Hypersensitivity Pneumonitis: Experimental Production in Guinea Pigs with Antigens of &lt;i&gt;Micropolyspora faeni&lt;/i&gt;

Wilkie, B.N.; Pauli, Bendicht U.; Gygax, M

doi:10.1159/000162686

Cited by 21 publications

(23 citation statements)

References 13 publications

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“…Edwards (1974), by means of intratracheal instillation in immunised rabbits showed that both living cells of M. faeni and glycopeptide antigens extracted from the organism provoked features of both Type I11 and Type IV reactions. These reactions were also implicated by Wilkie, Pauli and Gygax (1973) in guinea-pigs challenged with ultrasonicated cells of M. faeni after previous immunisation with soluble antigens of the organism. Factors initiating the disease were transferred by passive immunisation with plasma or lymphocytes.…”

Section: Discussionmentioning

confidence: 92%

Particulate and Soluble Antigens of Micropolyspora Faeni In Experimental Allergic Alveolitis of the Mouse

Blyth¹,

Wardrop²

1977

Journal of Medical Microbiology

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H E A L T H Y farmers often possess strong precipitating antibodies to Micrupolyspora faeni, the organism most commonly causing farmer's lung disease (Pepys et al., 1963). In individuals showing clinical signs of the disease, precipitating antibody can sometimes, but not always, be demonstrated (Pepys and Jenkins, 1965;Grant et al., 1972;Edwards, Baker and Davies, 1974) and it is therefore clear that the presence of antibody is proof of exposure to antigen rather than evidence of disease. Culture filtrates and mycelial extracts from M. faeni have been shown to contain numerous antigens when tested against strongly precipitin-positive sera (Fletcher, Rondle and Murray, 1970;Hollingdale, 1974). The role of such antigens in the induction of extrinsic allergic alveolitis is little understood, and although farmer's lung has been widely regarded as the result of an Arthus reaction (Type I11 of Gel1 and Coombs, 1968), components of other hypersensitivity reactions such as Type I, Type I1 and particularly Type IV (Seal et al., 1968) have also been implicated.Respirable dust from hay and grain upon which M. faeni has grown may carry an array of soluble antigens derived from metabolism, and particulate antigens in the form of mycelial fragments and spores. The following work investigates the effects of these antigens and their biochemical fractions on the development of allergic pulmonary disease in the mouse in relation to the types of hypersensitivity reaction involved. MATERIALS AND METHODS Growth and harvesting of culturesA strain of M. faeni provided by Dr J. Lacey (Rothamstead Experimental Station, Harpenden, Herts.) was grown for 1, 4 or 6 weeks in liquid culture in Casein Hydrolysate Medium (Oxoid, CM 1). Litre flasks, each containing 250 ml medium were placed in an orbital incubator in darkness at 50°C. Cultures were also grown on half-strength nutrient agar for 1 week. After the addition of sodium azide to give a final concentration of 0.03 % (w/v), culture filtrate was freed of mycelium by centrifugation for 20 min. at 3000 g and room temperature). The culture filtrate (CF) was then clarified, concentrated and dialysed in an Amicon Stirred Cell Model 202 (Amicon Ltd, Lexington, Mass., USA) with DP06 and UM2 filters. The final retentate was approximately one-fortieth the volume of the original CF. Mycelium was washed twice in phosphate buffered saline ( 0 . 1 3 7~ sodium chloride in 0 . 0 6 7~ phosphate buffer, pH 7.0) by centrifuging at 3000 g for 20 min., and stored at 4°C.

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Section: Discussionmentioning

confidence: 92%

Particulate and Soluble Antigens of Micropolyspora Faeni In Experimental Allergic Alveolitis of the Mouse

Blyth¹,

Wardrop²

1977

Journal of Medical Microbiology

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“…Unlike calves [16,17] and rabbits [2, 6-8, 12, 15], guinea pigs required systemic as well as pulmonary exposure to M.faeni to become susceptible to an ex aggerated histologic response to an i.t. challenge of M. faeni.…”

Section: Discussionmentioning

confidence: 99%

“…challenge of M. faeni. This difference between guinea pigs and other animals may be due to the frequent occurrence of serum antibodies to M. faeni in calves prior to sen sitization [ 16] or to species differences.…”

Section: Discussionmentioning

confidence: 99%

Hypersensitivity Pneumonitis in Strain II Guinea Pigs I.

Schuyler¹,

Schmitt²,

Steinberg³

1982

Int Arch Allergy Immunol

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A model of hypersensitivity pneumonitis (HP) in syngeneic animals would allow experiments designed to determine immunopathogenesis of HP. Strain II guinea pigs were treated with Micropolyspora faeni and challenged with intratrachael M. faeni. Lung histology 4 days thereafter was quantitatively evaluated. Intratrachael administration of M. faeni caused interstitial and intraalveolar collections of mononuclear and polymorphonuclear cells. Sensitized (pretreated with M. faeni) animals’ lungs had more extensive pathology than unsensitized animals. 3 weeks after challenge with M. faeni, pulmonary histopathology regressed to granulomatous interstitial infiltration, similar to that found in chronic HP in humans. We conclude that the pulmonary histopathologic response of sensitized strain II guinea pigs to intratrachael M. faeni resembles human HP.

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“…Passive Cutaneous Anaphylaxis (PCA) Positive Serum Calves were sensitized with soluble M. faeni antigen as described by Wilkie et al [1973]. Sera obtained from these animals were tested for reaginic antibody by PCA reaction in calves [Niel sen et al, 1976].…”

Section: Methodsmentioning

confidence: 99%

Bovine Reaginic Antibody

Nielsen

Wilkie

1977

Int Arch Allergy Immunol

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A method of preparing an antiserum to bovine reaginic immunoglobulins is described. This procedure which relies on the ability of bovine reaginic immunoglobulin to attach to rat mast cells, results in an antiserum which upon absorption with rat and bovine materials did not react with rat serum or with bovine IgG₁, IgG₂, IgM and IgA. The antiserum did, however, react in a gel diffusion assay with PCA positive bovine globulin and a reaginic immunoglobulin-rich fraction of bovine serum. Further, anti-reaginic immunoglobulin activity was shown by binding to and degranulation of rat mast cells previously incubated with PCA positive bovine globulin. The antiserum prevented sensitization of calf skin in a PCA reaction while anti-human IgE did not.

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Hypersensitivity Pneumonitis: Experimental Production in Guinea Pigs with Antigens of <i>Micropolyspora faeni</i>

Cited by 21 publications

References 13 publications

Particulate and Soluble Antigens of Micropolyspora Faeni In Experimental Allergic Alveolitis of the Mouse

Particulate and Soluble Antigens of Micropolyspora Faeni In Experimental Allergic Alveolitis of the Mouse

Hypersensitivity Pneumonitis in Strain II Guinea Pigs I.

Bovine Reaginic Antibody

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