Hyperosmolarity promotes macrophage pyroptosis by driving the glycolytic reprogramming of corneal epithelial cells in dry eye disease

Han, Yu; Zhang, Yu Alex; Yuan, Kelan; Wu, Yi; Jin, Xiuming; Huang, Xiaodan

doi:10.1007/s11684-023-0986-x

Cited by 8 publications

(2 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[ 31 ] To determine the ROS scavenging effects of n-Z(Fe), PB and PBnZ in vitro, ROS production was quantified in HCECs and CECs using a DCFH-DA probe under hypertonic conditions (HOM, 500 mOsM), which was selected to induce ROS overproduction and inflammation. [ 32 ] Fig. 3 a showed the ability of n-Z(Fe), PB and PBnZ to eliminate intracellular ROS in HCECs and CECs induced by HOM.…”

Section: Resultsmentioning

confidence: 99%

Dual-ROS-scavenging and dual-lingering nanozyme-based eye drops alleviate dry eye disease

Zhang,

Zhao,

Chu

et al. 2024

J Nanobiotechnol

View full text Add to dashboard Cite

Efficiently removing excess reactive oxygen species (ROS) generated by various factors on the ocular surface is a promising strategy for preventing the development of dry eye disease (DED). The currently available eye drops for DED treatment are palliative, short-lived and frequently administered due to the short precorneal residence time. Here, we developed nanozyme-based eye drops for DED by exploiting borate-mediated dynamic covalent complexation between n-FeZIF-8 nanozymes (n-Z(Fe)) and poly(vinyl alcohol) (PVA) to overcome these problems. The resultant formulation (PBnZ), which has dual-ROS scavenging abilities and prolonged corneal retention can effectively reduce oxidative stress, thereby providing an excellent preventive effect to alleviate DED. In vitro and in vivo experiments revealed that PBnZ could eliminate excess ROS through both its multienzyme-like activity and the ROS-scavenging activity of borate bonds. The positively charged nanozyme-based eye drops displayed a longer precorneal residence time due to physical adhesion and the dynamic borate bonds between phenyboronic acid and PVA or o-diol with mucin. The in vivo results showed that eye drops could effectively alleviate DED. These dual-function PBnZ nanozyme-based eye drops can provide insights into the development of novel treatment strategies for DED and other ROS-mediated inflammatory diseases and a rationale for the application of nanomaterials in clinical settings. Graphical Abstract

show abstract

Section: Resultsmentioning

confidence: 99%

Dual-ROS-scavenging and dual-lingering nanozyme-based eye drops alleviate dry eye disease

Zhang,

Zhao,

Chu

et al. 2024

J Nanobiotechnol

View full text Add to dashboard Cite

show abstract

“…LPS-induced cellular inflammation has been reported to be a reliable model for studying DED [34][35][36]. It was further shown that topical application of LPS increased the expression of inflammatory mediators IL-1β, IL-6, IFN-γ, and TNFα in the mouse conjunctiva, as well as IL-1β and CXCL10 in the cornea [37]. Therefore, we used LPS to induce HCE-2 for further studies.…”

Section: Anti-inflammation Efficacy Of Cel Nms In Vitromentioning

confidence: 99%

ROS-responsive celastrol-nanomedicine alleviates inflammation for dry eye disease

Cui,

Zhang,

Zhang

et al. 2024

Nanotechnology

View full text Add to dashboard Cite

Dry eye disease (DED) is a major global eye disease leading to severe eye discomfort and even vision impairment. The incidence of DED has been gradually increasing with the high frequency of use of electronic devices. It has been demonstrated that celastrol (Cel) has excellent therapeutic efficacy in ocular disorders. However, the poor water solubility and short half-life of Cel limit its further therapeutic applications. In this work, a reactive oxygen species (ROS) sensitive polymeric micelle was fabricated for Cel delivery. The micelles improve the solubility of Cel, and the resulting Cel loaded micelles exhibit an enhanced intervention effect for DED. The in vitro results demonstrated that Cel-nanomedicine had a marked ROS responsive release behavior. The results of in vitro and in vivo experiments demonstrated that Cel has excellent biological activities to alleviate inflammation in DED by inhibiting TLR4 signaling activation and reducing pro-inflammatory cytokine expression. Therefore, the Cel nanomedicine can effectively eliminate ocular inflammation, promote corneal epithelial repair, and restore the number of goblet cells and tear secretion, providing a new option for the treatment of DED.

show abstract

The alterations of ocular surface metabolism and the related immunity inflammation in dry eye

Wan,

Zhang,

Zhang

et al. 2025

Advances in Ophthalmology Practice and Research

View full text Add to dashboard Cite

Hyperosmolarity promotes macrophage pyroptosis by driving the glycolytic reprogramming of corneal epithelial cells in dry eye disease

Cited by 8 publications

References 38 publications

Dual-ROS-scavenging and dual-lingering nanozyme-based eye drops alleviate dry eye disease

Dual-ROS-scavenging and dual-lingering nanozyme-based eye drops alleviate dry eye disease

ROS-responsive celastrol-nanomedicine alleviates inflammation for dry eye disease

The alterations of ocular surface metabolism and the related immunity inflammation in dry eye

Contact Info

Product

Resources

About