Hyperspectral coherent Raman imaging – principle, theory, instrumentation, and applications to life sciences

Abstract

Coherent Raman scattering microscopy such as coherent anti‐Stokes Raman scattering and stimulated Raman scattering microscopy boosts the weak Raman signal and enables us to perform label‐free visualization of the molecular distribution and its dynamical behavior in living cells and tissues with high speed. In comparison with fluorescence imaging, cells and tissues can be visualized without specifying the target molecule. In this review, we describe the characteristics of a hyperspectral coherent Raman imaging method, which is capable of acquiring both spectra and images simultaneously, and review applications to life sciences. Copyright © 2015 John Wiley & Sons, Ltd.

Recent advances in linear and non‐linear Raman spectroscopy. Part XI

“…They described the characteristics of a hyperspectral coherent Raman imaging as being capable of acquiring both spectra and images simultaneously. In comparison with fluorescence imaging, cells and tissues can be visualized without specifying the target molecule . Karuna et al used hyperspectral volumetric CARS microscopy as a quantitative volume determination with NaCl as nonresonant standard.…”

“…For the International Year of Light issue, the following reviews were published: Bersani and Lottici on Raman spectroscopy of minerals and mineral pigments in archaeometry; Buzzini and Suzuki on Forensic applications of Raman spectroscopy; Centeno on Identification of artistic materials in paintings and drawings by Raman spectroscopy; Fisk and co‐workers on Achieving optimal SERS through enhanced experimental design; Doty and co‐workers on What can Raman spectroscopy do for criminalistics? ; Gares and co‐workers on Review of explosive detection methodologies and the emergence of standoff deep UV resonance Raman; Ji, Zhao, and Ozaki on Semiconductor materials in analytical applications of surface‐enhanced Raman scattering; Kano and co‐workers on Hyperspectral coherent Raman imaging—Principle, theory, instrumentation, and applications to life sciences; Penido and co‐workers on Raman spectroscopy in forensic analysis: Identification of cocaine and other illegal drugs of abuse; Pozzi and Leona on Surface‐enhanced Raman spectroscopy in art and archaeology; Stoeckel and co‐workers on The application of Raman spectroscopy for the detection and identification of microorganisms; Yamamoto and Itoh on Why and how do the shapes of surface‐enhanced Raman scattering spectra change? Other reviews that appeared during 2016 were Cui and co‐workers on Plasmon‐driven catalysis in aqueous solutions probed by SERS spectroscopy and Chen and co‐workers on Probing single molecules and molecular aggregates.…”

Raman Under Water – Nonlinear and Nearfield Approaches for Electrochemical Surface Science

“…As a result, spectral integration times can be reduced compared to conventional Raman spectroscopy to tens of ms, rendering CARS a valuable novel tool for in situ quantitative monitoring of dynamic molecular processes on the nanoscale. Commonly, CARS is employed in the field of bio(medical) imaging and has only recently been introduced to the field of materials science. In the following, we highlight the potential CARS holds for the study of important (electro)chemical processes occurring inside nanopores, such as the catalytic chemical conversion on zeolite particles and the interaction and diffusion of water inside fuel‐cell membranes.…”

Discrimination of tumor from normal tissues in a mouse model of breast cancer using CARS spectroscopy combined with PC‐DFA methodology

“…In the last decade, coherent anti‐Stokes Raman scattering (CARS) spectroscopy has become an attractive tool for vibrational analysis of chemical and biological samples containing Raman vibrational signatures . CARS has proven to be more sensitive than spontaneous Raman spectroscopy due to the coherent scattering and thus more suited for applications that require rapid and real‐time analysis.…”