Hyperthermia as a Method to Increase the Radiosensitivity of Tumor Cells Unsusceptible to Pharmacological Radiosensitizers

Хохлова, А. В.; Yakimova, A. O.; Mosina, V.; Selivanova, E. I.; Kabakov, Alexander E.

doi:10.1134/s1062359021110042

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2022

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“…In particular, the use of enzymes for cell isolation can affect the expression of cell-surface molecules [ 144 ]. Hyperthermia is used to increase enzymatic processing efficiency and may be applied as a method of cellular isolation, however, it leads to cellular stress [ 145 , 146 ].…”

Section: Limitationsmentioning

confidence: 99%

Intraoperative Creation of Tissue-Engineered Grafts with Minimally Manipulated Cells: New Concept of Bone Tissue Engineering In Situ

et al. 2022

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Transfer of regenerative approaches into clinical practice is limited by strict legal regulation of in vitro expanded cells and risks associated with substantial manipulations. Isolation of cells for the enrichment of bone grafts directly in the Operating Room appears to be a promising solution for the translation of biomedical technologies into clinical practice. These intraoperative approaches could be generally characterized as a joint concept of tissue engineering in situ. Our review covers techniques of intraoperative cell isolation and seeding for the creation of tissue-engineered grafts in situ, that is, directly in the Operating Room. Up-to-date, the clinical use of tissue-engineered grafts created in vitro remains a highly inaccessible option. Fortunately, intraoperative tissue engineering in situ is already available for patients who need advanced treatment modalities.

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Section: Limitationsmentioning

confidence: 99%