Hypercaloric Diet Induces Astrogliosis in Several Areas of the Rat Brain

Bondan, Eduardo Fernandes; Molina, Jéssica; Dossa, P.D.; Viebig, L.B.; Bonamin, Leoni Villano; Martins, M.F.; Joaquim, A.; Kirsten, Thiago Berti; Cardoso, Cristiane de Almeida Baldini; Bernardi, Maria Martha

doi:10.1016/j.jcpa.2017.10.111

Cited by 2 publications

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“…Neuroinflammation in the CNS has been increasingly identified as a feature of obesity in animals [9][10][11][12][13]18,19,20 , not only in the hypothalamus, where it was first recognized, but also in other brain areas 13 . It is widely recognized that obesogenic diets induce early inflammatory effects in ND group: rats fed with normocaloric (standard control) diet; HD group: rats fed with hypercaloric (cafeteria) diet.…”

Section: Discussionmentioning

confidence: 99%

“…Because of their physical proximity to blood vessels, and their function in transporting nutrients, astrocytes are directly affected by nutrient excess, leading to hypothalamic astrogliosis 12 . Later, it was shown that other CNS areas presented the same type of astrocyte response, such as the parietal and frontal cortex, the corpus callosum, the nucleus accumbens, the pons and the molecular and granular layers of the cerebellum 13 .…”

Section: Introductionmentioning

confidence: 99%

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Memory impairments and increased GFAP expression in hippocampal astrocytes following hypercaloric diet in rats

Bondan

Cardoso

Martins

et al. 2019

Arq. Neuro-Psiquiatr.

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Objective: Hypothalamic inflammation and glial fibrillary acidic protein (GFAP) overexpression in astrocytes are well described in obese animals, as are some cognitive and memory deficits. As the hippocampus plays important roles in the consolidation of information, this investigation aimed to observe the memory function and the astrocyte expression of GFAP in the hippocampus of rats that received either a hypercaloric or a normocaloric diet. Methods: Adult male Wistar rats received a high-fat (cafeteria) or a standard diet for 60 days. On the 61st day, the rats were submitted to the novel object recognition (NOR) test at three and 24 hours after the first contact with objects, to assess short-term and long-term memory, respectively. Thereafter, the rats were euthanized and their brains were collected for GFAP immunohistochemical investigation in the hippocampus (CA1, CA2, CA3 areas) and hypothalamus (periventricular and arcuate nuclei). Astrocytic reactivity was assessed by morphometry. Different white adipose tissue depots and brown adipose tissue were weighed to calculate the adiposity index. Results: The hypercaloric diet increased body weight gain, adiposity index, white adipose tissue weight (epididymal, subcutaneous and retroperitoneal) and brown adipose tissue weight. Rats fed with the hypercaloric diet showed short-term and long-term memory impairments in the NOR test, as well as increased GFAP expression in astrocytes from all analyzed hypothalamic and hippocampal areas. Conclusion: This astrogliosis suggests that the neuroinflammatory response also occurs in the hippocampus and may be involved in the memory losses observed in obese/overweight animals.

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