Hyperglycemia induces PAI-1 gene expression in adipose tissue by activation of the hexosamine biosynthetic pathway

Gabriely, Ilan; Yang, Xiao Man; Cases, Jane A.; Xi, Hui; Rossetti, Luciano; Barzilai, Nir

doi:10.1016/s0021-9150(01)00574-3

Cited by 60 publications

(32 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This finding is in agreement with the study by Bouchard [18], but not with the findings of Alessi [8]. The differences between patients with and without DM could not be confirmed in our study due the small sample size, although it has been reported that hyperglycemia can lead to the increased expression of PAI-1 in adipose tissue [19]. We might also speculate about the participation of other regulatory pathways in the response of the peritoneal membrane tissues to PD.…”

Section: Fibrinolytic System Components In Peritoneal Membrane and Thsupporting

confidence: 79%

Adipocytes derived fibrinolytic components in peritoneum — a pilot study

Opatrná

Korabečná²,

Křížková

et al. 2012

Open Medicine

View full text Add to dashboard Cite

AbstractThe proteins of the fibrinolytic system — urokinase plasminogen activator(uPA), tissue plasminogen activator (tPA)and plasminogen activator inhibitor type IPAI-I) — play important roles in fibrotization in various organs and including peritoneum. To study the cellular localization of PAI-1, tPA and uPA within the adipose tissue of the peritoneal membrane in patients at the onset of peritoneal dialysis(PD) we determined the initial expression of these proteins in relationship to multiple clinical variables. Methods: routinely performed parietal peritoneal biopsies in 12 patients undergoing peritoneal catheter implantation were examined. We used formalinfixed, paraffin-embedded specimens for immunohistochemical localization of these proteins along with the stereological pointcounting method for quantification of their expression within the peritoneal adipose tissue. Results: strong positive mutual correlation between the expression of PAI-1 and both uPA (SpearmanR=0.66) and tPA (R=0.59) as well as between the expression of uPA and tPA (R=0.77) was found without any relatioship to BMI, age, peritoneal transport characteristic or diabetes status. Conclusion: Adipose tissue within the peritoneum is capable of producing fibrinolysis regulators (independently on clinical parameters) thus possibly affecting the fibrotization and function of peritoneum as dialysis membrane. The effect of dialysis solution dosing, composition and other dialysis related factors should be clarified in future studies.

show abstract

Section: Fibrinolytic System Components In Peritoneal Membrane and Thsupporting

confidence: 79%

Adipocytes derived fibrinolytic components in peritoneum — a pilot study

Opatrná

Korabečná²,

Křížková

et al. 2012

Open Medicine

View full text Add to dashboard Cite

show abstract

“…In particular, reduction of intra-abdominal ("visceral") adipose tissue by surgical removal (24) or as a result of CR (12) is associated with a significant improvement in glucose tolerance in mice. Differences in distribution of adipose tissue could, in principle, influence circulating levels of adipocyte-derived cytokines (adipokines) such as adiponectin and resistin (45), which modulate insulin sensitivity.…”

Section: Discussionmentioning

confidence: 99%

Long-lived crowded-litter mice exhibit lasting effects on insulin sensitivity and energy homeostasis

Sadagurski

Landeryou

Blandino-Rosano

et al. 2014

American Journal of Physiology-Endocrinology and Metabolism

View full text Add to dashboard Cite

The action of nutrients on early postnatal growth can influence mammalian aging and longevity. Recent work has demonstrated that limiting nutrient availability in the first 3 wk of life [by increasing the number of pups in the crowdedlitter (CL) model] leads to extension of mean and maximal lifespan in genetically normal mice. In this study, we aimed to characterize the impact of early-life nutrient intervention on glucose metabolism and energy homeostasis in CL mice. In our study, we used mice from litters supplemented to 12 or 15 pups and compared those to control litters limited to eight pups. At weaning and then throughout adult life, CL mice are significantly leaner and consume more oxygen relative to control mice. At 6 mo of age, CL mice had low fasting leptin concentrations, and low-dose leptin injections reduced body weight and food intake more in CL female mice than in controls. At 22 mo, CL female mice also have smaller adipocytes compared with controls. Glucose and insulin tolerance tests show an increase in insulin sensitivity in 6 mo old CL male mice, and females become more insulin sensitive later in life. Furthermore, ␤-cell mass was significantly reduced in the CL male mice and was associated with reduction in ␤-cell proliferation rate in these mice. Together, these data show that early-life nutrient intervention has a significant lifelong effect on metabolic characteristics that may contribute to the increased lifespan of CL mice.

show abstract

“…The molecular pathways that mediate the effects of fatty acids and glucose on gene transcription have been partially characterised [16][17][18]. Several of the genes investigated in this study are directly regulated by fatty acids (adipocyte lipid binding protein, uncoupling protein 2 and angiotensinogen) and glucose (leptin, plasminogen activator inhibitor 1, fatty acid synthase and hormone-sensitive lipase) in adipocytes [25][26][27][28][29][30][31]. The greater reduction in plasma NEFA levels in the low-fat group compared with the high-fat group had no impact on gene expression.…”

Section: Discussionmentioning

confidence: 99%

Adipose tissue gene expression in obese subjects during low-fat and high-fat hypocaloric diets

et al. 2004

View full text Add to dashboard Cite

Aims/hypothesis: Adaptation to energy restriction is associated with changes in gene expression in adipose tissue. However, it is unknown to what extent these changes are dependent on the energy restriction as such or on the macronutrient composition of the diet. Methods: We determined the levels of transcripts for 38 genes that are expressed in adipose tissue and encode transcription factors, enzymes, transporters and receptors known to play critical roles in the regulation of adipogenesis, mitochondrial respiration, and lipid and carbohydrate metabolism. Two groups of 25 obese subjects following 10-week hypocaloric diet programmes with either 20-25 or 40-45% of total energy derived from fat were investigated. Levels of mRNA were measured by performing real-time RT-PCR on subcutaneous fat samples obtained from the subjects before and after the diets. Results: The two groups of subjects lost 7 kg over the duration of the diets. Ten genes were regulated by energy restriction; however, none of the genes showed a significantly different response to the diets. Levels of peroxisome proliferator-activated receptor γ co-activator 1α mRNA were increased, while the expression of the genes encoding leptin, osteonectin, phosphodiesterase 3B, hormone-sensitive lipase, receptor A for natriuretic peptide, fatty acid translocase, lipoprotein lipase, uncoupling protein 2 and peroxisome proliferator-activated receptor γ was

show abstract

Hyperglycemia induces PAI-1 gene expression in adipose tissue by activation of the hexosamine biosynthetic pathway

Cited by 60 publications

References 49 publications

Adipocytes derived fibrinolytic components in peritoneum — a pilot study

Adipocytes derived fibrinolytic components in peritoneum — a pilot study

Long-lived crowded-litter mice exhibit lasting effects on insulin sensitivity and energy homeostasis

Adipose tissue gene expression in obese subjects during low-fat and high-fat hypocaloric diets

Contact Info

Product

Resources

About