Hyperthermic enhancement of chromosome damage and lack of effect on sister-chromatid exchanges induced by bleomycin in Chinese hamster cells in vitro

Vig, Baldev K.

doi:10.1016/0027-5107(79)90136-2

Cited by 11 publications

References 10 publications

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A comparison of induced sister chromatid exchange levels in chinese hamster ovary cells and cultured human lymphocytes

Morgan

Crossen

1982

Environ. mutagen

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Human lymphocytes and Chinese hamster ovary cells were exposed to DNA damaging agents for two cell cycles, and the induced sister chromatid exchange (SCE) rate was compared. CHO cells showed a significant increase in SCE following treatment with bleomycin and vincristine whereas human lymphocytes did not. Both CHO cells and lymphocytes showed an increase in SCE with 5-bromodeoxyuridine (BrdUrd) and tritiated uridine (3H-Urd) but the increase was greater in CHO cells. SCE levels were similar after exposure to proflavine and colcemid did not increase the exchange frequency in either cell type. Apparently CHO cells are more sensitive than human lymphocytes to the actions of bleomycin, vincristine, BrdUrd, and 3H-Urd. SCE analysis in response to mutagens and carcinogens should therefore be based on more than one cell type.

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A comparison of induced sister chromatid exchange levels in chinese hamster ovary cells and cultured human lymphocytes

Morgan

Crossen

1982

Environ. mutagen

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Neuroblast of the grasshopper embryo as a new mutagen test system. Ii. Chromosome breakage induced by in vitro exposure of embryos to the direct‐acting mutagens 4NQO, MNNG, adriamycin, and bleomycin

Liang

Gaulden

1982

Environ. mutagen

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The dose-response for the induction of acentric chromosome fragments was determined in neuroblasts of the grasshopper embryo (Chortophaga viridifasciata De Geer, Orthoptera: Acrididae) exposed in vitro to four direct-acting chemical known to be mutagenic, clastogenic, and carcinogenic: 4-nitroquinoline-1-oxide (4NQO), N-methyl-N-nitro-N-nitrosoguanidine (MNNG), Adriamycin (ADM), and bleomycin (BLM). After a 1-hr exposure followed by a 3-hr recovery period (untreated cell cycle time is 4 hr), acentric fragments were observed at doses down to 1 microM 4NQO, 1.25 microM MNNG, and 0.125 microM ADM and BLM. After an 8-hr continuous exposure, acentric fragments were induced by 4NQO at a dose as low as 0.125 microM. These low concentrations also reduced the number of dividing cells. No chromosome aberrations or mitotic effects were observed in untreated embryos or in those exposed only to the solvent dimethyl sulfoxide. Because of the short cell cycle and the sensitivity of the neuroblast to the induction of acentric chromosome fragments by chemical clastogens, a minimum of time is needed to perform the test. From a comparison with the prominent clastogen test systems currently used, it is concluded that the grasshopper neuroblast test is the fastest and that it detects some agents that some systems do not. Grasshoppers have a worldwide distribution. If neuroblasts of other species prove to be as sensitive to mutagens as those of Chortophaga, investigators in many countries would have available a eukaryotic mutagen test system that is simple, fast, reproducible, and inexpensive.

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Replication Bypass SCE Mechanisms and the Induction of SCE by Single-Strand Adducts or Lesions of DNA

Da¹

1984

Sister Chromatid Exchanges

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Hyperthermic enhancement of chromosome damage and lack of effect on sister-chromatid exchanges induced by bleomycin in Chinese hamster cells in vitro

Cited by 11 publications

References 10 publications

A comparison of induced sister chromatid exchange levels in chinese hamster ovary cells and cultured human lymphocytes

A comparison of induced sister chromatid exchange levels in chinese hamster ovary cells and cultured human lymphocytes

Neuroblast of the grasshopper embryo as a new mutagen test system. Ii. Chromosome breakage induced by in vitro exposure of embryos to the direct‐acting mutagens 4NQO, MNNG, adriamycin, and bleomycin

Replication Bypass SCE Mechanisms and the Induction of SCE by Single-Strand Adducts or Lesions of DNA

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