Hyperbaric oxygen therapy given 30 minutes after spinal cord ischemia attenuates selective motor neuron death in rabbits

Murakami, Noritaka; Horinouchi, Takashi; Sakurai, Masahiro; Ejima, Yutaka; Matsukawa, Shu; Kato, Masato; Tabayashi, Koichi

doi:10.1097/00003246-200104000-00027

Cited by 35 publications

(22 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Thus, the balloon at the distal end of the catheter was positioned 0.5-1.5 cm distally to the origin of the left renal artery. Other study 8 and preliminary investigations by injecting radiocontrast dye into the abdominal aorta via the distal hole of the catheter or by laparotomy confirmed that the balloon was positioned 0.5-1.5 cm distal to the left renal artery (Figure 1). PaO 2 , PaCO 2 and Hb were performed and pH was measured at baseline, during ischemia, and 10 min of reperfusion following ischemia.…”

Section: Animal Modelmentioning

confidence: 90%

The effect of thalidomide on spinal cord ischemia/reperfusion injury in a rabbit model

et al. 2006

View full text Add to dashboard Cite

Study design: Randomized study. Objectives: To evaluate the effects of thalidomide on spinal cord ischemia/reperfusion injury via reduced TNF-a production. Setting: Animal experimental laboratory, Clinical Research Institute of Seoul National University Hospital, Seoul, Korea. Methods: Spinal cord ischemia was induced in rabbits by occluding the infrarenal aorta. Rabbits in group N did not undergo ischemic insult, but rabbits in groups C (the untreated group), THA, and THB underwent ischemic insult for 15 min. The THA and THB groups received thalidomide (20 mg/kg) intraperitoneally (i.p.) before ischemia, but only the THB group received thalidomide (i.p., 20 mg/kg) after 24 and 48 h of reperfusion. After evaluating neurologic functions at 1.5 h, 3, and 5 days of reperfusion, rabbits were killed for histopathologic examination and Western blot analysis of TNF-a. Results: The THA and THB groups showed significantly less neurologic dysfunction than the C group at 1.5 h, 3, and 5 days of reperfusion. The number of normal spinal motor neurons in ventral gray matter was higher in THA and THB than in C, but no difference was observed between THA and THB. Western blot analysis showed a significantly higher level of TNF-a in C than in THA and THB at 1.5 h of reperfusion, but no difference was observed between C, THA, or THB at 3 or 5 days of reperfusion. Conclusion: Thalidomide treatment before ischemic insult reduces early phase ischemia/ reperfusion injury of the spinal cord in rabbits.

show abstract

Section: Animal Modelmentioning

confidence: 90%

The effect of thalidomide on spinal cord ischemia/reperfusion injury in a rabbit model

et al. 2006

View full text Add to dashboard Cite

show abstract

“…In experiments with animal models, various application times of the HBOT were used, from 15 min after the lesion, 12 to 2 18 to 4 h. 19 In humans, it is recommended 20 that hyperbaric therapy be initiated within 4 h of the spinal cord lesion. In this study, the start of therapy in the hyperbaric chamber occurred 24 h after the spinal cord model lesion was produced in the animals, and it is possible that the effects of HBOT would be more intense if administrated earlier, a possibility that should be investigated in future studies.…”

Section: Discussionmentioning

confidence: 99%

“…In this study, the start of therapy in the hyperbaric chamber occurred 24 h after the spinal cord model lesion was produced in the animals, and it is possible that the effects of HBOT would be more intense if administrated earlier, a possibility that should be investigated in future studies. Other studies 12,18,19,21 use oxygen under pressure at twofive ATA in animals submitted to induced spinal cord lesion. Above these pressures, the side effects, such as necrosis of the cells of the anterior horn of the spinal cord in rats, or enzyme degradation by oxidation in the brain, may occur.…”

Section: Discussionmentioning

confidence: 99%

“…11,12 A tubular hyperbaric chamber was used, measuring 770 mm in length and 180 mm in internal diameter, with a useful height of 150 mm (between the platform and the upper wall), transparent, acrylic walls of 10 mm in thickness and capacity to hold 10 rats simultaneously. The hyperbaric chamber was regulated to provide 14 l of oxygen per minute.…”

Section: 16mentioning

confidence: 99%

“…9 However, its mortality rate is high. 10 Another physical procedure that can be used is hyperbaric oxygen therapy (HBOT), 11,12 in which high partial pressures of tissue oxygen are obtained, which are higher than the atmospheric pressure. HBOT is based on the premise that a decrease in perfusion can be compensated for by an increase in partial oxygen pressure.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Potentializing the effects of GM1 by hyperbaric oxygen therapy in acute experimental spinal cord lesion in rats

et al. 2010

View full text Add to dashboard Cite

Study design: Experimental, controlled, animal study. Objectives: To evaluate the effect of GM1 ganglioside, hyperbaric oxygen and both in combination, in the treatment of experimental spinal cord lesions in rats. Setting: Brazil. Methods: Thirty-two Wistar rats with spinal cord lesions were divided into four groups: one group received GM1 ganglioside, one was submitted to hyperbaric oxygen therapy (HBOT), the third received both treatments and the fourth received no treatment (control). Results: There were no significant differences between the groups in the histological analysis, for any of the variables (necrosis, hemorrhage, hyperemia, cystic degeneration, P40.06). Neither were there any significant differences in the comparison of left and right sides in the functional tests (P40.06 for all). No significant differences were found in the locomotor ratings, in the comparison of groups at 2, 7, 21 and 28 days after the surgical procedure. However, in the evaluation on day 14, group 3, which received the combined therapy, showed a significantly higher Basso Beattie and Bresnahan score than the other groups (P ¼ 0.015). Conclusion: The therapeutic effect of GM1 in locomotor evaluation of rats submitted to spinal cord lesion is anticipated by HBOT.

show abstract

Brain Injury and Spinal Cord Injury

Schmutz

Handbook on Hyperbaric Medicine

View full text Add to dashboard Cite

Hyperbaric oxygen therapy given 30 minutes after spinal cord ischemia attenuates selective motor neuron death in rabbits

Abstract: HBO therapy shortly after ischemic insult had protective effects against ischemic spinal cord damage. However, delayed treatment with HBO did not change the prognosis.

Cited by 35 publications

References 32 publications

The effect of thalidomide on spinal cord ischemia/reperfusion injury in a rabbit model

The effect of thalidomide on spinal cord ischemia/reperfusion injury in a rabbit model

Potentializing the effects of GM1 by hyperbaric oxygen therapy in acute experimental spinal cord lesion in rats

Brain Injury and Spinal Cord Injury

Contact Info

Product

Resources

About