We perform laser spectroscopy of Yb + ions on the 4f 14 6s 2 S 1/2 -4f 13 5d6s 3 D[3/2] 1/2 transition at 297 nm. The frequency measurements for 170 Yb + , 172 Yb + , 174 Yb + , and 176 Yb + reveal the specific mass shift as well as the field shifts. In addition, we demonstrate laser cooling of Yb + ions using this transition and show that light at 297 nm can be used as the second step in the photoionization of neutral Yb atoms.