Hyperbaric Oxygen Treatment Produces an Antinociceptive Response Phase and Inhibits Astrocyte Activation and Inflammatory Response in a Rat Model of Neuropathic Pain

Abstract

Hyperbaric oxygen (HBO) treatment has been proven to be a promising candidate for protection of the nervous system after acute injury in animal models of neuropathic pain. The purposes of this study were to examine the antinociceptive response phase induced by HBO treatment in a model of neuropathic pain and to determine the dependence of the treatment's mechanism of alleviating neuropathic pain on the inhibition of spinal astrocyte activation. Neuropathic pain was induced in rats by chronic constriction injury of the sciatic nerve. Mechanical threshold and thermal latency were tested preoperatively and for 1 week postoperatively, four times daily at fixed time points. Methane dicarboxylic aldehyde (MDA) and superoxide dismutase (SOD) parameters were used as indices of oxidative stress response and tested before and after the treatment. The inflammatory cytokines interleukin (IL)-1β and IL-10 were assayed in the sciatic nerve were with enzyme-linked immunoassay. Glial fibrillary acidic protein activation in the spinal cord was evaluated immunohistochemically. The rats exhibited temporary allodynia immediately after HBO treatment completion. This transient allodynia was closely associated with changes in MDA and SOD levels. A single HBO treatment caused a short-acting antinociceptive response phase. Repetitive HBO treatment led to a long-acting antinociceptive response phase and inhibited astrocyte activation. These results indicated that HBO treatment played a dual role in the aggravation and alleviation of neuropathic pain, though the aggravated pain effect (transient allodynia) was far less pronounced than the antinociceptive phase. Astrocyte inhibition and anti-inflammation may contribute to the antinociceptive effect of HBO treatment after nerve injury.

Kindlin-1 Regulates Astrocyte Activation and Pain Sensitivity in Rats With Neuropathic Pain

“…6 Our previous study demonstrated the involvement of activated astrocytes in the generation of hyperalgesia. 7 Blocking the secretion of the proinflammatory chemokines or balancing the production of proinflammatory and anti-inflammatory cytokines has been found to attenuate pain hypersensitivity. 8 In light of these findings, the inflammatory response modulated by activated astrocytes appears to be an important event in the pathogenesis of neuropathic pain.…”

Hyperbaric Oxygen Pretreatment Improves Cognition and Reduces Hippocampal Damage Via p38 Mitogen-Activated Protein Kinase in a Rat Model

“…HBO therapy was conducted as previously described 13. The chamber was filled with pure oxygen for 10 min.…”

“…13 Sections were probed with rabbit polyclonal anti-glial fibrillary acidic protein (anti-GFAP) primary antibody (1:1000 dilution; Dako, Copenhagen, Denmark). On the second day, the samples were incubated with goat anti-rabbit biotinylated secondary antibody (1:500 dilution; Beijing Zhongshan Golden Bridge Biological Technology Co. Ltd., China), followed by incubation in SABC complex (Vector Laboratories, CA, USA).…”

The bactericidal effect of 470-nm light and hyperbaric oxygen on methicillin-resistant Staphylococcus aureus (MRSA)

“…Moreover, it inhibits astrocyte activation and inflammatory response [25]. HBO therapy involves the administration of 100% of oxygen at a pressure higher than 1 atmosphere (atm) at sea level [26] for times not shorter than 60 minutes as delineated by the European Consensus Conference on Hyperbaric Medicine held in Lille, France in 1994 [27].…”