Hyperthermia‐treated mesenchymal stem cells exert antitumor effects on human carcinoma cell line

Cho, Jung Ah; Park, Ho; Kim, Hee Kyung; Lim, Eic Ju; Seo, Sang Won; Choi, Joong Sub; Lee, Kyo Won

doi:10.1002/cncr.24032

Cited by 42 publications

(57 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It has been demonstrated that hyperthermia treatment of human MSCs produced antitumor effect. Cultivation of cancer cells in the conditioned medium collected from heated MSCs exerted a suppressive effect on tumor cell growth, suggesting that hyperthermia enables tumor stromal cells to provide a sensitizing environment for tumor cells (Cho et al 2009). Further experiments are required to confirm these findings.…”

Section: Discussionmentioning

confidence: 97%

Sublethal heat shock induces premature senescence rather than apoptosis in human mesenchymal stem cells

Алексеенко

Zemelko

Домнина

et al. 2014

Cell Stress and Chaperones

View full text Add to dashboard Cite

Stem cells in adult organism are responsible for cell turnover and tissue regeneration. The study of stem cell stress response contributes to our knowledge on the mechanisms of damaged tissue repair. Previously, we demonstrated that sublethal heat shock (HS) induced apoptosis in human embryonic stem cells. This study aimed to investigate HS response of human adult stem cells. Human mesenchymal stem cells (MSCs) cultivated in vitro were challenged with sublethal HS. It was found that sublethal HS did not affect the cell viability assessed by annexin V/propidium staining. However, MSCs subjected to severe HS exhibited features of stress-induced premature senescence (SIPS): irreversible cell cycle arrest, altered morphology, increased expression of senescence-associated β-galactosidase (SA-β-gal) activity, and induction of cyclin-dependent kinase inhibitor p21 protein. High level of Hsp70 accumulation induced by sublethal HS did not return to the basal level, at least, after 72 h of the cell recovery when most cells exhibited SIPS hallmarks. MSCs survived sublethal HS, and resumed proliferation sustained the properties of parental MSCs: diploid karyotype, replicative senescence, expression of the cell surface markers, and capacity for multilineage differentiation. Our results showed for the first time that in human MSCs, sublethal HS induced premature senescence rather than apoptosis or necrosis. MSC progeny that survived sublethal HS manifested stem cell properties of the parental cells: limited replicative life span and multilineage capacity.

show abstract

Section: Discussionmentioning

confidence: 97%

Sublethal heat shock induces premature senescence rather than apoptosis in human mesenchymal stem cells

Алексеенко

Zemelko

Домнина

et al. 2014

Cell Stress and Chaperones

View full text Add to dashboard Cite

show abstract

“…More importantly, a study was carried out showing that MSCs within the tumor stroma promote breast cancer metastasis by CCL5 signaling through the chemokine receptor CCR5 expressed in the tumors (18). Our previous studies demonstrated that cytokines secreted by MSCs can influence tumor cell growth (43,44). Therefore, manipulation of the interaction between MSCs within the tumor stroma and tumor cells might improve conventional cancer therapy.…”

Section: Discussionmentioning

confidence: 99%

Exosomes from breast cancer cells can convert adipose tissue-derived mesenchymal stem cells into myofibroblast-like cells

Cho

Park²,

Lim³

et al. 2011

Int J Oncol

198

126

View full text Add to dashboard Cite

Abstract. Exosomes are small membrane vesicles secreted into the extracellular environment by various types of cells, including tumor cells. Exosomes are enriched with a discrete set of cellular proteins, and therefore expected to exert diverse biological functions according to cell origin. Mesenchymal stem cells (MSCs) possess the potential for differentiation into multilineages and can also function as precursors for tumor stroma including myofibroblast that provides a favorable environment for tumor progression. Although a close relationship between tumor cells and MSCs in a neoplastic tumor microenvironment has already been revealed, how this communication works is poorly understood. In this study, we investigated the influence of tumor cell-derived exosomes on MSCs by treating adipose tissuederived MSCs (ADSCs) with breast cancer-derived exosomes. The exosome-treated ADSCs exhibited the phenotypes of tumor-associated myofibroblasts with increased expression of α-SMA. Exosome treatment also induced increased expression of tumor-promoting factors SDF-1, VEGF, CCL5 and TGFβ. This phenomenon was correlated with increased expression of TGFβ receptor I and II. Analysis of SMAD2, a key player in the TGFβ receptor-mediated SMAD pathway, revealed that its phosphorylation was increased by exosome treatment and was inhibited by treatment with SB431542, an inhibitor of the SMAD-mediated pathway, resulting in decreased expression of α-SMA. Taken together, our results show that tumor-derived exosomes induced the myofibroblastic phenotype and functionality in ADSCs via the SMAD-mediated signaling pathway. In conclusion, this study suggests that tumor-derived exosomes can contribute to progression and malignancy of tumor cells by converting MSCs within tumor stroma into tumor-associated myofibroblasts in the tumor microenvironment.

show abstract

“…Furthermore, human fetal skin-derived MSCs inhibited the growth of MCF-7 breast cancer cells in vitro. 52 Cho et al 53 reported that hyperthermia-treated MSCs (heattreated MSCs) exerted antitumor effects on human carcinoma cells by producing various cytokines such as interleukin (IL) ( Table 1).…”

Section: Non-engineered Stem Cells Transplantation Strategiesmentioning

confidence: 99%

“…It is said that the tumor suppression effect was due to various cytokines produced by AM-hMSCs such as granulocyte macrophage colony-stimulating factor, IL-6, neurotrophin 3, CCL18 (a chemokine), macrophage colony-stimulating factor, brain-derived neutrophic factor, granulocyte chemotactic protein (GCP-2) and conserved dopomine neutrophic factors. 53 In detail, AM-hMSCs may mediate the tumortargeting effects of immunocytes by regulating the expressions of various cytokines such as tumor necrosis factor-a and ILs. 55 For instance, ILs may help immune systems by activating natural killer cells that can directly target human cancers.…”

Section: Non-engineered Stem Cells Transplantation Strategiesmentioning

confidence: 99%

Potential antitumor therapeutic strategies of human amniotic membrane and amniotic fluid-derived stem cells

et al. 2012

Cancer Gene Ther

View full text Add to dashboard Cite

Hyperthermia‐treated mesenchymal stem cells exert antitumor effects on human carcinoma cell line

Abstract: BACKGROUND:Mesenchymal stem cells (MSCs) possess the potential for differentiation into multilineages.

Cited by 42 publications

References 47 publications

Sublethal heat shock induces premature senescence rather than apoptosis in human mesenchymal stem cells

Sublethal heat shock induces premature senescence rather than apoptosis in human mesenchymal stem cells

Exosomes from breast cancer cells can convert adipose tissue-derived mesenchymal stem cells into myofibroblast-like cells

Potential antitumor therapeutic strategies of human amniotic membrane and amniotic fluid-derived stem cells

Contact Info

Product

Resources

About