Hyperendemic Dengue and Possible Zika Circulation in the Westernmost Region of the Indonesian Archipelago

Harapan, Harapan; Panta, Kritu; Michie, Alice; Ernst, Timo; McCarthy, Suzi; Muhsin, Muhsin; Safarianti, Safarianti; Zanaria, Tjut Mariam; Mudatsir, Mudatsir; Sasmono, R. Tedjo; Imrie, Allison

doi:10.3390/v14020219

Cited by 4 publications

(4 citation statements)

References 63 publications

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“…Despite a previous study reporting different cross-neutralization patterns observed in various ZIKV and DENV infections, whether a defined neutralizing antibody titer (PRNT 50 titer) can discriminate sDENV and DENV + ZIKV panels or it can be applied to regions where other flaviviruses (YFV, WNV or JEV) are prevalent remains unclear [ 62 ]. A recent study in Indonesian Archipelago, a DENV hyperendemic region, revealed multitypic neutralizing antibodies to two or more DENV serotypes and suggested possible ZIKV circulation based on high stringent PRNT 90 titers, underscoring the difficulty of using PRNT to delineate DENV and ZIKV infections in hyperendemic regions [ 63 ]. Other studies used two-step neutralization tests (initial screening by PRNT to ZIKV followed by PRNT to DENV1-4 and ZIKV) [ 51 , 52 ] or combination of IgG ELISA and neutralization test (for positive or equivocal samples) to investigate ZIKV or DENV seroprevalence in endemic regions [ 64-66 ].…”

Section: Discussionmentioning

confidence: 99%

Detection of anti-premembrane antibody as a specific marker of four flavivirus serocomplexes and its application to serosurveillance in endemic regions

Chen,

Dai,

Hsieh

et al. 2024

Emerging Microbes & Infections

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In the past few decades, several emerging/re-emerging mosquito-borne flaviviruses have resulted in disease outbreaks of public health concern in the tropics and subtropics. Due to cross-reactivities of antibodies recognizing the envelope protein of different flaviviruses, serosurveillance remains a challenge. Previously we reported that anti-premembrane (prM) antibody can discriminate between three flavivirus infections by Western blot analysis. In this study, we aimed to develop a serological assay that can discriminate infection or exposure with flaviviruses from four serocomplexes, including dengue (DENV), Zika (ZIKV), West Nile (WNV) and yellow fever (YFV) viruses, and explore its application for serosurveillance in flavivirus-endemic countries. We employed Western blot analysis including antigens of six flaviviruses (DENV1, 2 and 4, WNV, ZIKV and YFV) from four serocomplexes. We tested serum samples from YF-17D vaccinees, and from DENV, ZIKV and WNV panels that had been confirmed by RT–PCR or by neutralization assays. The overall sensitivity/specificity of anti-prM antibodies for DENV, ZIKV, WNV, and YFV infections/exposure were 91.7%/96.4%, 91.7%/99.2%, 88.9%/98.3%, and 91.3%/92.5%, respectively. When testing 48 samples from Brazil, we identified multiple flavivirus infections/exposure including DENV and ZIKV, DENV and YFV, and DENV, ZIKV and YFV. When testing 50 samples from the Philippines, we detected DENV, ZIKV, and DENV and ZIKV infections with a ZIKV seroprevalence rate of 10%, which was consistent with reports of low-level circulation of ZIKV in Asia. Together, these findings suggest that anti-prM antibody is a flavivirus serocomplex-specific marker and can be employed to delineate four flavivirus infections/exposure in regions where multiple flaviviruses co-circulate.

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Section: Discussionmentioning

confidence: 99%

Detection of anti-premembrane antibody as a specific marker of four flavivirus serocomplexes and its application to serosurveillance in endemic regions

Chen,

Dai,

Hsieh

et al. 2024

Emerging Microbes & Infections

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show abstract

“…Despite a previous study reporting different cross-neutralization patterns observed in various ZIKV and DENV infections, whether a defined neutralizing antibody titer (PRNT50 titer) can discriminate sDENV and DENV+ZIKV panels or it can be applied to regions where other flaviviruses (YFV, WNV or JEV) are prevalent remains unclear [60]. A recent study in Indonesian Archipelago, a DENV hyperendemic region, revealed multitypic neutralizing antibodies to two or more DENV serotypes and suggested possible ZIKV circulation based on high stringent PRNT90 titers, underscoring the difficulty of using PRNT to delineate DENV and ZIKV infections in hyperendemic regions [61]. Other studies used two-step neutralization tests (initial screening by PRNT to ZIKV followed by PRNT to DENV1-4 and ZIKV) [48,49] or combination of IgG ELISA and neutralization test (for positive or equivocal samples) to investigate ZIKV or DENV seroprevalence in endemic regions [62][63][64].…”

Section: Discussionmentioning

confidence: 99%

Detection of anti-premembrane antibody as a specific marker of four flavivirus serocomplexes and its application to serosurveillance in endemic regions

