Hyperimmunised bovine milk and whey: influence of <scp>pH</scp> and enzymatic treatments on the antigen‐binding capacity of immunoglobulin G

Menéndez, Julia Dugnol; Riera, Francisco A.

doi:10.1002/jsfa.7291

Cited by 2 publications

(1 citation statement)

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“…Hemp extract has been shown to inhibit the proliferation and growth of prostate cancer (De Petrocellis et al., 2013) and melanoma (Blázquez et al., 2006)cells, and hemp derivatives are able to alter mitochondrial permeability of rat liver cells and increase cytochrome c release (Zaccagnino et al., 2012), suggesting that hemp peptides represent candidate anticancer agents. It has also been reported that the biological activities of peptides are modulated by molecular weight, with low molecular weight correlating with increased activity (Dugnol & Riera, 2016; Li et al., 2019).…”

Section: Discussionmentioning

confidence: 99%

Anticancer property of Hemp Bioactive Peptides in Hep3B liver cancer cells through Akt/GSK3β/β‐catenin signaling pathway

Wei

Dong

Sun

et al. 2021

Food Science & Nutrition

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Foodborne protein hydrolysates exhibit biological activity that may be therapeutic in a number of human disease settings. Hemp peptides (HP) generated by controlled hydrolysis of hemp proteins have a number of health benefits and are of pharmaceutical value. In the present study, we produce small molecular weight HP from hemp seed and investigate its anticancer properties in Hep3B human liver cancer cells. We demonstrate that HP treatment increased apoptosis, reduced cell viability, and reduced cell migration in Hep3B human liver cancer cells without affecting the normal liver cell line L02. We correlate these phenotypes with increased cellular ROS levels, upregulation of cleaved caspase 3 and Bad, and downregulation of antiapoptotic Bcl‐2. HP treatment led to increased Akt and GSK‐3β phosphorylation, with subsequent downregulation of β‐catenin, suggesting β‐catenin signaling modulation as a critical mechanism by which HP exhibits anticancer properties. Our findings suggest HP are of potential therapeutic interest for liver cancer treatment.

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