Hyperthermia inhibits cellular function and induces immunogenic cell death in renal cell carcinoma

Abstract

Background In recent years, hyperthermia has been widely applied as a novel strategy for cancer treatment due to its multiple antitumour effects. In particular, the potential influences of hyperthermia on the tumour immune microenvironment may improve the efficacy of immunotherapies. However, the effect of hyperthermia on renal cell carcinoma (RCC) has not been well characterized until now. Methods In the present study, we primarily evaluated the effects of hyperthermia on cellular function via cellular proliferation, migration, invasion and apoptosis assays. In addition, the influence of hyperthermia on the immunogenicity of RCC cells was analysed using flow cytometry analysis, enzyme-linked immunosorbent assays, and immunofluorescent (IF) staining. Results Our results demonstrate that hyperthermia significantly inhibits RCC cell proliferation, migration, and invasion and promotes cell apoptosis. In addition, we verified that hyperthermia improves the immunogenicity of RCC cells by inducing immunogenic cell death. Conclusion Our findings suggest that hyperthermia is a promising therapeutic strategy for RCC.

Ultrasound -Induced Thermal Effect Enhances the Efficacy of Chemotherapy and Immunotherapy in Tumor Treatment

“…Mild-hyperthermia therapy is a therapeutic approach that utilizes mild heat to enhance the permeability of tumor blood vessels and expand the pores of tumor microvessels, thereby facilitating improved penetration of therapeutic drugs and minimizing damage to surrounding normal tissues. 27–30 Clinical evidence has consistently demonstrated that combining hyperthermia with chemotherapy effectively enhances local tumor control rates and prolongs patient survival. 31 Xia et al synthesized photo-responsive gold nanoparticles (AuNPs), which exhibited laser-induced accumulation towards the tumor and generated photothermal effects at the tumor site.…”

Water‐filtered infrared A radiation hyperthermia combined with immunotherapy for advanced gastrointestinal tumours

“… 9 Due to the deviation from the optimal temperature, the efficiency of many organelles and sub‐organelles is weakened. 10 Inhibition of certain transmembrane proteins disrupts ion transport, causing intracellular accumulation of Ca 2+ , which leads to endoplasmic reticulum stress and caspase‐dependent apoptosis. 11 Secondly, from an immunological perspective, hyperthermia significantly enhances anti‐tumour immune responses, encompassing both specific and non‐specific immunity.…”