Chen,

Dai,

Hsieh

et al. 2023

Preprint

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In the past few decades, several emerging/re-emerging mosquito-borne flaviviruses have resulted in disease outbreaks of public health concern in the tropics and subtropics. Due to cross-reactivities of antibodies recognizing the envelope protein of different flaviviruses, serosurveillance remains a challenge. Previously we reported that anti-premembrane (prM) antibody can discriminate between three flavivirus infections by Western blot analysis. In this study, we aimed to develop a serological assay that can discriminate infection or exposure with flaviviruses from four serocomplexes, including dengue (DENV), Zika (ZIKV), West Nile (WNV) and yellow fever (YFV) viruses, and explore its application for serosurveillance in flavivirus-endemic countries. We employed Western blot analysis including antigens of six flaviviruses (DENV1, 2 and 4, WNV, ZIKV and YFV) from four serocomplexes. We tested serum samples from YF-17D vaccinees, and from DENV, ZIKV and WNV panels that had been confirmed by RT-PCR or by neutralization assays. The overall sensitivity/specificity of anti-prM antibodies for DENV, ZIKV, WNV, and YFV infections/exposure were 91.7%/96.4%, 91.7%/99.2%, 88.9%/98.3%, and 91.3%/92.5%, respectively. When testing 48 samples from Brazil, we identified multiple flavivirus infections/exposure including DENV and ZIKV, DENV and YFV, and DENV, ZIKV and YFV. When testing 50 samples from the Philippines, we detected DENV, ZIKV, and DENV and ZIKV infections with a ZIKV seroprevalence rate of 10%, which was consistent with reports of low-level circulation of ZIKV in Asia. Together, these findings suggest that anti-prM antibody is a flavivirus serocomplex-specific marker and can be employed to delineate four flavivirus infections/exposure in regions where multiple flaviviruses co-circulate.

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“…Zika virus infection has historically been a neglected viral infection in Africa, the Americas, Asia, and the Pacific, likely due to the prevalence of silent infections and relatively mild clinical manifestations 26 , 27 . Southeast Asian countries such as Thailand 28 , Cambodia 29 , 30 , Indonesia 31 , the Philippines 32 , India 33 , Singapore 34 , Japan 35 , and Vietnam 36 , 37 have experienced continuous circulation of ZIKV. Thailand, a geographically diverse country and popular tourist destination, has seen cases of ZIKV infection among travelers to various regions, including Japan 38 , Europe 39 , Israel 40 , and Canada 41 .…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity and phylogenetic analyses of Asian lineage Zika virus whole genome sequences derived from Culex quinquefasciatus mosquitoes and urine of patients during the 2020 epidemic in Thailand

Phumee,

Chitcharoen,

Sutthanont

et al. 2023

Sci Rep

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Zika virus (ZIKV), a mosquito-borne flavivirus, has been continually emerging and re-emerging since 2010, with sporadic cases reported annually in Thailand, peaking at over 1000 confirmed positive cases in 2016. Leveraging high-throughput sequencing technologies, specifically whole genome sequencing (WGS), has facilitated rapid pathogen genome sequencing. In this study, we used multiplex amplicon sequencing on the Illumina Miseq instrument to describe ZIKV WGS. Six ZIKV WGS were derived from three samples of field-caught Culex quinquefasciatus mosquitoes (two males and one female) and three urine samples collected from patients in three different provinces of Thailand. Additionally, successful isolation of a ZIKV isolate occurred from a female Cx. quinquefasciatus. The WGS analysis revealed a correlation between the 2020 outbreak and the acquisition of five amino acid changes in the Asian lineage ZIKV strains from Thailand (2006), Cambodia (2010 and 2019), and the Philippines (2012). These changes, including C-T106A, prM-V1A, E-V473M, NS1-A188V, and NS5-M872V, were identified in all seven WGS, previously linked to significantly higher mortality rates. Furthermore, phylogenetic analysis indicated that the seven ZIKV sequences belonged to the Asian lineage. Notably, the genomic region of the E gene showed the highest nucleotide diversity (0.7–1.3%). This data holds significance in informing the development of molecular tools that enhance our understanding of virus patterns and evolution. Moreover, it may identify targets for improved methods to prevent and control future ZIKV outbreaks.

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Hyperendemic Dengue and Possible Zika Circulation in the Westernmost Region of the Indonesian Archipelago

Cited by 4 publications

References 63 publications

Detection of anti-premembrane antibody as a specific marker of four flavivirus serocomplexes and its application to serosurveillance in endemic regions

Detection of anti-premembrane antibody as a specific marker of four flavivirus serocomplexes and its application to serosurveillance in endemic regions

Detection of anti-premembrane antibody as a specific marker of four flavivirus serocomplexes and its application to serosurveillance in endemic regions

Genetic diversity and phylogenetic analyses of Asian lineage Zika virus whole genome sequences derived from Culex quinquefasciatus mosquitoes and urine of patients during the 2020 epidemic in Thailand

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