Hypertension and Aging Affect Liver Sulfur Metabolism in Rats

Szlęzak, Dominika; Bronowicka-Adamska, Patrycja; Hutsch, Tomasz; Ufnal, Marcin; Wróbel, Maria

doi:10.3390/cells10051238

Cited by 17 publications

(10 citation statements)

References 51 publications

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“…But a study shows the effect of aging on rat liver and the regulation of sulfur compounds. This study concludes that there is a mixed trend observed in the production of sulfur compounds with aging 36 .…”

Section: Methodsmentioning

confidence: 57%

A pilot study for the prediction of liver function related scores using breath biomarkers and machine learning

Patnaik

Lin

Agarwal

et al. 2022

Sci Rep

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Volatile organic compounds (VOCs) present in exhaled breath can help in analysing biochemical processes in the human body. Liver diseases can be traced using VOCs as biomarkers for physiological and pathophysiological conditions. In this work, we propose non-invasive and quick breath monitoring approach for early detection and progress monitoring of liver diseases using Isoprene, Limonene, and Dimethyl sulphide (DMS) as potential biomarkers. A pilot study is performed to design a dataset that includes the biomarkers concentration analysed from the breath sample before and after study subjects performed an exercise. A machine learning approach is applied for the prediction of scores for liver function diagnosis. Four regression methods are performed to predict the clinical scores using breath biomarkers data as features set by the machine learning techniques. A significant difference was observed for isoprene concentration (p < 0.01) and for DMS concentration (p < 0.0001) between liver patients and healthy subject’s breath sample. The R-square value between actual clinical score and predicted clinical score is found to be 0.78, 0.82, and 0.85 for CTP score, APRI score, and MELD score, respectively. Our results have shown a promising result with significant different breath profiles between liver patients and healthy volunteers. The use of machine learning for the prediction of scores is found very promising for use of breath biomarkers for liver function diagnosis.

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Section: Methodsmentioning

confidence: 57%

A pilot study for the prediction of liver function related scores using breath biomarkers and machine learning

Patnaik

Lin

Agarwal

et al. 2022

Sci Rep

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“…EMVs were more effective than EEXs on regulating GSSG and klotho. MDA and GSSG have been implicated in aging and served as oxidative stress markers, and high levels of MDA and GSSG were found in the hippocampus of aged rat [36, 37]. Klotho gene is widely accepted as an anti-aging gene with anti-oxidative stress and anti-inflammation functions, and klotho overexpression could extend life span of mice [38].…”

Section: Discussionmentioning

confidence: 99%

“…Aging associated biomarkers including MDA, GSSG and klotho levels were detected according to the manufacturer’s instructions (MDA: Beyotime, cat# S0131M, GSSG: Beyotime, cat# S0053, Klotho: ZCI Bio, cat# ZC-31968) as previously reported, and TERT level was measured by q-PCR [36, 37, 39, 40]. The detailed procedures could be found in the supplementary materials.…”

Section: Methodsmentioning

confidence: 99%

Endothelial cell derived microvesicles modulate cerebrovascular and brain aging via miR-17-5p and serve as potential biomarkers for vascular aging

Zhang

Xie

Zhao

et al. 2023

Preprint

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Vascular aging, characterized by brain endothelial cells (ECs) senescence and dysfunction, has been known to lead to various age-related cerebrovascular and neurodegenerative diseases. However, its underlying mechanisms remain elusive. ECs derived microvesicles (EMVs) and exosomes (EEXs) carry the characteristics of parent cells and transfer their contents to modulate the functions of recipient cells, holding the potential to evaluate or regulate vascular aging. Here, we found that young or aged ECs released EMVs were more effective than their released EEXs on alleviating or aggravating mice cerebrovascular and brain aging as indicated by SA-β-gal staining, cerebral blood flow, blood brain barrier function, aging related markers and cognitive ability test. We further identified that these EMVs regulated cerebrovascular and brain aging by transferring miR-17-5p and could modulate ECs senescence and functions via miR-17-5p/PI3K/Akt pathway. Plasma EMVs and their contained miR-17-5p (EMV-miR-17-5p) were significantly increased or decreased in the elderly, and were closely correlated with vascular aging. Receiver Operating Characteristic (ROC) analysis showed that the area under the curve was 0.724 for EMVs, 0.77 for EMV-miR-17-5p and 0.815 for their combination for distinguishing vascular aging. Our results revealed the novel roles for EMVs that could more effectively modulate vascular and brain aging than EEXs by regulating ECs functions through miR-17-5p/PI3K/Akt pathway, and also suggested that EMVs and EMV-miR-17-5p represent promising biomarkers and therapeutic targets for vascular aging.

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“…Alterations in cellular metabolism play an important role in controlling and steering the inflammatory state of both endothelial cells and immune cells, and this change has been shown to be related to hypertension in multiple studies. [85][86][87] Many studies have demonstrated the brain-gut connection pathway of hypertension, but relatively little is known about the role of gut-kidney connection, in particular, whether changes in renal function are associated with increased intestinal permeability and changes in the expression of inflammatory genes. Our results present candidate pathways and genes to explore the relationship between metabolism, inflammation, and hypertension on the gut-kidney axis.…”

Section: Discussionmentioning

confidence: 99%

Calcitriol ameliorates damage in high-salt diet-induced hypertension: Evidence of communication with the gut–kidney axis

Ding

Xiao

Jiang

et al. 2021

Exp Biol Med (Maywood)

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Several studies have established a link between high-salt diet, inflammation, and hypertension. Vitamin D supplementation has shown anti-inflammatory effects in many diseases; gut microbiota is also associated with a wide variety of cardiovascular diseases, but potential role of vitamin D and gut microbiota in high-salt diet-induced hypertension remains unclear. Therefore, we used rats with hypertension induced by a high-salt diet as the research object and analyzed the transcriptome of their tissues (kidney and colon) and gut microbiome to conduct an overall analysis of the gut–kidney axis. We aimed to confirm the effects of high salt and calcitriol on the gut–kidney immune system and the composition of the intestinal flora. We demonstrate that consumption of a high-salt diet results in hypertension and inflammation in the colon and kidney and alteration of gut microbiota composition and function. High-salt diet-induced hypertension was found to be associated with seven microbial taxa and mainly associated with reduced production of the protective short-chain fatty acid butyrate. Calcitriol can reduce colon and kidney inflammation, and there are gene expression changes consistent with restored intestinal barrier function. The protective effect of calcitriol may be mediated indirectly by immunological properties. Additionally, the molecular pathways of the gut microbiota-mediated blood pressure regulation may be related to circadian rhythm signals, which needs to be further investigated. An innovative association analysis of the microbiota may be a key strategy to understanding the association between gene patterns and host.

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Hypertension and Aging Affect Liver Sulfur Metabolism in Rats

Cited by 17 publications

References 51 publications

A pilot study for the prediction of liver function related scores using breath biomarkers and machine learning

A pilot study for the prediction of liver function related scores using breath biomarkers and machine learning

Endothelial cell derived microvesicles modulate cerebrovascular and brain aging via miR-17-5p and serve as potential biomarkers for vascular aging

Calcitriol ameliorates damage in high-salt diet-induced hypertension: Evidence of communication with the gut–kidney axis

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