification biological function, resistance to drugs and creating the correct chemotherapy. In other medical applications the effect of oxidative stress to cell lines exposed to heavy metals and hydrogen peroxide is studied as well as influence of drugs and cytokines on MTs expression and MTs expression in the adiposetissue. The precise detection of low metallothionein concentrations and its isoforms is necessary to understand the connection between quantity and isoforms of MTs to size, localization and type of cancer. This information is necessary for well-timed therapy and increase the chance to survival. Microarray chips appear as good possibility for finding all information about expression of MTs genes and isoforms not only in cancer, but also in other diseases, especially diabetes, obesity, cardiovascular diseases, ageing, osteoporosis, psychiatric disorders and as the effects of toxic drugs and pollutants, which is discussed in this review. List of abbreviations:HCC -hepatocellular carcinoma, HFD -high fat diet, MMP -matrix metalloproteinase, MT -metallothionein, MTF -metal transcription factor, MRE -metal responsive element, ROS -reactive oxygen species.

show abstract

Section: Cardiovascular Diseasesmentioning

confidence: 99%

Microarray analysis of metallothioneins in human diseases—A review

Křížková

Kępińska

Emri

et al. 2016

Journal of Pharmaceutical and Biomedical Analysis

View full text Add to dashboard Cite

show abstract

“…We should note, however, that because most studies to date have been carried out in cell culture and at super-physiological concentrations, there remain high returns to research focusing on the magnitude of these benefits under more natural conditions. Another pathway associated with longevity extension that is activated by xenohormetic compounds involves transcription factor nuclear factor-E2-related factor 2 (Nrf2; Godman et al 2009;Onken and Driscoll 2010). Nrf2 binds antioxidant response elements upstream of cytoprotective stress response proteins such as heme oxygenase and Hsp 22/40/90.…”

Section: Xenohormetic Compounds and Longevity Pathwaysmentioning

confidence: 99%

Xenohormesis: health benefits from an eon of plant stress response evolution

Hooper

Hooper²,

Tytell

et al. 2010

Cell Stress and Chaperones

View full text Add to dashboard Cite

Xenohormesis is a biological principle that explains how environmentally stressed plants produce bioactive compounds that can confer stress resistance and survival benefits to animals that consume them. Animals can piggyback off products of plants' sophisticated stress response which has evolved as a result of their stationary lifestyle. Factors eliciting the plant stress response can judiciously be employed to maximize yield of health-promoting plant compounds. The xenohormetic plant compounds can, when ingested, improve longevity and fitness by activating the animal's cellular stress response and can be applied in drug discovery, drug production, and nutritional enhancement of diet.

show abstract

“…(d) As shown in Fig. 2 [21]. Hence, in this study, a widely used endothelial cell line HUVECs was adopted to explore ROS origination.…”

Section: Discussionmentioning

confidence: 99%

A Novel Approach to Estimate ROS Origination by Hyperbaric Oxygen Exposure, Targeted Probes and Specific Inhibitors

Zhou¹,

Huang²,

Yu³

et al. 2018

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: Reactive oxygen species (ROS) are considered fundamental in various physiological/pathophysiological processes and prevention/treatment measures such as hyperbaric oxygen (HBO) therapy. In this study, the origination of ROS in human umbilical vein endothelial cells was investigated under basal and HBO conditions. Methods: Whole cell or mitochondria-targeted fluorescent probes were applied to mark superoxide anion (O₂^-), and the ROS produced from mitochondrial respiratory chain (MRC), NADPH oxidase (NOX) and xanthine oxidase (XO) were identified by flow cytometry, confocal imaging and microplate fluorometry with or without specific inhibitors. An algorithm was established to calculate ROS proportion of each source. Results: HBO increased ROS to about 2.14-2.44 fold in mitochondria and 1.32-1.42 fold in whole cell. Then ROS levels were significantly decreased by MRC inhibition about 30% and 16%, respectively. NOX or XO inhibition did not affect HBO-induced ROS generation. Based on these data, it could be further estimated that mitochondrial ROS accounted for 32%-39% of basal whole-cell ROS including 3% from MRC complex II, and NOX accounted for at least 24%-29%. Following HBO treatment, almost all increased ROS originated from mitochondria, and MRC complex II contributed at least 45%-60%. Conclusion: This study provided a simple but effective method to estimate the origination of intracellular ROS and found that MRC were the main source of HBO-induced ROS generation.

show abstract

Hyperbaric oxygen induces a cytoprotective and angiogenic response in human microvascular endothelial cells

Cited by 122 publications

References 34 publications

Microarray analysis of metallothioneins in human diseases—A review

Microarray analysis of metallothioneins in human diseases—A review

Xenohormesis: health benefits from an eon of plant stress response evolution

A Novel Approach to Estimate ROS Origination by Hyperbaric Oxygen Exposure, Targeted Probes and Specific Inhibitors

Contact Info

Product

Resources

About