Hyperlipaemia intensifies the course of acute oedematous and acute necrotising pancreatitis in the rat.

Abstract

Background-Serum triglyceride concentrations higher than 10 to 20 mmol/l are probably a risk factor for developing acute pancreatitis in humans. Aims-To therefore analyse the influence of hyperlipaemia on the course of acute oedematous and acute necrotising pancreatitis in rats. Subjects-Male Wistar rats were used in all experiments. Methods-Six different groups of animals were used: two groups without pancreatitis (controls), two with acute oedematous pancreatitis, and two with acute necrotising pancreatitis. One group from each pair was treated with Triton WR 1339, which induces endogenous hyperlipaemia. Blood samples were taken from all subjects to measure triglyceride, cholesterol, amylase, and lipase. Pancreatic tissue samples were taken and the degree of pancreatic damage was judged microscopically. Results-In the control groups no significant changes occurred, either in serum enzyme activities or in histology. The hyperlipaemic subgroup of animals with acute oedematous pancreatitis developed significantly higher (p<0.001) serum amylase activities and a greater degree of histological damage (p<0.01) than the animals of the non-hyperlipaemic acute oedematous pancreatitis group. In the animals with necrotising pancreatitis, serum lipase activity and the histological degree of pancreatic damage were significantly higher in the hyperlipaemic animals than in the non-hyperlipaemic

Physiological Capacity of the Reticuloendothelial System for the Degradation of Hemoglobin Vesicles (Artificial Oxygen Carriers) after Massive Intravenous Doses by Daily Repeated Infusions for 14 Days

“…A similar tendency was observed after the bolus HbV infusion (20 ml/kg) (Sakai et al, 2004b). This level of increment was significantly smaller than the value for the Wistar rats with acute necrotizing pancreatitis that increased the lipase activity from 10 to 475 to 5430 IU/l (Hofbauer et al, 1996). One possible reason for the moderate and specific increase in lipase activity would be related to the enzyme induction in the pancreas by the presence of a large amount of lipids from the liposomes (Stuecklin-Utsch et al, 2002), because pancreatic lipase hydrolyzes not only triglyceride but also phosphatidylcholine (Rowland and Woodley, 1980).…”

Fluconazole Penetration into the Pancreas

“…Fluconazole penetration was also analyzed in experimental edematous and necrotizing pancreatitis (n ϭ 4), which was induced as reported before (15). To rats kept awake in single cages, fluconazole (0.57 mg/100 g of body weight) was given intravenously as a bolus injection 48 or 6 h after the induction of edematous or necrotizing pancreatitis, respectively, because after these time points pancreatitis is completely established.…”

Anti-hyperlipidemic Effects of Red Ginseng Acidic Polysaccharide from Korean Red Ginseng

“…20) Briefly, sixty rats were randomized into six groups of normal, control, and RGAP groups (R100, R300, R500, and R1000) in order to evaluate the endogenous anti-hyperlipidemic effect. The RGAP groups were orally administered with RGAP dissolved in 1 ml of D.W. at doses of 100, 300, 500 and 1000 mg/kg body weight for 3 d to examine short-term preventive effect.…”