Hyperstimulation affects vascular permeability at implantation sites in the rat endometrium

Kramer, Beverley; Magan, Ansuyah; Wet, G de

doi:10.1007/bf01207741

Cited by 15 publications

(16 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These changes are in agreement with findings made by Stein and Kramer (1989), Kramer et al (1993) and Valbuena et al (1999) whose results indicate both stimulation (dilatation of the uterine horns, accumulation of uterine fluid, changes in the luminal epithelial height and microvillus border) and damage (presence of necrotic and damaged cells) of the endometrium by the gonadotropic hormones. Additional differences were observed in the cytology of the epithelial cells in the current study.…”

Section: The Effects Of Hyperstimulation On the Histology Of The Rat supporting

confidence: 93%

“…Although the markers of pregnancy are indisputable, the dating of pregnancy varies within published studies. In the current study, the presence of the mentioned markers on the morning following mating is referred to as day 0.5 of pregnancy (Stein and Kramer, 1989;Kramer et al, 1993). As the animals mate around 8 p.m. and are killed at approximately 8 a.m. the following morning, the dating of pregnancy is held to be approximately 12 hours or 0.5 day of pregnancy.…”

Section: Dating Of Pregnancymentioning

confidence: 99%

“…The daily smears were fixed in alcohol and stained using Shore's technique (Drury and Wallington, 1980) (Appendix 1). The stages of the oestrus cycle were assessed according to Kent and Smith (1945) (Kramer et al, 1993). Since it has been shown that the endometrial changes in the human female and in the rat are very similar at the time of implantation (Psychoyos and Martel, 1985), female rats in the present study have been hyperstimulated in phase with the oestrus cycle to simulate the conditions comparable to human IVF.…”

Section: Aim Of Studymentioning

confidence: 99%

“…In these two hyperstimulated animals, unattached embryos were found in the uterine lumina, but implanting embryos were never located. The possible reason for this exception could be the alteration in the hormonal levels known to occur in hyperstimulated animals (Kramer et al, 1990), their detrimental effect on the morphology of the endometrium as well as on the passage of the ova down the uterine tubes (Kramer et al, 1993) and an individual response of these animals to the abovementioned changes, that had altered the endocrine environment and uterine morphology due to the supraphysiological hormonal environment.…”

Section: Differences In Tgfmentioning

confidence: 99%

“…Administration of exogenous hormones is found to increase the amount of circulating oestradiol prior to implantation (Kramer, 1990), affect the progesterone: oestradiol ratio (Kramer et al, 1993, Kramer andde Wet, 1994), decrease the vascular permeability of the endometrium (Kramer, 1997), prevent decidualization of the subepithelial stromal cells (Stein and Kramer, 1989) and reduce carbohydrates in the glycocalyx of the endometrium (Kramer and de Wet, 1994;Peverini and Kramer, 1995).…”

mentioning

confidence: 99%

See 4 more Smart Citations

The effect of hyperstimulation on transforming growth factor β1 and β2 in the rat uterus: possible consequences for embryo implantation

Jovanović

Kramer

2010

Fertility and Sterility

Self Cite

View full text Add to dashboard Cite

Section: The Effects Of Hyperstimulation On the Histology Of The Rat supporting

confidence: 93%

Section: Dating Of Pregnancymentioning

confidence: 99%

Section: Aim Of Studymentioning

confidence: 99%

Section: Differences In Tgfmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

The effect of hyperstimulation on transforming growth factor β1 and β2 in the rat uterus: possible consequences for embryo implantation

Jovanović

Kramer

2010

Fertility and Sterility

Self Cite

View full text Add to dashboard Cite

Changes in vascular permeability and deciduoma formation during the peri-implantation period of the rat in response to exogenous gonadotropins

Kramer

1997

Anat. Rec.

Self Cite

View full text Add to dashboard Cite

Background Permeability of thin‐walled vessels of the endometrium could be a prerequisite for deciduoma formation. Exogenous gonadotropins alter endometrial morphology and inhibit vascular permeability on the day of implantation. The aim of this study was to investigate the effect of exogenous gonadotropins on vascular permeability in the peri‐implantation period and relate this to deciduoma formation. Methods Female rats were hyperstimulated with follicle‐stimulating hormone and human chorionic gonadotropin prior to mating. Control animals were not injected. Endometrial tissue was collected at 4.5, 5.5, and 6.5 days after mating. Tissue was processed for electron microscopy. Results The presence of thin‐walled fenestrated vessels and decidualised stromal cells were found in all control groups. With the exception of one fenestration, which occurred in a vessel from one hyperstimulated animal on 5.5 days of pregnancy, no fenestrations were found in the vessels of hyperstimulated animals. Decidualisation of stromal cells did not occur in these animals. Conclusion Hyperstimulation with exogenous gonadotropins inhibits vascular permeability and subsequent decidualisation. Implantation thus fails to occur. Anat. Rec. 247:20–24 © Wiley‐Liss, Inc.

show abstract

Effect of exogenous gonadotropins on gonadotrophs of the rat pituitary gland

Thomadakis

Kramer

1999

Anat. Rec.

Self Cite

View full text Add to dashboard Cite

In the human in vitro fertilization (IVF) program a variety of superovulation regimens have been employed to promote follicular stimulation and the recruitment of supernumerary oocytes. This therapy, however, disturbs serum concentrations of estradiol and progesterone and may disrupt the normal feedback systems of the hypothalamo-pituitary axis. This study examines the effects of hyperstimulation on the pituitary gonadotrophs and circulating gonadotrophins. FSH and human chorionic gonadotropin (hCG) were administered to normal cycling female Sprague-Dawley rats (n = 12) in phase with their estrous cycle. Control rats (n = 12) were injected with saline. In both the experimental and control groups, six rats were mated on the evening of proestrus and killed 12 hr later, while six animals were killed prior to mating. Blood was collected at the time of sacrifice for radioimmunoassay. The pituitary glands were removed, processed for light microscopy and serially sectioned. Immunocytochemistry for LH and FSH was carried out to determine the area occupied by these cell types. Data were statistically analyzed. Findings were correlated with circulating levels of LH, FSH, estradiol and progesterone. RIA revealed that the concentration of LH, FSH, estradiol and progesterone were significantly different with respect to hyperstimulation and mating. In addition the area occupied by LH and FSH cells was significantly different with respect to both hyperstimulation and mating. Hyperstimulation affects gonadotroph content, as well as gonadotropin and sex steroid hormone concentrations and together with other factors, may disrupt the ideal environment required for implantation.

show abstract

Hyperstimulation affects vascular permeability at implantation sites in the rat endometrium

Cited by 15 publications

References 18 publications

The effect of hyperstimulation on transforming growth factor β1 and β2 in the rat uterus: possible consequences for embryo implantation

The effect of hyperstimulation on transforming growth factor β1 and β2 in the rat uterus: possible consequences for embryo implantation

Changes in vascular permeability and deciduoma formation during the peri-implantation period of the rat in response to exogenous gonadotropins

Effect of exogenous gonadotropins on gonadotrophs of the rat pituitary gland

Contact Info

Product

Resources

About