Hyperin promotes proliferation, migration, and invasion of HTR-8/SVneo trophoblast cells via activation of JAK1/STAT3 pathway in recurrent spontaneous abortions

Mu, Zhenni; Shen, Sinan; Tang, Li; Liu, Yingdie; Zhou, Ziwei; Lei, Lei

doi:10.1016/j.heliyon.2023.e12958

Cited by 6 publications

(2 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A balance must be maintained between pro-and anti-in ammatory activity within the placenta during pregnancy. Disruption of this balance is associated with abnormalities in the development and function of the placental vasculature, which can lead to recurrent spontaneous abortion (RSA) and other complications (47). It has been found that adverse pregnancy outcomes in miscarriage-prone mice are associated with decreased placental hemodynamics caused by placental in ammation(48).…”

Section: Discussionmentioning

confidence: 99%

Bioinformatics study and experimental validation of METTL3 regulation of immune-related genes affecting placental vascular development

Zhao,

Du,

Yang

et al. 2023

Preprint

View full text Add to dashboard Cite

The proper development of the placental vascular system is a crucial factor in ensuring fetal health. m6A modification is a key pathophysiological mechanism in placental vascular development. However, the specific mechanism by which m6A influences placental vascular development remains unclear. Here, we explored the role of 21 m6A regulators in placental development based on the Gene Expression Omnibus (GEO) database. Following a series of machine learning techniques, METTL3 was recognized as the pivotal m6A regulator. We subsequently employed consensus clustering analysis to delineate two distinct m6A isoforms, and investigated their correlation with immune cells. Further, through weighted gene co-expression network analysis (WGCNA) coupled with correlation analysis, we pinpointed METTL3-associated placental development genes. These genes were notably enriched in immune-related categories. Furthermore, we uncovered immune-related differentially expressed genes that were associated with differentially expressed m6A regulators. Additionally, we performed an immune infiltration analysis to gain a deeper understanding of how these genes interact with immune cells. Ultimately, to validate our findings, we carried out animal experiments. In conclusion, our study found that targeting METTL3 could affect placental vascular development, which may provide guidance for the clinical treatment of placental-like diseases.

show abstract

Section: Discussionmentioning

confidence: 99%

Bioinformatics study and experimental validation of METTL3 regulation of immune-related genes affecting placental vascular development

Zhao,

Du,

Yang

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Human chorionic trophoblast cells (HTR‐8/SVneo) were obtained from ATCC (American Type Culture Collection, Manassas, VA, USA) and cultured in Roswell Park Memorial Institute (RPMI)‐1640 media (BasalMedia, Shanghai, China) with 10% fetal bovine serum (#A5669401, Thermo Fisher Scientific, USA) and 1% penicillin–streptomycin at 37°C with saturated humidity, 5% CO 2 . Cell proliferation assay was performed as a previous study (Mu et al., 2023 ). Briefly, cells at the logarithmic growth phase were collected for experiments.…”

Section: Methodsmentioning

confidence: 99%

Role of increased IGFBP2 in trophoblast cell proliferation and recurrent spontaneous abortion development: A pilot study

Ji,

Jiao,

Zhang

et al. 2024

Physiological Reports

View full text Add to dashboard Cite

Recurrent spontaneous abortion (RSA) is a serious condition that adversely affects women's health. Differentially expressed proteins (DEPs) in plasma of patients experiencing RSA is helpful to find new therapeutic targets and identified with mass spectrometry. In 57 DEPs, 21 were upregulated and 36 were downregulated in RSA. Gene ontology analyses indicated that identified DEPs were associated with cell proliferation, including significantly downregulated insulin‐like growth factor binding protein 2 (IGFBP2). Immunohistochemical result using clinical decidual tissues also showed that IGFBP2 expression was significantly decreased in RSA trophoblasts. Cell proliferation assay indicated that IGFBP2 treatment increased the proliferation and mRNA expressions of PCNA and Ki67 in trophoblast cells. Transcriptome sequencing experiments and Kyoto Encyclopedia of Genes and Genomes analyses revealed that gene expression for components in PI3K‐Akt pathway in trophoblasts was significantly upregulated following IGFBP2 treatment. To confirm bioinformatics findings, we did cell‐based experiments and found that treatment of inhibitors for insulin‐like growth factor (IGF)‐1 receptor‐PI3K‐Akt pathway significantly reduced IGFBP2‐induced trophoblast cell proliferation and mRNA expressions of PCNA and Ki67. Our findings suggest that IGFBP2 may increase trophoblast proliferation through the PI3K‐Akt signaling pathway to affect pregnancy outcomes and that IGFBP2 may be a new target for future research and treatment of RSA.

show abstract

Exploring the mechanism of Semen Cuscutae processed with salt solution in improving kidney deficiency miscarriage based on serum pharmacochemistry and network pharmacology

Zhang,

Huang,

Yang

et al. 2024

Arabian Journal of Chemistry

View full text Add to dashboard Cite

Hyperin promotes proliferation, migration, and invasion of HTR-8/SVneo trophoblast cells via activation of JAK1/STAT3 pathway in recurrent spontaneous abortions

Cited by 6 publications

References 34 publications

Bioinformatics study and experimental validation of METTL3 regulation of immune-related genes affecting placental vascular development

Bioinformatics study and experimental validation of METTL3 regulation of immune-related genes affecting placental vascular development

Role of increased IGFBP2 in trophoblast cell proliferation and recurrent spontaneous abortion development: A pilot study

Exploring the mechanism of Semen Cuscutae processed with salt solution in improving kidney deficiency miscarriage based on serum pharmacochemistry and network pharmacology

Contact Info

Product

Resources

About