2008
DOI: 10.1007/s00125-008-1034-8
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Hypermethylation of hepatic Gck promoter in ageing rats contributes to diabetogenic potential

Abstract: Aims/hypothesis Hepatic glucokinase (GCK) is a key enzyme in glucose utilisation. Downregulation of its activity is associated with insulin resistance and type 2 diabetes mellitus. However, it is unknown whether hepatic Gck expression is influenced by age and is involved in ageing-mediated diabetes, and whether the degree of methylation of the hepatic Gck promoter is correlated with the transcription of Gck. To address the question, we evaluated hepatic Gck transcription and promoter methylation in young (14 w… Show more

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Cited by 76 publications
(62 citation statements)
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“…A chronically high secretion of insulin could explain our observation of low blood glucose and high GK activity. Alternatively, high level of hypoxia inducible factor (HIF)-1␣ (41) or hypomethylation of GK gene promoter region (42,43) are potential mechanisms accounting for high GK activity. On the other hand, high activity of GK should favor a greater availability of glucose-derived substrates for acetyl-CoA production from glycolysis.…”
Section: Discussionmentioning
confidence: 99%
“…A chronically high secretion of insulin could explain our observation of low blood glucose and high GK activity. Alternatively, high level of hypoxia inducible factor (HIF)-1␣ (41) or hypomethylation of GK gene promoter region (42,43) are potential mechanisms accounting for high GK activity. On the other hand, high activity of GK should favor a greater availability of glucose-derived substrates for acetyl-CoA production from glycolysis.…”
Section: Discussionmentioning
confidence: 99%
“…Then PCR products were sequenced using a DNA sequencer (ABI PRISM 3730, Foster City, CA, USA). Methylation level was measured according to our previously reported method (Jiang et al, 2008(Jiang et al, , 2009). …”
Section: Quantification Of Methylation Level Of Mir-146a Promotermentioning
confidence: 99%
“…To quantify the degree of liver gene expression, we applied PCR array 56 Gck encodes glucokinase phosphorylates glucose as first step of glucose utilization in hepatocytes. 57 The role of glucokinase is to provide glucose 6-phosphate for the synthesis of glycogen and fatty acids. Gpd1, Hk2 and Pklr encode for glycolytic enzymes.…”
Section: Evidence For Liver Programmingmentioning
confidence: 99%